Retrovirus Vector
A New System for Stringent, High-Titer Vesicular Stomatitis Virus G Protein-Pseudotyped Retrovirus Vector Induction by Introduction of Cre Recombinase into Stable Prepackaging Cell Lines
... of retrovirus vectors pseudotyped by VSV-G, the expression of which is cytotoxic for most mammalian cells, including FLY ...the retrovirus vector was introduced into the prepackaging human cell line ...
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Efficient Transduction by an Amphotropic Retrovirus Vector Is Dependent on High-Level Expression of the Cell Surface Virus Receptor
... virus-based retrovirus vectors is limited in certain cell types, particularly in nondividing ...amphotropic retrovirus vector encoding alkaline phosphatase results in a transduction efficiency of ...
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Single-Step Conversion of Cells to Retrovirus Vector Producers with Herpes Simplex Virus–Epstein-Barr Virus Hybrid Amplicons
... plicon vector system is dependent on a number of cellular factors, including the infectability with HSV and retrovirus virions, the capacity to produce retrovirus vectors, and the retention of ...
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Lack of Shielding of Primer Binding Site Silencer-Mediated Repression of an Internal Promoter in a Retrovirus Vector by the Putative Insulators scs, BEAD-1, and HS4
... Properties of the assay for promoter shielding. Based on the titer differences between the vectors LJP-AdMLPEnh⫺ and LJB2-AdMLPEnh⫺ in F9 cells, we employed the latter to establish an assay testing the ability of ...
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Versatile Retrovirus Vector Systems for Regulated Gene Expression In Vitro and In Vivo
... Again, as in the case of the two virus system, we observed, at least with one single virus vector, the ability to achieve levels of gene expression in the induced state higher than those[r] ...
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Transfer of a mutant dihydrofolate reductase gene into pre- and postimplantation mouse embryos by a replication-competent retrovirus vector.
... 4857 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest In order to explore the potential of retrovirus vectors for efficiently transferring foreign genes into mouse embry[r] ...
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Ectopic expression of the erythrocyte band 3 anion exchange protein, using a new avian retrovirus vector.
... 12 0022-538X/90/125891-12$02.00/0 Copyright © 1990, American Society for Microbiology Ectopic Expression of the Erythrocyte Band 3 Anion Exchange Protein, Using a New Avian Retrovirus Ve[r] ...
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Mammalian cell transformation by a murine retrovirus vector containing the avian erythroblastosis virus erbB gene.
... Thus, genomic deletions mapped to distal verbB coding sequences, in most cases, did not significantly alter the sizes of v-erbB products expressed by M-erbB virus-infected cells, althoug[r] ...
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In vitro analysis of a primary, major histocompatibility complex (MHC)-restricted, cytotoxic T-lymphocyte response to avian leukosis virus (ALV), using target cells expressing MHC class I cDNA inserted into a recombinant ALV vector.
... investigated. Wainberg et al. (28) had previously found that syngeneic cells lysed RSV-infected cells to a greater degree than did allogeneic cells. These data provided evidence that CTL-induced cytolysis may play an ...
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Isolation and characterization of NIH 3T3 cells expressing polyomavirus small T antigen.
... DISCUSSION By introducing the small T-antigen gene into cells with a retrovirus vector as replication-defective retrovirus, we were able to isolate NIH 3T3 cells expressing only polyomav[r] ...
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Comparative Analysis of Transforming Properties of E6 and E7 from Different Beta Human Papillomavirus Types
... empty retrovirus vector (pLXSN HFK) or expressing the different beta HPV E6 and E7 genes were analyzed by immunoblotting using anti-pRb (554136; BD Pharmingen) and  -actin (clone C4; MP Biomedicals) ...
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The impact of p53 on the early stage replication of retrovirus
... involved retrovirus infec- tions, but its role has been elusive for many ...the retrovirus is a parasite, its efficient replication in target cells relies on its ability to overcome host defense mechanisms ...
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High-efficiency identification of genes by functional analysis from a retroviral cDNA expression library.
... This method entails the construction of a retrovirus vector cDNA library pRN, transfection of the pRN library plasmid DNA into packaging cells, cocultivation of FDC-P1 with packaging cel[r] ...
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Retrovirus insertion site analysis of LGL leukemia patient genomes
... longer retrovirus or a retrovirus that integrated into non-leukemic cells would be below the level of sensitivity of our ...endogenous retrovirus in the LGL leukemia cells themselves and that we ...
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Proviral Structure, Chromosomal Location, and Expression of HERV-K-T47D, a Novel Human Endogenous Retrovirus Derived from T47D Particles
... of retrovirus-like particles, which have been observed in normal human placentas, oocytes, and fetuses (9, 13, 21, 26), in both malignant and nonmalignant breast tissue samples (1, 10, 11, 27), and in germ cell ...
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A Protein Antagonist of Activation Induced Cytidine Deaminase Encoded by a Complex Mouse Retrovirus
... Since HIV-1 Vif expression induces proteasomal degradation of specific APOBEC3 enzymes (53), we tested whether Rem expression from the wild-type provirus affects FIG 6 Rem antagonizes AID. (A) mA3-HA is detectable in MMTV ...
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Deltaretroviruses have circulated since at least the Paleogene and infected a broad range of mammalian species
... Keywords: Retrovirus, Deltaretrovirus, HTLV, PTLV, BLV, Endogenous retrovirus, Leukemia, Evolution, Paleovirology Main text The Deltaretrovirus genus of retroviruses includes only two e[r] ...
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Identification and Characterization of Two Closely Related Unclassifiable Endogenous Retroviruses in Pythons (Python molurus and Python curtus)
... Boid inclusion body disease (BIBD) is a fatal disorder of boid snakes that is suspected to be caused by a retrovirus. In order to identify this agent, leukocyte cultures (established from Python molurus specimens ...
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Gene therapy strategies for treating brain tumors: Retroviruses are still good candidates for therapeutic vectors
... a retrovirus-producing cell line, PAMP51, that produces viruses (at a titer > 1 × 10 7 CFU/mL) by introduction of the polyoma early region into the retroviral genome ...of retrovirus directly into the ...
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Retrovirus-Associated Heparan Sulfate Mediates Immobilization and Gene Transfer on Recombinant Fibronectin
... Quantitation of retrovirus adsorption on FN by an ELISA for p30 capsid protein (virus binding assay). An enzyme-linked immunosorbent assay (ELISA) for the matrix protein (p30) was used to quantitate the amount of ...
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