Reaction components for semi-quantitative RT-PCR
Real-time quantitative RT -PCR (Taqman)
... for RT-PCR, it is recommended to prepare a master reagent ...separate PCR reaction in order to detect any genomic DNA contamination that may be present within the RNA ...
6
CiteSeerX — Review Quantitative RT-PCR: Pitfalls and Potential
... tative RT-PCR ...the PCR: P = T(1 + E) n (Equation ...the reaction efficiency (E) and initial amount of target (T) to be deter- ...these reaction curves were obtained by removing ...
12
Semi Quantitative PCR for Quantification of Hepatotoxic Cyanobacteria
... The amplifications, reaction controls and visualization of amplified DNA in agarose gel were carried out as pre- viously described. The linear regression equation for each assay was ob- tained using SAS ...
5
Single-Cell Gene-Expression Analysis by Quantitative RT-PCR
... into RT-PCR ...to reaction buffer, eliminating the need for an RNA isolation step; ribonuclease inhibitors were used to protect transcripts from degradation by RNases released on cell ...thermostable ...
225
quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476
... Real-time semi-quantitative TaqMan ® PCR procedure for the determination of the presence of sequence-containing DNA in Rice species DNA extracted from Rice grain ...
10
Measurement of the Infection and Dissemination of Bluetongue Virus in Culicoides Biting Midges Using a Semi-Quantitative RT-PCR Assay and Isolation of Infectious Virus
... 20% for days 7–11 post-infection) and C. nubeculosus (0–4%) again agreed closely with previous studies of these colony lines [21,22,35]. The findings of this study raise significant issues regarding the inference of ...
8
Quantitative real-time RT-PCR of CD24 mRNA in the detection of prostate cancer
... One of these markers is CD24, which was recently described by Kristiansen et al. based on RNA expression profiling. 102 tissue samples from the prostate and 31 samples from lymph node metastases were examined. The ...
7
Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays
... situ RT-PCR ...of PCR cycles used was set at 20, within the linear range of amplification defined by real-time quantitative RT-PCR (data not ...
5
A semi-quantitative RT-PCR method to measure thein vivo effect of dietary conjugated linoleic acid on porcine muscle PPAR gene expression
... a semi-quantitative RT-PCR method was developed using cDNA normalized against the housekeeping genes cyclophilin and β- ...expensive PCR instruments with real-time fluorescent ...
9
Optimisation of qualitative and semi-quantitative detection of genetically modified crops by PCR
... for semi-quantitative detection of the amount of DNA of ...For semi-quantitative PCR we have chosen sampling of the amplifi cation product in the 30 th cycle of the PCR ...using ...
6
Application of real-time quantitative RT-PCR for improving the diagnosis, treatment, and control of bovine Anaplasmosis
... A semi-parametric survival analysist was performed to analyze the variation when each respective diagnostic assay first detected ...to RT-PCR negative outcomes for each of the antibiotic treatment ...
167
Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection
... 4. Jorgensen SM, Kleveland EJ, Grimholt U, Gjoen T: Validation of reference genes for real-time polymerase chain reaction studies in Atlantic salmon. Mar Biotechnol 2006, 4:398-408. 5. Julin K, Johansen LH, Sommer ...
5
The level of embryonation influences detection of Ostertagia ostertagi eggs by semi quantitative PCR
... chloride; PCR, polymerase chain reaction; qPCR, real-time semi-quantitative polymerase chain reaction; rDNA, ribosomal DNA; SEM, standard error of the mean; SNP, single nucleotide ...
8
Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real time quantitative RT PCR (RT qPCR)
... Real-time quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR) is the most sensitive, and valuable technique for rare mRNA ...
12
QIAGEN OneStep RT-PCR Handbook
... OneStep RT-PCR Kit This protocol serves as a guideline for one-step ...and PCR are carried out sequentially in the same tube. All components required for both reactions are added during setup, ...
44
Expressed Alu repeats as a novel, reliable tool for normalization of real time quantitative RT PCR data
... in RT-qPCR based on the principle that the differential expression of a small num- ber of genes bearing the EAR will not influence EAR abundance in the ...(RT) reaction with total RNA at a final ...
12
Rapid semi automated quantitative multiplex tandem PCR (MT PCR) assays for the differential diagnosis of influenza like illness
... individual reaction tubes containing nested specific PCR primers as template for the second step reaction [11] which was performed using a liquid handling robotics system provided by AusDiag- nostics ...
12
The efficacy of quantitative fluorescent-polymerase chain reaction (QF-PCR) in the diagnosis of prenatal aneuploidy
... karyotyping, PCR, and FISH—was completely reliable for detecting an abnormal karyotype, but that the best protocol for an interpretable result was to use PCR and karyotyping, or FISH and karyotyping, and ...
6
Quantitative PCR Systems
... Chain Reaction (“PCR”) process, to practice the PCR process for internal research and development using this ...limited PCR license in accordance with the label rights accompanying such ...the ...
12
Introduction to Quantitative PCR
... of reaction efficiency changes between the same reaction performed on different plates, because you are only comparing the delta- Cts between runs rather than directly comparing Ct ...
114