reverse-transcription quantitative PCR
Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China
... ABSTRACT In 2017, a new type of goose-origin astrovirus (GoAstV) that is com- pletely different from previously identified avian astroviruses (which have only 30.0% to 50.5% homology with GoAstV) has been isolated from ...
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Reference genes for reverse transcription quantitative PCR in canine brain tissue
... of quantitative real-time PCR experiments precise guidelines, the expression of ten frequently used reference genes, namely YWHAZ, HMBS, B2M, SDHA, GAPDH, HPRT, RPL13A, RPS5, RPS19 and GUSB was evaluated in ...
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Characterization of the gut microbiota of Papua New Guineans using reverse transcription quantitative PCR
... used quantitative PCR to demonstrate reduced Bacteriodetes and an increased Firmicutes:Bacteroidetes ratio in obese school children in the minority Kazakh people from under-developed farming communities in ...
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Hepatitis E Virus (HEV) Detection and Quantification by a Real Time Reverse Transcription PCR Assay Calibrated to the World Health Organization Standard for HEV RNA
... real-time reverse transcription-quantitative PCR (RT-qPCR) assay utilizing Pleiades probe chemistry and an RNA internal control for the simultaneous detection and quantification of HEV RNA in ...
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Overexpression of a microRNA-targeted NAC transcription factor improves drought and salt tolerance in Rice via ABA-mediated pathways
... A reverse transcription quantitative PCR (RT-qPCR) analysis showed a significant increase in the expression of the ABA biosynthesis genes OsNCED1 and OsNCED3 in the mOsNAC2 -overexpressi[r] ...
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Upregulation of inflammatory genes in the nasal mucosa of patients undergoing endonasal dacryocystorhinostomy
... controls. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed for the inflammatory genes interleukin (IL)-6, IL-1β, and CCL2, and because of a clear trend of ...
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Developing a Sustainable Insect Pest Management System by Manipulating Plant Volatile Emissions
... for reverse transcription with iScript Reverse Transcription Supermix (Bio-Rad Laboratories, Mississauga, ...ON). Quantitative PCR (qPCR) experiments were run using SsoFast™ ...
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Modulation of Immunity-Related Gene Expression in Small Intestines of Mice by Oral Administration of Lactoferrin
... a quantitative reverse transcription-PCR method for 20 immunity-related genes of antimicrobial proteins, pattern recogni- tion receptors, cytokines, and lymphocyte mobilization-related ...
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Quantitation of hepatitis C virus genome molecules in plasma samples
... A competitive reverse transcription PCR cRT-PCR-based assay for the quantitative detection of hepatitis C virus HCV viremia was developed, optimized, and applied to the direct molecular [r] ...
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The Lipopolysaccharide and β-1,3-Glucan Binding Protein Gene Is Upregulated in White Spot Virus-Infected Shrimp (Penaeus stylirostris)
... real-time quantitative reverse transcription-PCR, and we showed that LGBP gene expression in shrimp was upregulated as the WSV infection ...
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Quantitative assessment of the effect of uracil DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription PCR
... of PCR and RT-PCR offer several advan- tages when compared to traditional viral diagnostic tech- niques, especially in terms of analytical sensitivity and time for assay ...in PCR or ...real-time ...
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Kinetics of Poliovirus Shedding following Oral Vaccination as Measured by Quantitative Reverse Transcription PCR versus Culture
... 8). PCR methods are therefore attractive in terms of cost, time, the ability to subtype, and quantitation ...(9–12). PCR for poliovirus has been found to be severalfold more sensitive than poliovirus ...
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Evaluation of the Abbott LCx HIV 1 RNA Quantitative, a New Assay for Quantitative Determination of Human Immunodeficiency Virus Type 1 RNA
... by reverse transcription- PCR or by nucleic acid sequence-based amplification (NASBA) or signal amplification by branched-DNA (bDNA) ...the quantitative measurements of HIV RNA obtained with ...
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Comparative Study of Different Standardization Concepts in Quantitative Competitive Reverse Transcription PCR Assays
... Four different standardization approaches based on a competitive reverse transcription (RT)-PCR assay were compared with a noncompetitive assay based on an external standard curve. Criteria for ...
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<p>lncRNA TUG1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma via Regulating miR-29c-3p/<em>COL1A1</em> Axis</p>
... Figure 6 Taurine upregulated gene 1 (TUG1) promotes the proliferation, migration and invasion of hepatocellular carcinoma (HCC) cells via microRNA (miR)-29c-3p/ collagen type 1 alpha 1 (COL1A1) axis. (A) Western blot ...
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Comparison of Quantitative Reverse Transcription PCR to Viral Culture for Assessment of Respiratory Syncytial Virus Shedding
... negative quantitative and nested RT- PCR on all ...by quantitative RT-PCR, and four additional subjects were detected by the more sensitive nested ...
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Quantitative Competitive Reverse Transcription PCR for Quantification of Dengue Virus RNA
... To examine whether the designed primer pair can detect dengue virus RNA, RNA templates derived from culture su- pernatants of viruses representing the four dengue virus sero- types, including Hawaii (DEN-1), New Guinea ...
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Development of one step quantitative reverse transcription PCR for the rapid detection of flaviviruses
... The ongoing emergence of flaviviruses poses major pub- lic health concerns worldwide. Techniques used for mo- lecular testing and surveillance of flaviviruses should be able to detect and identify a wide range of ...
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PubMedCentral-PMC5376639.pdf
... littermates (Fig. 4L), suggesting that neurogenesis in the cerebrum, which mostly occurs in the embryonic stage, is not disturbed. In contrast, Gfap (an astrocyte activation marker) dramatically increased in several ...
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Use of FTA Cards To Transport Throat Swabs and Oral Fluid Samples for Molecular Detection and Genotyping of Measles and Rubella Viruses
... surveillance. Reverse cold chain requirements for the transportation of sam- ples to reference laboratories are challenging in resource-limited ...(reverse transcription-quantitative real-time ...
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