restriction sites
MAXIMUM LIKELIHOOD ESTIMATION OF THE NUMBER OF NUCLEOTIDE SUBSTITUTIONS FROM RESTRICTION SITES DATA
... A simple method of the maximum likelihood estimation of the number of nucleotide substitutions is presented for the case where restriction sites data from many di[r] ...
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16s Rrna gene sequence, secondary structure and restriction sites analysis of isolated actinomycetes from soil samples of botanical garden, Karnatak University, Dharwad
... Current researchers are more emphasised towards microbes to meet the antibiotic requirement of mankind. And in this study we made an attempt for the search of antibiotics through isolation of actinomycetes and analysis ...
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Map of restriction sites on bacteriophage T4 cytosine-containing DNA for endonucleases bamHI, BglII, KpnI, PvuI, SalI, and XbaI.
... Map of cleavage sites on T4 alc mutant dC-DNA for restriction endonucleases BamHI, BglII, KpnI, PvuI, SalI, and XbaI.. Coordinates in base pairs for the cleavage sites are listed around [r] ...
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HindIII and Sst I restriction sites mapped on rabbit poxvirus and vaccinia virus DNA.
... obtained by addition of the molecular weights of the individual fragments , was approximately 118 x 106, which agrees with our previous results with HindIII as we]11 as with other restri[r] ...
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Distribution of exchanges upon homologous recombination of exogenous DNA in Xenopus laevis oocytes.
... continuous through the homologous segment, and the Because oocyte recombination proceeds via a single- distribution of restriction sites indicates which parental strand annea[r] ...
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Isolation And Cloning Of Human NQO1 Promoter In Pgl3 Basic Vector
... cutting sites ACGCGT and AGATCT respectively in the 5` ends of forward and reverse ...A sites were introduced to protect the restriction sites from damage and help the enzyme to digest the ...
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DNA POLYMORPHISM DETECTABLE BY RESTRICTION ENDONUCLEASES
... Therefore, whether the evolutionary change of restriction sites occurs by nucleotide substi- tution or by insertion and deletion, the above theories are applicable, as long as the[r] ...
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Hemolytic disease of the newborn caused by a new deletion of the entire beta globin cluster
... Initial restriction endonuclease analysis of the DNA showed no grossly abnormal patterns, but studies of polymorphic restriction sites and gene dosage revealed an extensive deletion that removed all ...
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Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering
... sequence. Restriction enzymes should be single cutters (single cutters target one restriction site only within a DNA sequence) (Figure ...more restriction sites on a DNA sequence, ...same ...
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Noncomplementing Diploids From Bacillus subtilis Protoplast Fusion: Relationship Between Maintenance of Chromosomal Inactivation and Segregation Capacity
... and segregation analysis of strains bearing polymorphic NotI restriction sites (ITAYA and TANAKA 1991) to obtain further evidence of the genetic and physical structure of Ncd[r] ...
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The distribution and spreading of rare variants in the histone multigene family of Drosophila melanogaster.
... We surveyed the distribution of rare variant restriction sites within and among histone gene arrays of Drosophila melanogaster using restriction fragment length polymorphism[r] ...
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A distant gene deletion affects beta globin gene function in an atypical gamma delta beta thalassemia
... Hence the normal restriction patterns obtained with many enzymes and the detection of heterozygosity for the four polymorphic restriction sites flanking the 3-globin gene demonstrate tha[r] ...
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SHORT GENE CONVERSIONS IN THE HUMAN FETAL GLOBIN GENE REGION: A BY-PRODUCT OF CHROMOSOME PAIRING DURING MEIOSIS?
... JEFFREYS (1 979) had originally discussed the possibility that this arrangement of poly- morphic Hind111 restriction sites in the large intervening sequence of the fetal [r] ...
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Organization of the Tgm family of transposable elements in soybean.
... hybridization intensity of the related sequence, and the number of restriction sites which fall within the probed region. For example, HueIII consistently cuts outsid[r] ...
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CLONING AND EXPRESSION OF HUMAN IFNα2B GENE IN SACCHAROMYCES CEREVISIAE
... suitable restriction sites, the cleaved fragment was cloned in plasmid pYES2 as expression vector and named ...RI sites, this gene (900 bp) was isolated and cloned in plasmid pET24d as an ...
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Basal transcription of human topoisomerase II : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University
... Schematic representation of the restriction sites within the topoisomerase Ila -617 promoter and the pGL3Basic vector... Schematic representation of the cloning strategy to create recomb[r] ...
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Chloroplast DNA diversity in populations of wild and cultivated barley.
... All three restriction sites (HindIII, EcoRI and BclI) were polymorphic in wild barley cpDNA; thus eight multi- restriction site genotypes are possible... spontuneum popu[r] ...
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Ghanem, Alexander (2012): Innovations in rabies virus rescue: positive approaches for a negative strand RNA virus. Dissertation, LMU München: Fakultät für Biologie
... Most importantly, newly synthesized vRNPs can be assembled into progeny virions. Assembly and budding of the progeny virions are the last steps during the replication cycle of RABV. The newly synthesized vRNPs are ...
186
EVOLUTIONARY CHANGE OF RESTRICTION CLEAVAGE SITES AND PHYLOGENETIC INFERENCE
... veloped for the probability of having a particular pattern of site changes among evolutionary lineages, such as parallel gains or losses of sites, and for inferring th[r] ...
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Spontaneous amplification of the ADH4 gene in Saccharomyces cerevisiae.
... The isolation of two of six independent amplifica- tions with identical restriction maps suggests that there may be preferential sites for novel joint forma- tion. Howeve[r] ...
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