relative quantification
Proteomic analysis of Sauvignon blanc grape skin, pulp and seed and relative quantification of pathogenesis-related proteins
... and quantification of them in grape berries could be of great interest for winemakers to poten- tially reduce their concentrations in juice through managing the extraction during juice pro- ...and ...
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Relative Quantification of N-linked Glycans in Complex Mixtures via Stable Isotope Labeling and Enhanced Analysis by Liquid Chromatography Coupled Online to Mass Spectrometry.
... this quantification strategy, this work has made significant contributions to the field of glycomics and fundamental analytical chemistry including both experimental and practical ...
307
Real time quantitative PCR with SYBR Green I detection for estimating copy numbers of nine drug resistance candidate genes in Plasmodium falciparum
... In this work, the 2 -∆∆Ct method of relative quantification (described in detail in [16]) was adapted to estimate copy numbers in P. falciparum genes. This method allows to estimate gene copy numbers in ...
6
MKRN2 inhibits migration and invasion of non-small-cell lung cancer by negatively regulating the PI3K/Akt pathway
... Fig. 1 Expression of MKRN2 in NSCLC is associated with poor clinical prognosis. a MKRN2 protein level analyzed by immunohistochemistry in (i) alveolar and (ii) normal bronchial epithelial cells, (iii) well-differentiated ...
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Plasma proteome changes associated with refractory anemia and refractory anemia with ringed sideroblasts in patients with myelodysplastic syndrome
... using relative quantification may provide additional information – at least two unique fold change values in two different peptides can prove the presence of PTM(s), which would have influenced the results ...
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In Situ Distribution of Hepatitis C Virus Replicative-Intermediate RNA in Hepatic Tissue and Its Correlation with Liver Disease
... Detection by in situ hybridization of hepatitis C virus positive and negative RNA strands using digoxigenin-labeled cRNA probes in human liver cells.. Relative quantification and mapping[r] ...
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Exon duplications in the ATP7A gene: Frequency and Transcriptional Behaviour
... cDNA was used to detect ATP7A transcript. A FAM or VIC (Applied biosystems proprietary dye) labeled probe and primers for the human GAPDH transcript (part number 4352934E and 4326317E respectively) were used as an ...
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Urinary metabolite profiling identifies novel colonic metabolites and conjugates of phenolics in healthy volunteers
... Box plot of relative quantification of conjugated metabolites of caffeic acid, ferulic acid and isoferulic acid found in urine at different collection times in human volunteers.. Box p[r] ...
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The Chinese soft shelled turtle, Pelodiscus sinensis, excretes urea mainly through the mouth instead of the kidney
... Relative quantification fold-change of the mRNA expression of the putative UT in the anterior roof of the mouth of Pelodiscus sinensis kept in freshwater FW; control, or in terrestrial c[r] ...
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Optimisation of region-specific reference gene selection and relative gene expression analysis methods for pre-clinical trials of Huntington's disease
... the quantification strategy must be considered. Absolute quantification correlates the PCR signal to input copy number using a calibration curve, and is dependent on equivalent amplification efficiencies ...
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Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization
... Protein relative quantification using the Top3 algorithm up-regulated threefold or more and up-regulated in at least two SIV-PS samples and at the same time identified using three or mor[r] ...
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The Development of Analytical Strategies for the Quantitative Proteomic Analysis of Biological Systems Impacting Agriculture and Human Health.
... indicated relative standard deviation of peptide area ratios of around 10%, although the potential for greater error has been reported as a result of deuterium labels producing peak retention times that differ ...
160
Comparison of quantification algorithms for circulating cell-free DNA methylation biomarkers in blood plasma from cancer patients
... For relative quantification, the methylated portion of ccfDNA is referred to the total DNA amount in the sample and can be calculated by the ΔΔCT-method ...
11
Global metabolite profiling based on GC–MS and LC–MS/MS analyses in ABF3-overexpressing soybean with enhanced drought tolerance
... With regard to metabolic variations, environmen- tal factors, e.g., growing season, location, sowing date, and type of abiotic stress, play a larger role than genetic modifications [26–28]. Because those factors may be ...
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A glycine zipper motif mediates the formation of toxic β-amyloid oligomers in vitro and in vivo
... Ab oligomer-bound neurons and dendritic branches were imaged using a Leica (Exton, PA) TCS SP2 laser confocal microscope with laser intensity and signal detection settings held constant to allow for quantitative ...
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Mutations in CECR1 associated with a neutrophil signature in peripheral blood
... the relative abundance of each tar- get transcript was normalized to the expression level of HPRT1 (Hs03929096_g1) and 18S (Hs999999001_s1), and assessed with the Applied Biosystems StepOne Software ...change ...
7
Pinin protects astrocytes from cell death after acute ischemic stroke via maintenance of mitochondrial anti-apoptotic and bioenergetics functions
... Protein samples (50 μg) were electrophoresed on a 7.5% or 12.5% SDS-PAGE gels and then transferred onto a polyvinylidenedifluoride (PVDF) membrane. The mem- brane was incubated overnight at 4 °C with primary anti- ...
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Expression of tumor necrosis factor like weak inducer of apoptosis and fibroblast growth factor inducible 14 in patients with polymyositis and dermatomyositis
... Our further research showed that TWEAK expression at the protein level was remarkably increased in the muscle tissue of PM/DM patients, but TWEAK expres- sion at the mRNA level showed no statistical difference with that ...
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HepG2 Cells Expressing MicroRNA miR-122 Support the Entire Hepatitis C Virus Life Cycle
... presented relative to values for ...for relative transfection efficiency to allow comparison of HCV replication efficiencies independently of trans- fection ...
6
Characterization and use of HapT1-derived homologous tumors as a preclinical model to evaluate therapeutic efficacy of drugs against pancreatic tumor desmoplasia
... Finally, quantification of Ki-67 positive cells showed a significant decrease and increase in the number of proliferative cells in DSF and DSF+Cu treated groups compared with their corresponding controls, ...
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