Recombinant Protein
Expanding the recombinant protein quality in Lactococcus lactis
... of recombinant proteins of biomedical interest, but conformational stress responses impose severe bottlenecks that impair the production of soluble, proteolytically stable versions of many protein ...for ...
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Protein Body Biogenesis and Utility in Recombinant Protein Production in Nicotiana benthamiana
... for recombinant protein production and offers several advantages including flexibility and rapid scalability for protein production (D'Aoust et ...improve protein yield, prevent proteolysis, ...
153
Evaluation of the Recombinant Protein TpF1 of Treponema pallidum for Serodiagnosis of Syphilis
... expressed recombinant protein TpF1 in E. coli, and purified the recombinant protein by His tag-based affinity chromatography under native ...expressed recombinant protein was ...
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Estimation of the Amount of Recombinant Protein A Secretion Using Fuzzy Regression
... of recombinant protein is a wide production method which is currently ...extracellular protein is very desirable so that it can reduce the complexity of lower part processes and can help the ...
10
Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris
... of recombinant protein expression, we suggested a potential role of ergosterol or an intermediate during the protein secretion ...influence protein secretion, its biosynthesis was perturbed by ...
15
Trends in recombinant protein use in animal production
... and protein engineering techniques have led to a significant progress in animal produc- tion and it is starting to have a commercial impact in this ...that recombinant protein production ...of ...
17
Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield
... binant protein remains a major bottleneck in contemporary bioscience ...eukaryotic protein production experiments have relied on varying either promoter and fusion tag combina- tions in expression ...
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Whole synthetic pathway engineering of recombinant protein production
... from protein biomanufacturing systems is a function of total host cell biomass synthetic capacity and recombinant protein production per unit cell ...the protein synthetic pathway and the use ...
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Potential Serological Use of a Recombinant Protein That Is a Replica of a Mycobacterium tuberculosis Protein Found in the Urine of Infected Mice
... tuberculosis recombinant proteins has revived interest in the serological diagnosis of ...tuberculosis protein that is a promising candidate for increasing the sensitivity of the serological tests, in ...
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Suppression of inflammation and tissue damage by a hookworm recombinant protein in experimental colitis
... the recombinant protein anti-in fl ammatory protein (AIP)-1, secreted in abundance by hookworms within the intestinal mucosa, in experimental ...
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A GFP strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis
... In this study we have developed a new method for the efficient screening and purication of mycobacterial proteins using GFP and M. smegmatis as a host expression system. Here we have identied that GFP can be used as a ...
9
Genome engineering for improved recombinant protein expression in Escherichia coli
... improved protein folding in vivo using chaperone co-expression ...helps protein folding while chaperones like GroEL encapsulates the nascent polypeptide and prevents inter molecular interactions ...of ...
13
A study of the recovery and purification of a recombinant protein
... of protein is a very simple technique, which is carried out in free solution, and is ideally suited to the initial treatment of large volume feedstreams containing low levels of recombinant ...of ...
248
Control of recombinant protein productivity and quality in mammalian cell culture
... required recombinant protein are favoured since natural copy numbers of the vector in the host genome are likely to remain ...desired recombinant protein gene within a chromosome then ...
234
Pichia pastoris: a workhorse for recombinant protein production
... BIOPROCESS OPERATIONS AND OPTIMIZATION Bioprocess parameters including medium composition, pH, temperature, aeration rate, induction and feeding strategies are of utmost importance since it affects directly the ...
10
Signal peptides for recombinant protein secretion in bacterial expression systems
... respective protein translocases in the cytoplasmic membrane ...mature protein part to allow its efficient interaction with posttranslationally interact- ing proteins (such as SecB) and, by this means, help ...
10
Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification
... eral types of proteins were found to attach on the surface of in vivo PHA granules, such as PHA synthase (PhaC), PHA depolymerase (PhaZ), granule associated protein (PhaP) and repressor protein (PhaR) ...
6
Evaluation of Hepatitis C Virus Glycoprotein E2 for Vaccine Design: an Endoplasmic Reticulum-Retained Recombinant Protein Is Superior to Secreted Recombinant Protein and DNA-Based Vaccine Candidates
... of recombinant E2 protein was evaluated by both the capacity to bind its putative receptor CD81 on human cells and the ability to elicit antibodies that inhibited this binding (NOB ...comparing ...
8
Tuning recombinant protein expression to match secretion capacity
... range protein standard curve ...tify protein amounts by western ...scFv protein was detected with mouse monoclonal Anti-His antibody (Pierce, 1:3000 in PBS 5% milk) and IRDye 680RD donkey anti- mouse ...
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Thermal adaptability of Kluyveromyces marxianus in recombinant protein production
... A peculiar trait of this yeast is the capacity to grow within a wide interval of temperatures, ranging from 4°C to 45°C. This feature has been investigated in a study where the adaptive responses at different ...
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