Real-time qRT-PCR
The Spike Protein of Murine Coronavirus Regulates Viral Genome Transport from the Cell Surface to the Endoplasmic Reticulum during Infection
... endosome. Real-time qRT-PCR was then per- formed to quantify the viral genomic RNAs in the ER by use of a primer pair corresponding to the most-5 ⬘ -end 177 nucle- otides of the viral genome ...
11
RNA editing analysis of ATP synthase genes in the cotton cytoplasmic male sterile line H276A
... editing. Real-time qRT-PCR data showed that the atp1, atp6, atp8, and atp9 genes had substantially lower expression levels in H276A compared with those in ...
9
Seven-microRNA panel for lung adenocarcinoma early diagnosis in patients presenting with ground-glass nodules
... quantitative real-time polymerase chain reaction ...When qRT-PCR was performed for the 23 miRNAs identified by NGS in the pilot stage, seven were found to have statistically significant ...
12
Short-term pacing in the mouse alters cardiac expression of connexin43
... Quantitative Real-Time PCR (qRT-PCR) Demon- strates Decreased Cx43 mRNA Levels in Paced Hearts.. We found that pacing at the epicardial surface of the RV using the subdiaphragmatic appr[r] ...
12
Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling
... Step 4: Selection and validation of miRNA biomarkers In this step, large numbers of samples (50 to 200) are separately used for validating the selected miRNAs in the profiling step. Around 10 to 20 miRNAs with the ...
9
High level expression of the anti retroviral protein APOBEC3G is induced by influenza A virus but does not confer antiviral activity
... quantitative real time PCR ...The qRT-PRC-program is limited to 40 cycles; due to that fact that CT-values >40 indicate unde- tectable amounts of ...the time points ...
9
Analysis of striatal transcriptome in mice overexpressing human wild-type alpha-synuclein supports synaptic dysfunction and suggests mechanisms of neuroprotection for striatal neurons
... Quantitative real-time PCR (qRT-PCR) analysis was per- formed with both pooled striatal RNA samples used in the microarray analysis and individual striatal RNA sam- ples from 3 male ...
16
IDH1 mutation detection by droplet digital PCR in glioma
... digital PCR (ddPCR) technique generates a large amount of nanoliter-sized droplets, each of which carries out a PCR reaction on one ...quantitative real-time PCR ...with qRT- ...
10
Characterization of hepatitis B and delta coinfection in Israel
... In a cohort of HBV/HDV coinfected n=58 and HBV monoinfected n=27 patients, quantitative real-time PCR qRT-PCR and sequencing were performed to determine viral loads, genotypes and hepati[r] ...
8
Establishment and Application of Digital RT PCR Assay for Detection of Avian Influenza Virus H9 Subtype
... digital PCR tests, droplets and chip. Unlike traditional quantitative PCR techniques, dPCR is independent of the circula- tory threshold (CT) of the amplification curve and is not affected by amplifica- ...
9
Overexpression of OsbHLH107, a member of the basic helix-loop-helix transcription factor family, enhances grain size in rice (Oryza sativa L.)
... Quantitative real-time PCR (qRT-PCR) analysis further showed that the expression levels of genes associated with the cell cycle in the young panicles of the lgs1 were higher than those ...
12
The prognostic values of the expression of Vimentin, TP53, and Podoplanin in patients with cervical cancer
... Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis were performed to determine the messenger RNA and protein expression levels of Vimentin, TP53, and ...
12
Original Article All-trans retinoic acid in combination with collagen IV induces the differentiation of mouse embryonic stem cells into smooth muscle cells
... vivo. Real-time Quantitative PCR (qRT-PCR), Western blot and immunofluorescence staining were used to detect α-smooth muscle actin (α-SMA) and smooth muscle myosin heavy chain (SM-MHC) ...
7
Improved Single Copy Assays for Quantification of Persistent HIV 1 Viremia in Patients on Suppressive Antiretroviral Therapy
... For real-time PCR, the LightCycler 480 Probes Master ready-made master mix was used at a ...for real-time PCR were 95°C for 5 min, followed by 95°C for 10 s and 60°C for 1 min, ...
8
miR-486-5p suppresses prostate cancer metastasis by targeting Snail and regulating epithelial–mesenchymal transition
... Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) results demon- strated that the metastatic C-4 cell line showed significantly decreased expression of ...
6
Clinicopathological and prognostic significance of long noncoding RNA MALAT1 in human cancers: a review and meta-analysis
... 1; qRT-PCR: quantitative real-time polymerase chain reaction; ISH: in situ hybridization; HWE: hardy–weinberg equilibrium; HR: hazard ratio; OS: overall survival; DFS: disease-free survival; ...
12
Ruxolitinib/nilotinib cotreatment inhibits leukemia propagating cells in Philadelphia chromosome positive ALL
... sequencing; qRT‑PCR: real‑time quantitative polymerase chain reaction; JAK: Janus kinase; Rux‑ olitinib: JAK1/JAK2 inhibitor; BCR–ABL: breakpoint cluster region, Abelson; CML: chronic myeloid ...
13
Original Article MicroRNA-21 and microRNA-146a identification in stool and its clinical significance in colorectal neoplasms
... quantitate real-time polymerase chain reaction (qRT-PCR) from the tissues of colorectal carcinoma (CRC), colorectal adenoma (CRA) and healthy controls as well as their matched stool ...
9
Angiogenesis and vasculogenic mimicry are inhibited by 8-Br-cAMP through activation of the cAMP/PKA pathway in colorectal cancer
... for qRT-PCR were designed using online tools available at the Primer3 ...website. qRT-PCR was performed on a StepOnePlus Real-Time PCR system (Thermo Fisher Scientific) ...
10
Original Article Differential expressions of BMPR1α, ACTN4α and FABP7 in Hirschsprung disease
... Abstract: Hirschsprung disease (HSCR) is characterized by the absence of intramural ganglion cells in the nerve plex- uses of the distal gut. Recent studies have shown that the bone morphogenetic protein receptor-type IA ...
7