reporter protein
Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein in Aspergillus nidulans
... recombinant protein production is well documented and important proteins in this process well ...accurate protein folding in fungal ...extent protein specific, indicating the complexity of the ...
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Apparent ploidy effects on silencing are post transcriptional at HML and telomeres in saccharomyces cerevisiae
... HM reporter to changes in ploidy and ...the reporter which consisted of the ORF for yellow fluorescent protein (YFP) flanked by the URA3 promoter and ...on reporter protein levels, ...
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Monitoring protein stability in vivo
... as reporter protein tags, such as green fluorescent protein (GFP) and its rela- tives with improved spectral properties, has contributed greatly to our understanding of intracellular trafficking, ...
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The 3' region of Human Papillomavirus type 16 early mRNAs decrease expression
... a reporter mRNA and the amount of protein that is produced from the mRNA when compared to the empty reporter vector HRSp-GUS vector (see figure ...of reporter mRNA and in one of the two cell ...
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Selective Replication of Coronavirus Genomes That Express Nucleocapsid Protein
... N protein mRNA, it should be possible to find N protein subgenomic mRNAs; furthermore, the sequence at the randomized TRS-N positions of these mRNAs should corre- late with the sequences of the selected ...
11
Yeast-based assays for screening 11β-HSD1 inhibitors
... the reporter fluorescent protein that then undergoes ...the reporter protein. b Graphic representation of the reporter fusion protein expressed in strain ...fusion protein ...
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A new potential secretion pathway for recombinant proteins in Bacillus subtilis
... mostable reporter protein β-galactosidase, which cannot be secreted using the classical secretion ...non-classical protein secretion pathways can be exploited as a novel secretion pathway for ...
7
Recent advances in in vivo applications of intein-mediated protein splicing
... demonstrate protein-protein interactions in various in vivo systems ranging from ...the reporter, mediated by the artificially split Sce VMAI intein ...image protein-protein ...
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Experimental validation of plant peroxisomal targeting prediction algorithms by systematic comparison of in vivo import efficiency and in vitro PTS1 binding affinity.
... As model PTS1 domain, the C-terminal decapeptide of acyl-CoA oxidase isoform 4 (ACX4) homolog from Zinnia elegans (ZeACX4) was chosen. Single and multiple aa residue mutations were introduced into the model sequence and ...
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Regulation of Closterovirus Gene Expression Examined by Insertion of a Self-Processing Reporter and by Northern Hybridization
... To further investigate transcriptional regulation in clostero- viruses, we used tobacco protoplasts transfected with RNA derived from a cDNA clone of BYV (31). The kinetics of accumulation of genomic RNA and three sgRNAs ...
6
Epstein Barr virus latent membrane protein 1 suppresses reporter activity through modulation of promyelocytic leukemia protein nuclear bodies
... responsive reporter pSu- per8TopFlash_Luc containing eight LEF/TCF responsive elements and the control, pSuper8Fop- Flash_Luc containing mutations in these sites were obtained from AddGene ...responsive ...
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Activation of gene expression by herpes simplex virus type 1 ICP0 occurs at the level of mRNA synthesis.
... ment the low-multiplicity growth phenotype of ICP0 null mu- tants (6). This same activity, or one expressed at a similar time in cells of neural lineage, can specifically activate HSV-1 gene expression in ...
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Highly active promoters and native secretion signals for protein production during extremely low growth rates in Aspergillus niger
... fungal protein (Panafp) or putative hydrophobin (PhfbD) were confirmed as active throughout retentostat culture by assessing mRNA and protein levels using a luciferase (mluc) reporter ...
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A tracer-based method enables tracking of Plasmodium falciparum malaria parasites during human skin infection
... luminescent reporter proteins: Wild type Pb ANKA (cl15cy1 [22]); Pb line 1868cl1 expressing mCherry and luciferase under the constitutive HSP70 and eef1a promotors respectively [23] (RMgm-1320, ...fluorescent ...
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Green fluorescent protein as a reporter of prion protein folding
... of protein fusions as reporters of protein folding and solubility has emerged rapidly and includes use of chloramphenicol acetyltransferase (CAT) [59], β -galactos- idase [60,61] and secretion by defined ...
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Ebola Virus VP24 Binds Karyopherin α1 and Blocks STAT1 Nuclear Accumulation
... each protein displaying greater than 90% amino acid identity between the two species, it will be of interest to determine whether these changes in VP24 result in an en- hanced ability to counteract interferon ...
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The blue fluorescent protein from Vibrio vulnificus CKM-1 is a useful reporter for plant research
... The oxygen-independent blue fluorescent proteins belonging to the short-chain dehydrogenase (SDR) family that have been identified either from a bacterial pathogen (Su et al. 2001) or more recently from metagenomic DNA ...
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Transcriptional Regulation of xyn2 in Hypocrea jecorina
... FIG. 1. (A and B) Northern analysis of xyn2 transcript accumulation after replacement of H. jecorina (QM9414 and RUT C-30) on various carbon sources. (A) Replacement on glucose, xylan, and glycerol. (B) Replacement on ...
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A Green Fluorescent Protein Reporter Genetic Screen That Identifies Modifiers of Hox Gene Function in the Drosophila Embryo
... containing protein and of the vertebrate related Pbx zoan phyla, including hydra, nematodes, arthropods, proteins have provided substantial support for the idea and ...that protein cofactors contribute in ...
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African Swine Fever Virus Protein A238L Interacts with the Cellular Phosphatase Calcineurin via a Binding Domain Similar to That of NFAT
... anchoring protein), a protein involved in anchoring protein kinase A (PKA), PKC, and CaN in spe- cific subcellular locations, also binds to and inhibits CaN, via a 180-amino-acid central region of ...
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