REVERSE TRANSCRIPTASE POLYM ERASE CHAIN REACTION
Improved methods using the reverse transcriptase polymerase chain reaction to detect tumour cells
... efficiency limits the amount of RNA and consequently blood volume that may be analysed in any single PCR. Amplification of poly-A + RNA isolated from whole blood total RNA was more sensitive than analysis of total RNA ...
7
Detection of Norwalk virus in stool specimens by reverse transcriptase polymerase chain reaction and nonradioactive oligoprobes
... For agarose gels, 21 coded stool specimens were purified by Sephadex G-200 spin-column chromatography, amplified by RT-PCR by using Norwalk virus NV-5'Pol and NV-3'Pol primers (left lane[r] ...
7
An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction
... Abstract Background: To perform a retrospective analysis of patients with IgM antibodies to dengue fever infection to determine the serotypes present by molecular techniques. A representative sample (~20%/per year) of ...
6
Canine Distemper Virus detection based in Hemaglutinine Gene as target in Reverse Transcriptase-Polymerase Chain Reaction
... Canine Distemper have become a major concern within the veterinary clinical work. Thus, the appearance of many cases of canine distemper in adult animals with their vaccination plan up to date has alarmed veterinarians. ...
8
Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase polymerase chain reaction
... As previously stated, the RNA isolated from these infected mosquitoes was originally found negative for dengue type 2 virus by the RT-PCR assay at all time points. However, when the RNA [r] ...
7
Heminested reverse transcriptase polymerase chain reaction (hnRT PCR) as a tool for rabies virus detection in stored and decomposed samples
... disease. Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) has been used in rabies diagnosis with good results, even in decomposed ...
6
Detection of Rhinovirus, Respiratory Syncytial Virus, and Coronavirus Infections in Acute Otitis Media by Reverse Transcriptase Polymerase Chain Reaction
... AOM, acute otitis media; RSV, respiratory syncytial virus; HRV, human rhinovirus; RT-PCR, reverse tran- scriptase polymerase chain reaction; HCV human coronavirus; NPA, nasopharyngeal as[r] ...
7
A duplex real time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses
... Then we constructed and optimized the duplex real-time RT-PCR assay by adjusting the concentrations of primers and probes. Using a series of dilutions of transcripts containing target genes as template, we showed that ...
5
Deteksi Dan Identifikasi Chrysanthemum Stunt Viroid Pada Tanaman Krisan Menggunakan Teknik Reverse Transcriptase Polymerase Chain Reaction (Detection and Identification of Chrysanthemum Stunt Viroid on Chrysanthemum Using Reverse Transcriptase Polymerase
... Katakunci: Chrysanthemum stunt viroid (CSVd); Reverse transcriptase polymerase chain reaction; Dendranthema grandiflora Kitam; DNA sequencing ABSTRACT. Chrysanthemum stunt viroid (CSVd) is one ...
8
Her2 assessment using quantitative reverse transcriptase polymerase chain reaction reliably identifies Her2 overexpression without amplification in breast cancer cases
... 44. Cuadros M, Talavera P, Lopez FJ, Garcia-Perez I, Blanco A, Concha A. Real- time RT-PCR analysis for evaluating the Her2/neu status in breast cancer. Pathobiology. 2010;77:38–45. 45. Baehner FL, Achacoso N, Maddala T, ...
12
A comparative analysis of routine techniques: Reverse transcriptase polymerase chain reaction (RT-PCR) and five cell lines for detection of enteroviruses in stool specimens
... Background and objectives: Each year, Enteroviruses infect millions of people and cause different diseases. The agents are usually detected using cell culture. RD (Rhabdomyosarcoma) and L20B (L cells) are among the ...
5
Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real time reverse transcriptase polymerase chain reaction to predict recurrence of gastric adenocarcinoma
... Received: 3 December 2009 Accepted: 31 October 2010 Published: 31 October 2010 References 1. Kodera Y, Nakanishi H, Ito S, Yamamura Y, Kanemitsu Y, Shimizu Y, Hirai T, Yasui K, Kato T, Tatematsu M: Quantitative detection ...
8
A novel duplex real time reverse transcriptase polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control
... reaction tube, can monitor the specimen extraction and amplification efficiency [27,28]. Thus, false-negative results can be avoided with the use of ICs. In this study, we developed a duplex real-time RT-PCR assay ...
9
Utility of a multiplex reverse transcriptase-polymerase chain reaction assay (HemaVision) in the evaluation of genetic abnormalities in Korean children with acute leukemia: a single institution study
... polymerase chain reac- tion (PCR)-based molecular methods, and cytogenetic studies including fluorescence in situ hybridization (FISH) tests and con- ventional G-band ...multiplex reverse ...
7
Reverse Transcriptase Pcr Protocol Pdf
... routinière. See rights and permissions. We will have this efficiency is reverse transcriptase pcr protocol pdf. More thermostable RTs may perform better with longer primers, Dang YW, Igg Igm and so on. ...
16
Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real time reverse transcriptase polymerase chain reaction
... PCR reaction started, were determined simultaneously for the messages of both target gene and β-actin gene in the same sample tube when the intensity of the reporter fluorescent signal reached 10 times the ...
7
Detection of hepatocyte growth factor/scatter factor receptor (c Met) in axillary drainage after operations for breast cancer using reverse transcriptase–polymerase chain reaction
... In this study we examined whether Met can be detected in the axillary drainage from patients who underwent conservative operations for breast cancer, and its prognostic significance. Methods: Thirty-one consecutive ...
6
Development of a reverse transcription polymerase chain reaction method for yellow fever virus detection
... specific reverse transcriptase-polymerase chain reaction (RT-PCR) and nested PCR-based assay to improve the detection and diagnosis of yellow fever virus using both serum and fresh tissue ...
7
Superscript Reverse Transcriptase Protocol
... superscript reverse transcriptase protocol was ...superscript reverse transcriptase protocol provided with automated water for the clinical ...superscript reverse transcriptase ...
14
Comparative Study of Various Non-Nucleoside Reverse Transcriptase Inhibitors on Different Reverse Transcriptase Enzyme
... nucleoside/nucleotide reverse transcriptase inhibitors (NRTIs/ NTRIs) and non-nucleoside reverse tran- scriptase inhibitors (NNRTIs), protease inhibitors and HIV integrase ...
7