Reactivity with Hydrogen Ions using ESI-MS
Identification and characterization of in vivo, in vitro and reactive metabolites of vandetanib using LC–ESI–MS/MS
... Phase II vandetanib in vivo metabolites: glucuronic acid conjugates In vivo phase II metabolic reaction was direct conjugation of vandetanib with glucuronic acid. VC651 was located in the aqueous layer in a very small ...
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Absolute quantification of endogenous angiotensin II levels in human plasma using ESI-LC-MS/MS
... LC MS/MS analysis All LC-MS/MS analysis were performed using an Agi- lent 1200 capillary-HPLC system (Germany) coupled to a HCT mass-spectrometer (Bruker, Bremen, Germany) with an ...
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Determining the degradation efficiency and mechanisms of ethyl violet using HPLC-PDA-ESI-MS and GC-MS
... Degradation mechanisms of EV Most EV • radicals are generated directly from the redox re- action between Zn-foil and surface-adsorbed EV dye. The attack by H 2 O on the ethyl group of diethylamine resulted in ...
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Simultaneous Determination of Ten Endocrine Hormone Disrupters in Water Using SPE/LC (ESI)MS MS
... Two transitions were also established, one for quanti- fication (MRM1) and one for qualification (MRM2), after optimization of the triple quadrupole conditions, using argon as collision gas. This operation was ...
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SCREENING FOR DRUGS IN SERUM AND URINE BY LC/ESI/CID-MS AND MS/MS WITH LIBRARY SEARCHING *
... chemicals using a Perkin-Elmer/SCIEX ionspray source has been investigated with the aim of achieving high reproducibility for setting-up mass spectra libraries with different degrees of fragmentation ...molecular ...
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PICOGRAM LEVEL QUANTIFICATION OF VARENICLINE IN HUMAN PLASMA SAMPLES BY LC–ESI MS/MS
... plasma using Varenicline -D4 as Internal Standard ...protonated ions were formed by a turbo ionspray in a positive mode was used to detect analyte and internal standard ...
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Investigating the Structural Compaction of Biomolecules Upon Transition to the Gas-Phase Using ESI-TWIMS-MS
... 2PCV (5 mM cacodylate, 50 mM NaCl, and 0.1 mM EDTA [48]), and that this may have affected the CCS values obtained from the three biophysical techniques. Although beyond the scope of this study, a systematic analysis of ...
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Investigating the structural compaction of biomolecules upon transition to the gas-phase using ESI-TWIMS-MS
... 2PCV (5 mM cacodylate, 50 mM NaCl, and 0.1 mM EDTA [48]), and that this may have affected the CCS values obtained from the three biophysical techniques. Although beyond the scope of this study, a systematic analysis of ...
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Screening natural libraries of human milk oligosaccharides against lectins using CaR ESI MS
... representative ESI mass spectrum acquired in negative ion mode for an aqueous ammonium acetate solution (40 mM, pH ...BabA ions, at charge states -11 to -14, were ...complex ions were not well ...
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DESI MS Imaging Using SONAR: A Novel Method to Image Precursor Ions and Fragment Ions for Molecular Identification in a Single Experiment
... precursor ions with fragment ions ■ Introduction Mass spectrometry imaging (MSI) allows the correlation of spatial and chemical information directly from biological ...the ESI-like spectra, and also ...
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Determination of pharmaceuticals in freshwater sediments using ultrasonic-assisted extraction with SPE clean-up and HPLC-DAD or LC-ESI-MS/MS detection
... and ESI source temperature, capillary and cone voltage and flow rate of desolvation gas were optimized to obtain the highest intensity of precursor molecules of the six ...fragment ions obtained. Precursor ...
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Exploring the proteome by CE-ESI-MS
... The success of the genome sequencing projects in the late 90’s and early 2000’s boosted enthusiasm and hope for easy implementation of the genomic approach to other levels of biological regulation[1]. The totality of an ...
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Characterization of Intact Proteins by ESI-LC/MS
... – for removal of detergents and other components not compatible with mass spec or protein digestion. compatible with mass spec or protein digestion – Methods (See appendix for protocols[r] ...
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Estimation of Ribociclib in Human Plasma Samples by LC ESI MS/MS
... Q3 ions Chromatographic Conditions For chromatographic resolution 5 mM ammonium formate: acetonitirle (10:90 v/v) was used as mobile phase and analytical column Zorbax SB-C18, ...
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Identification of b-/y-ions in MS/MS spectra using a two stage neural network
... De novo peptide sequencing addresses the ill-posed pro- blem of deriving the correct peptide sequence corre- sponding to a tandem MS spectrum. The first critical step in this problem is that of identifying those ...
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Validation of a UHPLC-ESI-MS/MS Method for Anthocyanidin Quantification in Potato Tubers
... The development of bioanalytical methods has be- come more and more challenging over the past years due to the very demanding requirements in terms of method reliability, sensitivity, speed of analysis, and sample ...
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A VALIDATED ANALYTICAL METHOD FOR QUANTIFICATION OF CRISABOROLE IN MATRICES BY UPLC ESI MS/MS
... MS method for quantification of Crisaborole in aqueous and human plasma has been successfully developed and validated. A simple and inexpensive liquid-liquid extraction procedure and an isocratic chromatography ...
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Measurement of methionine level with the LC-ESI-MS/MS method in schizophrenic patients
... The retention time refers to the time that passes between the sample’s first entry into the column and the highest detector response following its exit from the column. While every substance has a different retention ...
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Investigation of Biological Membranes by NMR and ESI-MS Methodologies
... an ESI-MS apparatus with its main parts: a nebulizing source, an ion beam guide and a mass analyzer During standard electrospray ionization [43], the sample is dissolved in a polar, volatile solvent and ...
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Phytochemical Study of Nothapodytes foetida by LC-ESI-MS
... foetida using reverse-phase high performance liquid chromatography coupled with on-line photodiode array detection and electrospray-ionization tandem mass spectrometry without isolation and purification of these ...
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