Real-time and conventional RT-PCR
Development of one step SYBR Green real time RT PCR for quantifying bovine viral diarrhea virus type 1 and its comparison with conventional RT PCR
... as conventional reverse transcription-polymerase chain reaction (RT-PCR) [10-12] and nested PCR [13] for the detection of BVDV have been ...usually time-consuming and the results are ...
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External Quality Assessment for Enterovirus 71 and Coxsackievirus A16 Detection by Reverse Transcription PCR Using Armored RNA as a Virus Surrogate
... commercial real-time RT-PCR kits (PCR assays A to E), one triplex commercial real-time RT- PCR assay (PCR assay F), and one in-house ...
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Real-time PCR and real-time RT-PCR applications in food labelling and gene expression studies
... Conventional RT-PCR was widely used for gene-specific mRNA quantification during the early days of the introduction of real-time ...endpoint RT-PCR ...gene-specific ...
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Broadly Reactive and Highly Sensitive Assay for Norwalk Like Viruses Based on Real Time Quantitative Reverse Transcription PCR
... all RT-PCR ...EM. Conventional RT-PCR methods did not detect other RNA viruses, such as hepatitis A virus, hep- atitis E virus, poliovirus, Sapporo-like viruses, and Astro virus (data ...
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Comparison of SYBR green I real time RT PCR with conventional agarose gel based RT PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco
... I real-time RT-PCR targeting the IBV N gene would also be positive using a one-step RT-PCR target- ing the IBV S1 ...one-step RT-PCR. The obtained RT- ...
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Identification of Dengue Virus in Respiratory Specimens from a Patient Who Had Recently Traveled from a Region Where Dengue Virus Infection Is Endemic
... the conventional RT-PCR, sequencing, and real-time RT- PCR confirmed and clarified the subtype of dengue virus that was ...by conventional RT-PCR, ...
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Evaluation and Validation of Real Time Reverse Transcription PCR Assay Using the LightCycler System for Detection and Quantitation of Norovirus
... load. Real-time quantitative RT-PCR systems are slowly re- placing conventional RT-PCR assays for the detection and quantification of RNA viruses in research and ...
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Superiority of Digital Reverse Transcription PCR (RT PCR) over Real Time RT PCR for Quantitation of Highly Divergent Human Rhinoviruses
... by conventional PCR using the type-specific T7 polymerase promoter-labeled forward primer and type-specific reverse ...for RT-qPCR and 1e2 to 1e6 copies/reaction for RT-dPCR) were amplified using ...
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Development and implementation of the quality control panel of RT PCR and real time RT PCR for avian influenza A (H5N1) surveillance network in mainland China
... as RT-PCR and rRT-PCR with high sensitivity and specifi- city have had indispensable effect on laboratory rapid diagnosis of ...applied PCR detec- tion system. However, PCR as a ...
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Quantitative real-time RT-PCR of CD24 mRNA in the detection of prostate cancer
... Established conventional prognostic markers are prostate-specific antigen (PSA) [2], the tumor stage and grade [3], the patient's age [4], and the presence of resid- ual tumor(s) ...
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Highly Sensitive Methods Based on Seminested Real Time Reverse Transcription PCR for Quantitation of Human Immunodeficiency Virus Type 1 Unspliced and Multiply Spliced RNA and Proviral DNA
... seminested real-time reverse transcription-PCR (RT-PCR) that combines the accuracy and precision of real-time PCR and the sensitivity of nested ...the ...
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Optimization of a Combined Human Parechovirus Enterovirus Real Time Reverse Transcription PCR Assay and Evaluation of a New Parechovirus 3 Specific Assay for Cerebrospinal Fluid Specimen Testing
... is time-consuming, laborious, and less sensitive than molecular approaches for CSF pathogen ...few real- time reverse transcription-PCR (RT-PCR) assays have been devel- oped for ...
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Detection of asymptomatic carriers of malaria in Kohat district of Pakistan
... (RDT), real time polymerase chain reaction (RT-PCR) and RT-PCR followed by endpoint fluorometry (EPF) for detection of malaria in asymptomatic immediate family members of ...
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Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus
... This real-time PCR, which could be completed in 1 h, was a rapid method allowing the fast detection and confirmation of dengue viruses in clinical ...
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Over expression of lysophosphatidic acid receptor 2 in human invasive ductal carcinoma
... USA). Real-time PCR reactions were conducted in an ABI PRISM 7000 (Perkin-Elmer/ Applied Biosystems, Foster, CA, USA) using SYBR Green I (Perkin-Elmer) with the following profile: one step at 50°C ...
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Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis
... including RT-PCR and mPCR/ RLB, do not depend on the presence of viable or grow- ing bacteria, and thus are suitable to the detection of pathogens that cannot be cultured readily by routine methods, or that ...
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Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
... one-step real-time RT-PCR assay for the detection of Middle East respiratory syndrome coronavirus (MERS- CoV) to investigate the potential of a hot start RT for improved ...
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Comparison and Evaluation of Real Time PCR, Real Time Nucleic Acid Sequence Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae
... Primers and probes for Mycoplasma real-time PCR. Using criteria required for the design of molecular-beacon assays, primer and molecular-beacon sequences were selected from a P1 cytadhesin gene ...
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Development and Validation of a Quantitative PCR Assay for Diagnosis of Scedosporiosis
... Two real-time PCR (RT-PCR) assays for the diagnosis of these species were developed and validated for the classification of clinical strains and for the detection of DNA in clinical ...
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Universal conventional and real time PCR diagnosis tools for Sarcoptes scabiei
... The specificity of the generic primers for the universal diagnosis of S. scabiei infection was evaluated using ref- erence samples of S. scabiei preserved in the authors’ mite collection, as well as heterologous samples ...
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