Replication Kinetics
Quantitative estimation of Nipah virus replication kinetics in vitro
... virus replication kinetics were estimated from infection of African green monkey kidney cells (Vero) using the one-step SYBR ® Green I-based quantitative real-time ...
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Impact of Cytokines on Replication in the Thymus of Primary Human Immunodeficiency Virus Type 1 Isolates from Infants
... the replication of HIV primary ...the replication of both X4 and R5 primary isolates in the ...HIV replication in thymocytes is differentially affected by distinct cytokine combinations of IL-2, ...
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Tropism of and Innate Immune Responses to the Novel Human Betacoronavirus Lineage C Virus in Human Ex Vivo Respiratory Organ Cultures
... viral replication and any cytokine induction (data not ...Viral replication kinetics in ex vivo cultures of bronchial (D) and lung (H) biopsy tissue specimens infected with 10 6 ...
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Viral Factors Important for Efficient Replication of Influenza A Viruses in Cells of the Central Nervous System
... efficient replication. Here, we determined the replication kinetics of a sea- sonal, pandemic, zoonotic, and lab-adapted influenza A virus in human neuron-like (SK- N-SH) and astrocyte-like (U87-MG) ...
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Vicriviroc Resistance Decay and Relative Replicative Fitness in HIV-1 Clinical Isolates under Sequential Drug Selection Pressures
... Replication kinetics of HIV-1 recombinants incorporating env genes isolated during VCV and ENF ...the replication kinetics of viruses that contain VCV resistance mutations, we generated re- ...
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Human Immunodeficiency Virus Persistence and Production in T-Cell Development
... Human immunodeficiency virus type 1 (HIV-1) entry in- volves sequential interactions of the viral envelope proteins with CD4 and chemokine receptors, primarily CCR5 or CXCR4 (11, 12, 15). With over 90% of developing ...
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Characterization of human immunodeficiency virus type 1 matrix revertants: effects on virus assembly, Gag processing, and Env incorporation into virions.
... enhanced replication kinetics ob- served with the 6VR/10GR, 6VR/69TI, and 6VR/97KE double mutants relative to that of the 6VR single mutant is accompa- nied by improved virus particle production, HeLa cells ...
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Interferon Resistance of Hepatitis C Virus Genotype 1b: Relationship to Nonstructural 5A Gene Quasispecies Mutations
... replication kinetics. Our results therefore suggest that replica- tion kinetics when treatment is started is a major determinant of the response of HCV-1b to 3 MU of IFN-a three times a ...viral ...
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Construction and Manipulation of an Infectious Clone of the Bovine Herpesvirus 1 Genome Maintained as a Bacterial Artificial Chromosome
... The complete genome of bovine herpesvirus 1 (BoHV-1) strain V155 has been cloned as a bacterial artificial chromosome (BAC). Following electroporation into Escherichia coli strain DH10B, the BoHV-1 BAC was stably ...
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A structural constraint for functional interaction between N terminal and C terminal domains in simian immunodeficiency virus capsid proteins
... viral replication kinetics, HSC-F cells (herpesvirus sai- miri-immortalized macaque T-cell line) [47] were infected with wild-type or mutant SIVs (normalized by RT activity), and virus production was ...
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Cultivation and Serological Characterization of a Human Theiler's-Like Cardiovirus Associated with Diarrheal Disease
... poliovirus replication was observed at a mere 4 h postinfection, whereas viral mRNA from HTCV-UC6 was not visible until day ...slower replication kinetics of HTCV-UC6 relative to poliovirus may be ...
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Insertion of a Multibasic Cleavage Motif into the Hemagglutinin of a Low-Pathogenic Avian Influenza H6N1 Virus Induces a Highly Pathogenic Phenotype
... 48 h after inoculation. Virus titers in MDCK cells were determined by endpoint titration as described previously (10). MDCK cells were inoculated with a 10-fold serial dilution of culture supernatants or tissue ...
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Autographa californica Multiple Nucleopolyhedrovirus ac76 Is Involved in Intranuclear Microvesicle Formation
... The ac76-repair virus showed similar replication kinetics to the wt virus, indicating that the defective phenotype could be rescued by inserting ac76 into the polh locus of the vAcac76-K[r] ...
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The TLR4-TRIF Pathway Protects against H5N1 Influenza Virus Infection
... the replication kinetics of a highly pathogenic H5N1 avian influenza virus (VN1203) in primary mouse lung cells derived from wild-type C57BL/6 mice or knockout mice that lacked the expression of TLR4 ...
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Mutations within the Putative Membrane-Spanning Domain of the Simian Immunodeficiency Virus Transmembrane Glycoprotein Define the Minimal Requirements for Fusion, Incorporation, and Infectivity
... TM189 replication in PBMC was equivalent to that of the wild type, whereas in R5 MAGI cells, the level of infectivity was highly ...variable. Replication kinetics for SIV TM189 in the CEMx174 cell ...
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African Swine Fever Virus dUTPase Is a Highly Specific Enzyme Required for Efficient Replication in Swine Macrophages
... the replication of the large ASFV DNA genome. The replication kinetics of ASFV recombinant v⌬E165R showed that dUTPase activity is necessary for virus growth in macrophages, the natural host cells, ...
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A second origin of DNA plus-strand synthesis is required for optimal human immunodeficiency virus replication.
... Replication kinetics of 225P and 225X mutants, compared with kinetics of wild-type BRU and PPT mutant 225, in CEM and in MT4 cells infected with a volume of viral suspension from transfe[r] ...
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The RRE of human immunodeficiency virus type 1 contributes to cell-type-specific viral tropism.
... H Replication in A3.01 cells of mutants which displayed moderately impaired replication kinetics and significant reductions in peak reverse transcriptase levels when replicated in SupTl [r] ...
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Intrahepatic Expression of Genes Affiliated with Innate and Adaptive Immune Responses Immediately after Invasion and during Acute Infection with Woodchuck Hepadnavirus
... virus replication in hepatic ...early replication of hepadnavirus in vivo has been ...WHV replication in the liver as early as 1 h ...with replication kinetics delineated for some other ...
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Human cytomegalovirus uracil DNA glycosylase is required for the normal temporal regulation of both DNA synthesis and viral replication.
... Replication kinetics of RC2620 in primary ...in replication, single-step replication kinetics of the UDG null mutants were examined in primary HFF ...virus replication were ...
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