recombinant baculovirus
A novel recombinant baculovirus overexpressing a Bacillus thuringiensis Cry1Ab toxin enhances insecticidal activity
... type baculovirus can be used to express and study the properties of the insecticidal Bacillus thuringiensis protein yielding infor- mation relevant to an understanding of the molecular biology of this protein as ...
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Targeting of an expressed neurotoxin by its recombinant baculovirus
... a recombinant baculovirus expressing the green fluorescence protein (Barrett et ...the recombinant virus and its expressed neurotoxin follows from the fact that: (i) the tracheal system is ...
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Chromosomal Integration of Transduced Recombinant Baculovirus DNA in Mammalian Cells
... of recombinant baculoviruses to transduce mammalian cells at high ...of baculovirus as a mammalian gene delivery system, we examined the status of transduced DNA stably maintained in Chinese hamster ovary ...
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Assembly of double-shelled, viruslike particles of bluetongue virus by the simultaneous expression of four structural proteins.
... Coexpression of the two major core proteins VP3 and VP7 in insect cells by a multiple recombinant baculovirus results in the synthesis of CLPs of the same size, appearance, and stoichiom[r] ...
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Innate Immune Response Induced by Baculovirus Attenuates Transgene Expression in Mammalian Cells
... by recombinant baculovirus in ...that baculovirus induced proinflammatory cyto- kines and type I IFNs in MEFs through the TLR-dependent and TLR-independent pathways, respectively ...combinant ...
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In Vitro Study of the Effects of Precore and Lamivudine-Resistant Mutations on Hepatitis B Virus Replication
... Several previous studies have reported that the rtM204I mutant replicates in vitro with a lower efficiency than that of wt HBV (17, 18, 29, 36). The data we report here disagree with these findings for early time points ...
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Baculoviral Expression of Influenza A Virus (H1N1 New Caledonia) Neuraminidase in Insect Cells
... with recombinant baculovirus were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoreses (SDS-PAGE) and stained with coomassie brilliant ...
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Generation of mini-Tn7 transposone by cloning of CPV-VP2 into eukaryotic expression cassette, in E. coli
... Bac-to-Bac Baculovirus Expression System facilitates rapid and efficient generation of recombinant ...Bac-to-Bac Baculovirus Expression System takes advantage of the site-specific transposition ...
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Functionality and Cell Anchorage Dependence of the African Swine Fever Virus Gene A179L, a Viralbcl-2 Homolog, in Insect Cells
... on recombinant baculovirus growth was also investigated ...the baculovirus replication phenotype in Sf9 ...both recombinant viruses ...to baculovirus infection at late time ...
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Development of Neuraminidase Subtype-Specific Reference Antisera by Recombinant Protein Expressed in Baculovirus
... a recombinant baculovirus. The recombinant NA (rNA) proteins were expressed in Sf9 insect cells and inoculated intramuscularly into specific-pathogen-free chickens with the ISA70 ...
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A Cholesterol Recognition Amino Acid Consensus Domain in GP64 Fusion Protein Facilitates Anchoring of Baculovirus to Mammalian Cells
... the baculovirus genetic material from reaching the host cell ...the baculovirus genome were conducted with HEK293T cells expressing the human histone H2 (H2-GFP), used to identify the ...
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Recombinant virus vaccine for bluetongue disease in sheep.
... Bluetongue virus tubules made in insect cells by recombinant baculovirus: expression of NS1 gene of bluetongue virus serotype 10.. Structure of the bluetongue virus capsid.[r] ...
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Angiopoietin-1-expressing adipose stem cells genetically modified with baculovirus nanocomplex: investigation in rat heart with acute infarction
... using recombinant baculovirus complexed with cell penetrating transactivating transcriptional activator TAT peptide/deoxyribonucleic acid nano- particles (Bac-NP), through ionic interactions, was ...
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Bluetongue virus tubules made in insect cells by recombinant baculoviruses: expression of the NS1 gene of bluetongue virus serotype 10.
... To demonstrate that BTV-10 NS1 protein was synthesized in recombinant baculovirus-infected cells, protein extracts were prepared from insect cells infected with the recombinant virus AcB[r] ...
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Quantification of systemic and local immune responses to individual rotavirus proteins during rotavirus infection in mice
... using recombinant baculovirus-expressed ...of recombinant rotavirus structural proteins have indicated that they do not always assume the conforma- tions present on the ...binant baculovirus ...
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Assembly and Intracellular Localization of the Bluetongue Virus Core Protein VP3
... To make certain that these patterns did not represent any artifacts contributed due to the presence of EGFP, Sf9 cells infected with recombinant baculovirus expressing EGFP-VP3 were immunolabeled with an ...
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Isolation, characterization and expression of cDNAs encoding human and marmoset cytochrome P450's
... Sf9 cells contain endogenous NADPH-cytochrome P450 reductase which makes expressed P450 directly active in Sf9 cells microsomes. Expression and purification of functional CYP2A6 was achieved in Sf9 cells using ...
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Construction of Recombinant Bacmid DNA Encoding Newcastle Disease Virus (NDV) Fusion Protein Gene
... The recombinant baculovirus and insect cell expression system provides high levels of recombinant proteins that undergo post- translational modifications like glycosylation ...containing ...
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Site-specific glycosylation of the human cytomegalovirus tegument basic phosphoprotein (UL32) at serine 921 and serine 952.
... DISCUSSION The primary findings of this report are that i the full-length BPP from HCMV strain AD169 can be overexpressed as a recombinant baculovirus in Sf9 insect cells, ii baculovirus[r] ...
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Construction of a Recombinant Bacmid DNA to Express Influenza Virus Matrix Protein1 (M1) in Insect Cell Line
... The recombinant baculovirus and insect cell expression system provides high levels of recombinant protein that undergoes post- translational modification like glycosylation (7, ...M1 ...
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