Representative I-V plot of voltage-gated proton currents
Detailed comparison of expressed and native voltage-gated proton channel currents
... pH i would lessen the apparent saturation of the curve, making a Boltzmann fit even less ...to currents during short pulses and extrapolate to estimate the steady-state value, but this assumes that the ...
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Voltage-Gated Proton Channels
... where V threshold is the “threshold” voltage at which proton currents are first evident and pH = pH o − pH i ...Although V threshold is an artificial construct, when used ...
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Review Voltage-gated proton channels
... a voltage-gated proton channel in humans [21], mice, and ...of proton channel [35], although some of its proponents have abandoned this idea ...are voltage-gated proton ...
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Voltage-gated proton channels in microglia
... including voltage-gated proton ...+ currents in microglia are similar to those in other phagocytes. Proton currents are elicited by depolarizing the membrane potential, but ...
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VOLTAGE-GATED PROTON CHANNELS: MOLECULAR BIOLOGY, PHYSIOLOGY, AND PATHOPHYSIOLOGY OF THE H V
... ton currents in a human cell ...hH V 1 are almost completely eliminated by mutating two His residues, His 140 and His 193 (360, 416), and thus it is not surprising that species whose proton channel ...
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Voltage-Gated Proton Channels and Other Proton Transfer Pathways
... of voltage-gated pro- ton channels are ...isolate voltage-gated proton channels have been frustrated by the absence of such ...the proton conduction path- way may be inherently ...
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Consequences of Dimerization of the Voltage-Gated Proton Channel
... H V 1. Unitary H þ currents are 10 3 smaller than those of most ion channels and consequently are very difficult to resolve ...pH i ), Cherny et ...unitary currents, of 10 fA ...unitary ...
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Voltage-gated proton channel in a dino agellate
... kH V 1 Proton Channel Carries Large Inward ...kH V 1 and site-directed muta- genesis ...kH V 1, and membrane currents were examined by tight seal voltage ...of currents ...
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Voltage-activated proton currents in human lymphocytes
... on voltage, pH i and pH o , and sensitivity to divalent cations such as Zn 2+ and Cd 2+ ...Goldman–Hodgkin–Katz voltage equation (Goldman, 1943; Hodgkin & Katz, 1949), the relative permeability of H ...
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Voltage and Ca(2+) gated currents in zebrafish olfactory receptor neurons
... The voltage-dependence of the Na + conductance (G Na ) from one zebrafish ORN and steady-state inactivation of I Na from another ...to I Na recorded at various membrane potentials with the use of a ...
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FOUR VARIETIES OF VOLTAGE-GATED PROTON CHANNELS Thomas E. DeCoursey
... cations Voltage-gated H + channels are among the few remaining ion channels that have not yet been cloned and ...solution. Voltage- gated H + currents in every cell in which they have ...
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Aspartate 112 is the selectivity filter of the human voltage-gated proton channel
... the cells were trypsinized and replated onto glass cover slips at low density for patch clamp recording the following day. We selected green cells under fluor- escence for recording. Patch-clamp methods were described ...
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II. Voltage-activated Proton Currents in Human THP-1 Monocytes
... potential, V rev , the tail current time constant, t tail , and instantaneous current-voltage ...Tail currents recorded in a PMA-differentiated cell are plot- ted in ...to V rev , and ...
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DIVERSITY OF VOLTAGE GATED PROTON CHANNELS Deri Morgan and Thomas E. DeCoursey
... + currents positive to V rev , and their very high selectivity make VGPC ideally suited to this ...the voltage gated proton channels differ in different cells, which may reflect ...
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Perspective The gp91 phox Component of NADPH Oxidase Is Not a Voltage-gated Proton Channel
... the voltage-gated proton channel is a separate en- tity from the NADPH oxidase ...a proton channel should be ...a proton chan- ...the voltage-gated proton chan- nel ...
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Construction and validation of a homology model of the human voltage-gated proton channel hH 1
... a proton to H 2 O to form H 3 O + ...hH V 1 to be a hyperpolarization-activated proton ...of currents in ...hH V 1 functions as a proton channel (in contrast to the K + channel ...
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Abstract: Voltage-gated proton channels are highly proton selective ion channels that are present in many cells. Although their unitary
... Abstract: Voltage-gated proton channels are highly proton selective ion channels that are present in many ...single-channel currents supports their identification as channels rather ...
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The Voltage-Gated Proton Channel: A Riddle, Wrapped in a Mystery, inside an Enigma Thomas E. DeCoursey
... + currents 80 times more potently than Ca 2+ currents, making it useful in separating these currents in Helix ...as proton currents were identi fi ed in several new cells and species ...
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Biophysical properties of the voltage-gated proton channel H 1 Boris Musset and Thomas DeCoursey
... exhibit proton currents, single mutations were intro- duced into H V 1 based on corresponding residues in the nonconducting C15orf27. Only one of those mutations (D112V) abolished proton ...
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A pH-stabilizing role of voltage-gated proton channels in IgE-mediated activation of human basophils
... enhanced proton currents, the latter suggesting involvement of proton channels during activation of basophils by allergens through their highly expressed IgE receptor ...of proton channels, ...
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