real-time polymerase chain reaction analysis
Long noncoding RNA CASC2 predicts the prognosis of glioma patients and functions as a suppressor for gliomas by suppressing Wnt/β-catenin signaling pathway
... quantitative real-time polymerase chain reaction analysis, our data showed that CASC2 expression was significantly downregulated in glioma tissues and cell lines (U87 and U251) ...
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Development of real time polymerase chain reaction for detection of Salmonella typhimurium and Salmonella enteritidis in fish
... and real-time PCR, and detection based on DNA or ...products. Real time PCR is a more recently developed technique compared to endpoint ...a real time PCR method for Salmonella ...
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Establishment of real time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene
... DNA polymerase(reverse ...by real-time RT-PCR should be normalized by comparison with transcript abundances of reference genes ...and polymerase chain reaction(PCR) reactions ...
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The prognostic values of the expression of Vimentin, TP53, and Podoplanin in patients with cervical cancer
... Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis were performed to determine the messenger RNA and protein expression levels of Vimentin, ...
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Transforming growth factor activating kinase 1 regulates extracellular matrix degrading enzymes and pain related molecule expression following tumor necrosis factor α stimulation of synovial cells: an in vitro study
... h. Real-time polymerase chain reaction (PCR) analysis was used to monitor expression of mRNA of the extracellular matrix degrading enzymes matrix metalloproteinase-3 (MMP-3) and ...
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Real Time PCR and Real Time RT PCR applications in food labelling and gene expression studies
... Abstract: Polymerase chain reaction (PCR) as a scientific invention, has revolutionized molecular biology and led to real-time PCR and later, real-time reverse ...
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MOLECULAR DETECTION OF INSERTION SEQUENCE 6110 OF MYCOBACTERIUM TUBERCULOSIS IN PATIENTS WITH PULMONARY TUBERCULOSIS AND TUBERCULOUS PLEURITIS IN ANBAR GOVERNORATE, WEST OF IRAQ
... and real time polymerase chain reaction in the diagnosis of pulmonary tuberculosis caused by Mycobacterium tuberculosis in sputum and blood samples using 123 bp gene of repetitive ...
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Performance evaluation of optimal real-time polymerase chain reaction achieved with reduced voltage
... Background: Polymerase chain reaction (PCR) is used in nucleic acid tests of infec‑ tious diseases in point‑of‑care ...demonstrated real‑time PCR that uses a micro‑PCR chip made of ...
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Cloning of a muscle specific calpain from the American lobster Homarus americanus: expression associated with muscle atrophy and restoration during moulting
... Here we report the cloning of a crustacean calpain gene with features that distinguish it from calpains isolated from other species. The cDNA encodes a non-EF-hand calpain with a unique N-terminal sequence and an ...
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Diagnosis by real time polymerase chain reaction of pathogens and antimicrobial resistance genes in bone marrow transplant patients with bloodstream infections
... statistical analysis were performed using SSPS version ...versus real-time PCR results were evaluated by χ 2 ...and real-time PCR ...
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Effects of Stress on Mouse β Defensin 3 Expression in the Upper Digestive Mucosa
... used real-time polymerase chain reaction, in situ hybridization, and immu- nohistochemistry to investigate the effects of stress and glucocorticoid administra- tion on mouse ...
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Regeneration and reprogramming compared
... quantitative real time polymerase chain reaction (qPCR) of regenerating and non-regenerating tissue compared to an embryonic, pluripotent reference cell type, analysis of the ...
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Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.
... length analysis have been published (Miura-Ochiai R et al 2007; Ebner K et al 2006; Blyn LB et al 2007; Lin B et al 2004; Lu B et al ...perform real-time AdV assay for clinical monitoring or to ...
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A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3', 5'-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium
... inhibitor. Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that transcription of most LiP and MnP isozyme genes was statistically ...
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Seven-microRNA panel for lung adenocarcinoma early diagnosis in patients presenting with ground-glass nodules
... non-tumor analysis by NGS was assayed by quantitative real-time polymerase chain reaction (qRT- PCR) in 73 lung adenocarcinoma patients who presented with ...
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New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... expression analysis using RT-qPCR entails the synthesis of cDNA by the reverse transcriptase (RT) enzyme, followed by an exponential amplification of the template by DNA polymerase, any chemical moiety, ...
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PCR Diagnosis of Invasive Candidiasis: Systematic Review and Meta Analysis
... (multiplex real-time PCR). A subgroup analysis based on these variables in addition to those observed in the previous analysis (use of whole-blood samples and rRNA or P450 target genes) ...
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Analysis of striatal transcriptome in mice overexpressing human wild-type alpha-synuclein supports synaptic dysfunction and suggests mechanisms of neuroprotection for striatal neurons
... 0.2 mM NaVa, 10 U/ml aprotinine, 20 U/ml DNAse I, 2 ug/ml RNAse, 1% mercaptoethanol) and centrifuged for 2 min, at 12,000 × g, at 4°C; and the soluble fraction was collected and frozen until used. The insoluble pellet ...
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Prognostic implications of Kindlin proteins in human osteosarcoma
... survival analysis, all 100 osteosarcoma patients received ...the time interval from the date of diagnosis at our center to the date of death or the last ...the time interval from diagnosis at our ...
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Sulfur mustard induces expression of metallothionein-1A in human airway epithelial cells
... Quantitative real-time reverse transcriptase polymerase chain reaction was then performed in a Rotor-Gene RG 3000 (Corbett Research, Mortlake, ...Quantitative polymerase ...
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