RBL-2H3
Studies on the Degranulation of RBL 2H3 Cells Induced by Traditional Chinese Medicine Injections
... induce RBL-2H3 degranulation, and below these concentration, the enzyme release rate drop ...cause RBL-2H3 cell degranulation, we ad- justed the pH of Tyrode’s buffer from ...induce ...
9
Controlled-release formulation of antihistamine based on cetirizine zinc-layered hydroxide nanocomposites and its effect on histamine release from basophilic leukemia (RBL-2H3) cells
... In this study, a new, simple, and direct method for the inter- calation of anions into ZLH was realized, using zinc oxide as the starting material. The resulting nanocomposite shows long-range, nano-layered structure ...
13
The role of the phosphatidylinositol transfer protein and the small GTP-ASE ARF in regulated exocytosis from RBL-2H3 mast cells
... Phospholipase D is activated in many cell-types including mast cells and RBL- 2H3 cells activated by anti-lgE (115,116). Early work demonstrated that activation was maximal only in the presence of cytosol, ...
195
The role of k+ channels in the signal transduction mechanisms of antigen-induced degranulation in the rat basophilic leukaemia (rbl-2h3) cell line
... Although antigen-induced Ca^"^ oscillations are diverse in frequency, amplitude and shape, the Ca^^ response is clearly dependent on the antigen concentration (Fig. 6.2). The magnitude of the Ca^^ responses generally ...
239
Signal transduction by the high affinity receptor for immunoglobulin E, FcepsilonRI, in the mast cell line RBL-2H3
... Ras is also involved in FceR l regulation of the Nuclear Factor of Activated T cells (NFAT) transcription factor. NFAT regulates the promoters of multiple cytokines, grow th fac[r] ...
264
Synthetic Alkylamide 15 and the Required Structural Components for Mast Cell Inhibition.
... treated RBL-2H3 cells with 1 µM A23187 or 5 ng/mL DNP-BSA in the absence or presence of indicated concentrations of A15 and measured the percent release of β- hex ...
81
Aberrant maturation of mutant perforin underlies the clinical diversity of hemophagocytic lymphohistiocytosis
... in RBL cells has a different mobility on reducing and nonreducing SDS-PAGE than endog- enous perforin from NK92 cells or human perforin expressed in RBL ...in RBL-1 cells differ from those reported ...
12
Regulation of actin and secretion by Rho and calmodulin
... (W yczolkowska et a l 1986). But again these suffer from the drawbacks already listed. Passive sensitisation o f the immortalised mast cell line RBL-2H3 with IgE directed against dinitrophenol (anti-DNP ...
197
Derivation of ligands for the complement C3a receptor from the C-terminus of C5a
... 3.4 Rat Basophilic Leukemia Cell Degranulation assay RBL-2H3 cells transfected with either human C5a1 or human C3a receptor Cain and Monk, 2002 were used to confirm hits detected in HMDM[r] ...
21
RBL cells as models for in vitro studies of mast cells and basophils
... 1981, RBL-2H3 cells have been widely used as a mast cell ...the RBL cells as an attractive mast cell model for drug ...three RBL reporter cell lines (RS-ATL8, NFAT-DsRed, and NPY-mRFP) have ...
39
Palmitoylethanolamide counteracts substance P-induced mast cell activation in vitro by stimulating diacylglycerol lipase activity
... SP-induced RBL-2H3 cell de- granulation, we showed that an inhibitor of DAGLs pre- vented both PEA stimulation of 2-AG levels and PEA inhibition of ...in RBL-2H3 cell mem- branes, and human ...
16
PubMedCentral-PMC5096925.pdf
... receptors bound to IgE specific for a different Ag. These heterolo- gous complexes should then be unimpeded to trigger MC degran- ulation. To validate this notion, we sensitized RBL-2H3 cells with a mixture ...
16
Upregulation of Mas-related G Protein coupled receptor X2 in asthmatic lung mast cells and its activation by the novel neuropeptide hemokinin-1
... This suggests that if HK-1 induces degranulation in human MCs, it does so via the activation of a different GPCR. The neuropeptide substance P (SP) activates human culture-derived MCs, LAD2 cells and skin MCs via MRGPRX2 ...
5
Induction of Interleukin-4 and Interleukin-5 Expression in Mast Cells Is Inhibited by Glucocorticoids
... the RBL-2H3 mast cell line in the present study demonstrates unequivocally that these inhib- itory effects of glucocorticoids on IL-5 expression are directly on the mast ...
6
Allergens in red ginseng extract induce the release of mediators associated with anaphylactoid reactions
... RBL-2H3 cell extraction was used to screen the com- ponents binding to RBL-2H3 cells. Twenty major gin- senosides were identified from RGE (Fig. 2a), nine of which [Rg1, Rb1, Re, Rf, Rg2, Rd, ...
14
Potential in vitro anti allergic, anti inflammatory and cytotoxic activities of ethanolic extract of Baliospermum montanum root, its major components and a validated HPLC method
... from RBL-2H3 cells and cyto- toxic activity against cancerous liver cells of ...from RBL-2H3 cells, anti-inflammatory activity by detect- ing the inhibition of nitric oxide (NO) production ...
12
Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L
... in RBL-2H3 ...DNP-IgE-sensitized RBL-2H3 cells were pre-incubated with various concentration of ALBE for 30 min and then incubated with antigen (DNP-BSA) for 15 min in order to measure the ...
11
4682.pdf
... The yeast and mammalian isoforms of phosphatidylinositol transfer protein can all restore phospholipase C-mediated inositol lipid signaling in cytosol-depleted RBL- 2H3 and HL-60 cells.[r] ...
276
The role of glycosylphosphatidylinositol phospholipase D (GPI-PLD) in type one hypersensitivity
... The GPI-PLD activity assay is based on the ability of GPI-PLD enzyme to hydrolyse the GPI- anchor of the membrane-form variant surface glycoprotein (mfVSG), which is labeled with tritiated myristic acid. The products ...
322
Mast Cell Corticotropin-Releasing Factor Receptor Subtypes, CRF1 and CRF2, Differentially Modulate Stress-induced Intestinal Permeability.
... WT BMMCs and RBL-2H3 cells were stained for CRF2 expression (green) and with a nuclear stain, TO-PRO3 (blue).. Histological and functional analysis of bone marrow-derived mast cells. Wi[r] ...
107