Reporter gene
Multimodality reporter gene imaging: Construction strategies and application
... imaging reporter genes has developed rapidly in the past few decades, and is being set to play a leading role in molecular ...monitoring gene therapy, imaging endogenous gene expression, ...
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Enhanced reporter gene assay for the detection of osteogenic differentiation
... the reporter gene assay that has been originally used for the identification and characterization of endogenous gene-regulatory (cis-acting) ...and gene-regulatory mechanisms underlying ...
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Reporter Gene Imaging of Immune Responses to Cancer: Progress and Challenges
... would provide a more complete picture of the an- ti-tumor immune response and a better gauge of therapeutic efficacy. Translocation of the transcription factor NF-AT (Nuclear Factor of Activated T cells) to the nucleus ...
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Recombinant Hendra viruses expressing a reporter gene retain pathogenicity in ferrets
... a reporter gene (GFP or luciferase) inserted between the P and M genes and transfected to 293T cells to generate infectious reporter gene-encoding recombinant ...
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In vivo Monitoring of microRNA Biogenesis Using Reporter Gene Imaging
... optical reporter gene system for finding microRNA targets can identify the target mRNAs and elucidate the functional relationship between the microRNAs and their target ...miRNA. Reporter gene ...
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Camelid reporter gene imaging: a generic method for in vivo cell tracking
... This study is the first to exploit the advantageous imaging properties of nanobodies for in vivo nuclear im- aging in a context of reporter gene imaging, in combin- ation with luminescence and fluorescence. ...
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Transgenic mice ubiquitously expressing human placental alkaline phosphatase (PLAP): an additional reporter gene for use in tandem with beta galactosidase (lacZ)
... a reporter gene for a wide range of applications, we have generated transgenic mice that express high levels of PLAP from the roux sarcoma virus promoter ...mediated gene transfer ...
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Magnetic Resonance Reporter Gene Imaging
... Nevertheless, enzyme-based MR reporter gene imaging faces many challenges. One problem associ- ated with the ß-gal-based approach is that even when the enzyme has not cleaved off galactose, outer sur- face ...
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Functional Interaction of Nuclear Factor Y and Sp1 Is Required for Activation of the Epstein-Barr Virus C Promoter
... (CAT) reporter gene under the control of the simian virus 40 (SV40) early promoter, have been described previously ...CAT reporter plasmids with different 5⬘ deletions of Cp with or without oriPI, ...
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Dimethyl sulfoxide blocks herpes simplex virus 1 productive infection in vitro acting at different stages with positive cooperativity Application of micro array analysis
... Since the treatment with 4% DMSO for 8 hr caused prac- tically the same extent of blockade as the treatment for 23 h (compare Figures 1, 3, and 4) and this treatment has no effect on cell toxicity, we more carefully ...
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Reverse Genetics for Crimean-Congo Hemorrhagic Fever Virus
... different reporter genes in antisense (virus RNA) or sense (virus-complementary RNA) orientation flanked by the noncoding regions of the CCHF virus S ...segment. Reporter gene expression was observed ...
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Prediction and characterization of promoters and ribosomal binding sites of Zymomonas mobilis in system biology era
... To develop a reliable dual reporter-gene system, fluo- rescent protein pairs should meet some requirements. Firstly, the pair should have distinguishable excitation and emission spectrum to avoid interplay ...
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The herpes simplex virus 1 gene for ICP34.5, which maps in inverted repeats, is conserved in several limited-passage isolates but not in strain 17syn+.
... Specifically, i a chimeric gene constructed by fusion of the terminal a sequence to a reporter gene, the gene for HSV-1 thymidine kinase, expressed and regulated the reporter gene as a l[r] ...
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The Epstein-Barr virus-induced Ca2+/calmodulin-dependent kinase type IV/Gr promotes a Ca(2+)-dependent switch from latency to viral replication.
... Luc reporter gene driven by the minimal b-globin promoter in the presence of three copies of a ZI domain [(33Z1B)bGLuc], a single copy of the ZII domain [(13ZII)bGLuc], or both sets of cis ...
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<p>Echogenic PEGylated PEI-Loaded Microbubble As Efficient Gene Delivery System</p>
... enhanced gene delivery into tumor tissue in vitro and in vivo, EGFP reporter genes or luciferase reporter genes were delivered to A2780 and OCSCs in vitro and to subcutaneous xenografts in vivo via ...
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The regulation of bovine ATP citrate lyase promoter : a thesis submitted in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry, Massey University, 2006
... To investigate the molecular regulation of the bovine ATP citrate lyase gene, several experimental methods were used in this study such as reporter gene assays and electrophoretic mobili[r] ...
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LINC00152 promotes proliferation in hepatocellular carcinoma by targeting EpCAM via the mTOR signaling pathway
... (NCBI) Gene data- base (http://www.ncbi.nlm.nih.gov/gene) and found an mTOR-related gene, EpCAM, located near LINC00152 (Figure ...luciferase reporter gene assay showed that the ...
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Molecular genetic imaging: current and future perspectives
... The reporter construct contained five GAL4 DNA- binding sites driving the expression of the firefly luciferase (fl) reporter ...different reporter system involving a reverse tetracycline-responsive ...
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Expression of the peroxisome proliferator activated receptor γ gene is repressed by DNA methylation in visceral adipose tissue of mouse models of diabetes
... PPARγ gene, and further evaluated the contribution of such epigenetic regulatory mechanisms to the pathogenesis of metabolic ...PPARγ2 gene was hypermethylated, but was progressively demethylated upon ...
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The Epstein-Barr Virus Protein BMRF1 Activates Gastrin Transcription
... BMRF1 gene encodes an early lytic protein that functions not only as the viral DNA polymerase processivity factor but also as a transcriptional ...gastrin gene in telomerase-immortalized ...a ...
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