replication complex
Structural and functional characterization of the poliovirus replication complex.
... Since the completed 36S RNA is found by RNase-gold as well as by in situ hybridization within the rosette of virusinduced vesicles but outside the replication complex per se, it is possi[r] ...
8
Stearoyl Coenzyme A Desaturase 1 Is Associated with Hepatitis C Virus Replication Complex and Regulates Viral Replication
... investigated whether SCD1 could be associated with any viral pro- tein. HEK293T cells were cotransfected with Flag-tagged SCD1 and each of the Myc-tagged HCV proteins, including core, NS3, NS4B, NS5A, and NS5B. Total ...
15
Plum Pox Virus 6K1 Protein Is Required for Viral Replication and Targets the Viral Replication Complex at the Early Stage of Infection
... nated replication vesicles that target the chloroplast for robust viral ...viral replication, suggesting the functional importance of the ma- ture ...viral replication and is an important viral ...
13
Coronavirus Replicase-Reporter Fusions Provide Quantitative Analysis of Replication and Replication Complex Formation
... critical replication enzymes, including nsp12–RNA-dependent RNA polymerase (RdRp) and nsp14-ExoN, requires a ribosomal frameshift between nsp10 and ...track replication complex formation in real time ...
9
An N-Terminal Amphipathic Helix in Hepatitis C Virus (HCV) NS4B Mediates Membrane Association, Correct Localization of Replication Complex Proteins, and HCV RNA Replication
... the replication complex (15, 21), it is tempting to speculate that the AH domain may be involved in the actual formation of these distinct ...the replication complex by enabling, directly or ...
8
Quantitative Analysis of the Hepatitis C Virus Replication Complex
... HCV replication complex therefore appears to represent a more rigid structure due to the com- plete protease and nuclease resistance of in vitro replicase ...
12
Biogenesis of the Semliki Forest Virus RNA Replication Complex
... followed by exposure to BrdUTP for 10 min using lipofectin as a carrier, after which the cells were washed and incubated further for 5 min. The cells were permeabilized and fixed before being double labeled with rabbit ...
12
Recruitment of Brd4 to the Human Papillomavirus Type 16 DNA Replication Complex Is Essential for Replication of Viral DNA
... During the HPV life cycle, E2 binds to its binding sites in HPV16 Ori. Its interaction with E1 enhances E1 binding to the viral Ori and recruits cellular replication factors to the viral DNA replication ...
14
Origin Replication Complex Binding, Nucleosome Depletion Patterns, and a Primary Sequence Motif Can Predict Origins of Replication in a Genome with Epigenetic Centromeres
... DNA replication machinery is highly conserved, yet the definition of exactly what specifies a replication origin dif- fers in different ...origin replication complex, necessary for ...
9
Flavivirus Replication Complex Assembly Revealed by DNAJC14 Functional Mapping
... matic mutant construct representations are shown in Fig. 2A) colocalized with the LD marker adipocyte differentiation-related protein (ADRP, Fig. 2B, left panel, top and middle). The pTM region likely mediated DNAJC14 ...
18
Membrane-Associated Replication Complex in Arbovirus Infection
... 3H-guanosine triphosphate 1.0 for Ci/mmole was obtained from Schwarz BioResearch, 504 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Cytoplasmic extracts of chicken em[r] ...
12
Single Nucleoprotein Residue Modulates Arenavirus Replication Complex Formation
... the replication and tran- scription complexes (RTC) of lymphocytic choriomeningitis virus (LCMV) colocalize with eukaryotic initiation factor 4E (eIF4E) and that eIF4E may participate in the translation of LCMV ...
11
Control of the Replication Complex of Bacteriophage P22
... Replication inhibition of nonvirulent phagea Infection Host Phage Yield of infecting or superinfecting phage per cell _________________________ Virulent 1 Per cent nonvirulent Nonvirulen[r] ...
6
DNA Replication-Initiation Proteins in Eukaryotic Cells
... pre-replication complex is activated by cyclin-dependent kinases (CDKs) and Dbf4p-dependent kinase (DDK) after the cell has passed the restriction point (START in yeast), to form a pre- initiation ...
8
Tyrosine 3 of Poliovirus Terminal Peptide VPg(3B) Has an Essential Function in RNA Replication in the Context of Its Precursor Protein, 3AB
... replication complex assembly is the same in the in vitro trans- lation-RNA replication system as in vivo, it is also known that quantitative differences exist in the ability of the two systems to ...
16
Genomic and Molecular Analyses of the Core DNA Replication Machinery in Plants
... DNA replication proteins” that are integral to the initiation, DNA synthesis, and Okazaki fragment maturation functions of DNA ...DNA replication machinery in ...DNA replication genes exist as small ...
253
Modulation of Hepatitis C Virus Genome Replication by Glycosphingolipids and Four-Phosphate Adaptor Protein 2
... genome replication (17–19, ...genome replication to virus particle produc- ...HCV replication: (i) a mutation in the GLTP domain inhibits HCV RNA synthesis; (ii) inhibition of glucosylceramide ...
20
Comparison of the Transcription and Replication Strategies of Marburg Virus and Ebola Virus by Using Artificial Replication Systems
... artificial replication system estab- lished for MBGV ...virus replication complexes that the amount of input DNA encoding the nucleoprotein could not be increased above a certain level without a decrease in ...
10
Phosphatidylserine-Specific Phospholipase A1 Involved in Hepatitis C Virus Assembly through NS2 Complex Formation
... requires complex protein-protein interactions between HCV proteins and host ...the replication complex (RC) are brought together through the interaction of Core and NS5A, which is very important at ...
11
Complex Dynamic Development of Poliovirus Membranous Replication Complexes
... single-membrane replication-active membranous complexes are being constantly generated throughout the course of ...RNA replication but for other stages in the infectious cycle such as RNA encapsidation or, ...
11