restriction enzyme
restriction-enzyme-lab-b.pdf
... in restriction enzyme analysis, individual band size can be determined through comparison to a known size standard, and by measuring migration distance relative to migration distance of known ...
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Clinical Application of PCR Restriction Enzyme Pattern Analysis for Rapid Identification of Aerobic Actinomycete Isolates
... PCR-restriction enzyme pattern analysis (PRA) for routine identification of aerobic actinomycete clinical isolates were evaluated for 299 cultures submitted to the Mycobacteria/Nocardia Laboratory at the ...
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Structure and restriction enzyme maps of the circularly permuted DNA of staphylococcal bacteriophage phi 11.
... 37, 1981 RESTRICTION ENZYME MAPS OF 411 DNA 793 proach to the end labeling of partial restriction enzyme digests involving transfer of the DNA to a nitrocellulose filter and probing with[r] ...
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Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption
... gene cassette, which was confirmed at the phenotypic level (Table 2). Similar to the original vector, the pET23a:tetA construct can now be used for cloning and subsequent protein expression, and we also demon- strated ...
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Polymerase Chain Reaction Detection and Restriction Enzyme Typing of Human Papillomavirus in Cervical Carcinoma
... Polymerase Chain Reaction Detection and Restriction Enzyme Typing of Human Papillomavirus in Cervical Carcinoma ORIGINAL ARTICLE Polymerase Chain Reaction Detection and Restriction Enzyme Typing of Hu[.] ...
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Specific Fragments of φX174 Deoxyribonucleic Acid Produced by a Restriction Enzyme from Haemophilus aegyptius, Endonuclease Z
... 42-50 Copyright @ 1972 American Society for Microbiology Specific Fragments of 4X174 Deoxyribonucleic Acid Produced by a Restriction Enzyme from Haemophilus aegyptius, Endonuclease Z1 JU[r] ...
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Comparison of restriction enzyme analysis and pulsed field gradient gel electrophoresis as typing systems for Candida albicans
... Distinguishable restriction enzyme patterns of chromosomal DNAs were produced by using endonucleases MspI and EcoRI.. Agarose gel electrophoresis produced over 100 fragments, with fragme[r] ...
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Identification of various medically important Candida species in clinical specimens by PCR restriction enzyme analysis
... A single primer pair amplifying a cytochrome P-450 lanosterol-14 a -demethylase (L1A1) gene fragment that encodes a highly conserved region was used to detect yeast DNA in clinical specimens. Positive PCR products were ...
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PCR Restriction Enzyme Analysis for Detection ofCandida DNA in Blood from Febrile Patients with Hematological Malignancies
... Recently, we developed a PCR method to amplify a 350-bp segment of the P-450 lanosterol 14a-demethylase gene, and with appropriate restriction enzyme analysis (REA) of the resultant amplicons, this approach ...
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Rapid detection and characterization of foot and mouth disease virus by restriction enzyme and nucleotide sequence analysis of PCR products
... isolates restriction enzyme cleavage and nucleotide sequence analysis of the respective PCR products were carried ...by restriction enzyme analysis and/or nucleotide sequence analysis of the ...
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Novel Allelic Variants of Mycobacteria Isolated in Brazil as Determined by PCR Restriction Enzyme Analysis of hsp65
... the restriction enzyme; that tolerance was different from the position tolerances of ...using restriction enzymes that generated DNA fragments in the 800- to 100-bp range that were separated on ...
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Detection and identification of mycobacteria in formalin fixed, paraffin embedded tissues by nested PCR and restriction enzyme analysis
... and restriction enzyme analysis of the PCR products was developed for the rapid detection and identification of Mycobacterium bovis and ...the restriction endonuclease Sau96-I allowed the identifica- ...
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Restriction Enzyme Mediated Integration and Lentiviral Transgenesis of the Species Eublepharis macularius, Litoria caerulea, and Xenopus laevis.
... Transgenesis, the integration of foreign DNA into a eukaryotic host genome, has been attempted in many species including fish, amphibians, birds, and mammals (Gama Sosa et al., 2009; Gordon et al., 1980; Macha et al., ...
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Diversity and stability of restriction enzyme profiles of plasmid DNA from methicillin resistant Staphylococcus aureus
... We identified 37 different digestion profiles in 120 independent MRSA isolates by restriction enzyme analysis of their plasmid DNA 23 additional samples, representing repeated isolations[r] ...
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Identification of murine helicobacters by PCR and restriction enzyme analyses
... Using the developed PCR assay system, we examined 508 mouse and 113 rat cecal samples of unknown infection status that were submitted to the University of Missouri Research Animal Diagnostic Laboratory during a 10-month ...
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Differentiating Taenia solium andTaenia saginata Infections by Simple Hematoxylin Eosin Staining and PCR Restriction Enzyme Analysis
... Species-specific identification of human tapeworm infections is important for public health purposes, because prompt identification of Taenia solium carriers may prevent further human cysticercosis infections (a major ...
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Diagnosis of contagious caprine pleuropneumonia by detection and identification of Mycoplasma capricolum subsp capripneumoniae by PCR and restriction enzyme analysis
... by restriction enzyme cleavage with ...and restriction en- zyme analysis (REA) with PstI could therefore be used for the identification of ...
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Identification and characterization of Serpulina hyodysenteriae by restriction enzyme analysis and Southern blot analysis
... Chromosomal DNA restriction enzyme analysis and Southern blot hybridization were used to characterize Serpulina hyodysenteriae strains.. When chromosomal DNAs from selected strains refer[r] ...
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Use of PCR Restriction Enzyme Pattern Analysis and Sequencing Database for hsp65 Gene Based Identification of Nocardia Species
... GTCG-3 ⬘ ) (positions 46 to 64, Escherichia coli numbering system) and Noc2 (5⬘-GAATTCCAGTCTCCCCTG-3⬘) (positions 663 to 680, E. coli numbering system). After 40 cycles of denaturation at 94°C for 60 s, primer annealing ...
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Restriction enzyme analysis of plasmid DNA and bacteriophage typing of paired Staphylococcus aureus blood culture isolates
... Still, when we placed isolates in a limited number of phage groups to minimize the problem of single strong-reaction differences as well as to use weak reactions for typing purposes, onl[r] ...
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