real-time polymerase chain reaction
Diagnosis by real time polymerase chain reaction of pathogens and antimicrobial resistance genes in bone marrow transplant patients with bloodstream infections
... multiplex real- time polymerase chain reaction (PCR) detection of Gram-positive and Gram-negative bacteria have been previously described, as well as species-specific TaqMan probes for ...
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Development of real time polymerase chain reaction for detection of Salmonella typhimurium and Salmonella enteritidis in fish
... and real-time PCR, and detection based on DNA or ...products. Real time PCR is a more recently developed technique compared to endpoint ...a real time PCR method for Salmonella ...
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Performance evaluation of optimal real-time polymerase chain reaction achieved with reduced voltage
... the time needed to reach the target temperature, we performed PCR by reducing the area of the heater pattern to one-quarter or less of the conventional ...in time to reach the target temperature would have ...
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Clustering techniques for DNA computing readout method based on real-time polymerase chain reaction
... based real-time PCR for reading out DNA solution that encodes the Hamiltonian ...the real-time PCR can be distinguished as either “YES” or “NO” ...
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Rapid Detection of Shigella flexneri on Egg Samples by the Real Time Polymerase Chain Reaction Method
... Based on the data in Fig. 4, there is a specific DNA band of Shigella flexneri with an amplicon size of 188 bp in all conditions of the temperature of 57°C-61°C, so that the product has been successfully amplified. This ...
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Expression of Mammaglobin and Carcinoembryonic Antigen in Peripheral Blood of Patients with Breast Cancer Using Real Time Polymerase Chain Reaction
... [16] Khare, P.D., Shao-Xi, L., Kuroki, M., Hirose, Y., Arakawa, F., Nakamura, K., et al . (2001) Specifically Targeted Killing of Carcinoembryonic Antigen (CEA)-Expressing Cells by a Retroviral Vector Displaying ...
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Real-Time Polymerase Chain Reaction for the Rapid Detection of Group B Streptococcal Colonization in Neonates
... and real-time ...by real-time PCR on the incidence of invasive GBS disease could not be ...the real-time PCR technique in neonates and as a direct approach to neonatal manage- ...
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Nasal swab samples and real time polymerase chain reaction assays in community based, longitudinal studies of respiratory viruses: the importance of sample integrity and quality control
... Between September 2010 and July 2012, 152 infants were recruited into the study. All participants lived within the greater Brisbane metropolitan area and none were from rural communities. One-hundred and twenty- five ...
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A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification
... RT reaction. In conventional RT-PCR, chemicals such as TRIzol help denature the capsid protein and release the viral RNA. A high temperature also facilitates release of viral RNA. As an example, pretreatment at ...
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New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... The first step towards analysing microbial gene expression requires a quantitative extraction of RNA. This step has proven to be highly problematic for environmental matrices, due to compounded inefficiencies in ...
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Effects of Stress on Mouse β Defensin 3 Expression in the Upper Digestive Mucosa
... Total RNA was isolated from each tissue sample by using ISOGEN Reagent (Nippon Gene Co. Ltd., Toyama, Japan) according to the manufacturer’s instructions. RNA quality was judged from the ribosomal RNA pattern after ...
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Seven-microRNA panel for lung adenocarcinoma early diagnosis in patients presenting with ground-glass nodules
... Methods: To identify potential miRNAs of interest, we analyzed the miRNA expression profile of tumor and adjacent non-para-tumor tissue in three participants by next-generation sequencing (NGS). We then assessed the ...
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Improved detection of respiratory viruses in pediatric outpatients with acute respiratory illness by real time PCR using nasopharyngeal flocked swabs
... James Nokes Article Title: Increased detection of respiratory viruses in paediatric outpatients with acute respiratory illness by real-time polymerase chain reaction using nasopharyngeal[r] ...
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Regeneration and reprogramming compared
... quantitative real time polymerase chain reaction (qPCR) of regenerating and non-regenerating tissue compared to an embryonic, pluripotent reference cell type, analysis of the blastema ...
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Local expression of matrix metalloproteinases, cathepsins, and their inhibitors during the development of murine antigen induced arthritis
... As in RA, breakdown of articular cartilage and bone in AIA results from the overexpression of proteinases and deregu- lation of the balance between proteinases and their inhibi- tors. We investigated the expression ...
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Optimizing polymerase chain reaction testing for the diagnosis of pertussis: current perspectives
... infection. Real-time polymerase chain reaction (RT-PCR) is an important tool for timely diagnosis of pertussis and is more sensitive than ...
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PubMedCentral-PMC4931989.pdf
... quantitative real-time polymerase chain reaction (PCR) with these upregulated miRNAs from the miRNA microarray, and 8 miRNAs showed significantly increased expression in A2780 ovarian ...
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<p>Effects of dynein light chain Tctex-type 3 on the biological behavior of ovarian cancer</p>
... quantitative real-time polymerase chain reaction and Western blotting respectively, and the proliferation, apoptosis, migration and invasion of SKOV3 cells after DYNLT3 over-expression ...
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Long noncoding RNA CASC2 predicts the prognosis of glioma patients and functions as a suppressor for gliomas by suppressing Wnt/β-catenin signaling pathway
... Notes: (A–C) β-catenin, cyclin-D1, and c-Myc were upregulated in glioma tissues compared with adjacent normal brain tissues, as found by qrT-Pcr analysis. (D) Overexpression of casc2 suppressed the protein expression of ...
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Asymmetric effects of native and exotic invasive shrubs on ecology of the West Nile virus vector Culex pipiens (Diptera: Culicidae)
... development time, and adult body ...quantitative real-time polymerase chain reaction to test the hypothesis that variation in emergence, development, and oviposition site ...
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