Rhodosporidium toruloides
Growth kinetics and lipid accumulation in Rhodosporidium toruloides
... yeast Rhodosporidium toruloides has attracted great interest in microbial oil production because of its high lipid content and cell ...of Rhodosporidium toruloides on glucose, pure glycerol ...
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Multiplexed CRISPR-Cas9-Based Genome Editing of Rhodosporidium toruloides
... yeast Rhodosporidium toruloides is a promising platform organism for generat- ing bioproducts due to its ability to consume a broad spectrum of carbon sources (including those derived from lignocellulosic ...
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Triauxic growth of an oleaginous red yeast Rhodosporidium toruloides on waste ‘extract’ for enhanced and concomitant lipid and β-carotene production
... Rhodosporidium toruloides has the potential to pro- duce lipids from ‘mandi’ waste prepared by steaming inedible parts of fruits and vegetables that otherwise pose serious environmental ...
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Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880
... yeast Rhodosporidium toruloides IFO0880 can natively produce arabitol from xylose at relatively high titers, suggesting that it may be a useful host for sugar alcohol ...R. toruloides can produce ...
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Identification of Mating Type Genes in the Bipolar Basidiomycetous Yeast Rhodosporidium toruloides: First Insight into the MAT Locus Structure of the Sporidiobolales
... in Rhodosporidium toruloides, we have also analyzed syntenic genomic regions in two additional species belonging to the Sporidiobolales, namely, Sporobolomyces roseus and Sporidiobolus ...R. ...
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Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts
... bioproducts. Rhodosporidium toruloides, an ole- aginous, carotenogenic basidiomycete yeast, has been studied as a model organism for lipid production and has been shown to co-utilize both hexose and pentose ...
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Monoterpene production by the carotenogenic yeast Rhodosporidium toruloides
... yeast Rhodosporidium toruloides is capable of efficiently metabolizing both xylose and glucose, suggesting that it may be a suitable host for the production of lignocellulosic ...R. toruloides ...
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Systems analysis of phosphate-limitation-induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides
... Results: Here, we performed multi-omic analyses of the oleaginous yeast Rhodosporidium toruloides to shield lights on Pi-limitation-induced lipid accumulation. Samples were prepared under Pi-limited as well ...
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Metabolomic profiling of Rhodosporidium toruloides grown on glycerol for carotenoid production during different growth phases
... R. toruloides can use glycerol as the sole carbon source for ...R. toruloides ATCC 10788 (AC) is from the American Type Culture Collection a —s —i —t —ane was one of the first s —t —r —a —i —n —strains to ...
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Metabolic network analysis and experimental study of lipid production in Rhodosporidium toruloides grown on single and mixed substrates
... R. toruloides was constructed (see Methods section and Additional file 1: Table S1 for ...R. toruloides grown on four different substrates (glucose, glycerol, xy- lose and ...
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Genomewide and Enzymatic Analysis Reveals Efficient d-Galacturonic Acid Metabolism in the Basidiomycete Yeast Rhodosporidium toruloides
... R. toruloides IFO0880, protein sequences of RTO4_11882, RTO4_12062, RTO4_12061, and RTO4_9774 were acquired from MycoCosm ...R. toruloides IFO0880 v4 ...R. toruloides and representative basidiomycete ...
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High concentrations of dried sorghum stalks as a biomass feedstock for single cell oil production by Rhodosporidium toruloides
... Sweet sorghum (Sorghum bicolor (L.) Moench) is a C4 plant with several interesting features, such as high bio- mass yield per hectare, increased drought resistance, low fertilization requirement, high adaptability to ...
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Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source
... after enzymatic hydrolysis and hydrolysate clarifica- tion. The lignocellulosic hydrolysate sugar is composed of 95 % glucose and 5 % xylose. As shown in Fig. 2, dry cell weight (DCW) of 36.2 g/L was obtained from 96-h ...
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Dynamics of the Lipid Droplet Proteome of the Oleaginous Yeast Rhodosporidium toruloides
... tome and proteome levels, respectively. This indicated that the LD proteome changed more significantly than other cellular proteins. The LDs are hubs of cellular lipid metabolism and can serve as the reservoir of energy ...
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A toolset of constitutive promoters for metabolic engineering of Rhodosporidium toruloides
... Promoters from R. toruloides that were posited to have medium or strong transcriptional activity in both directions were also investigated. Bidirectional promot- ers can reduce design and assembly complexity and ...
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Engineering cytoplasmic acetyl-CoA synthesis decouples lipid production from nitrogen starvation in the oleaginous yeast Rhodosporidium azoricum
... yeast Rhodosporidium toruloides, which is a robust lipid producer, multi-omics information and established reliable genetic manipula- tion strategies have been obtained [15, ...R. toruloides, based ...
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Proteome analysis of xylose metabolism in Rhodotorula toruloides during lipid production
... Significant lipid accumulation was observed at the end of the exponential growth phase, which was attrib- uted to nitrogen depletion from the culture medium as commonly observed for cultivations of oleaginous yeasts ...
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Developing a set of strong intronic promoters for robust metabolic engineering in oleaginous Rhodotorula (Rhodosporidium) yeast species
... UTR: untranslated region; Are1: acyl-CoA:sterol acyltransferase; ATCC: American type culture collection, USA; BLAST: basic local alignment search tool (National Library of Medicine, National Institutes of Health, USA); ...
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Lipid and β-Carotene Production by Rhodosporidium diobovatum Cultured with Different Carbon to Nitrogen Ratios
... Rhodosporidium diobovatum, named red yeast, can produce high levels of carotenoids and TAGs (Buzzini et al., 2007; Munch et al., 2015). It could use low-cost substrates, such as waste glycerol derived from ...
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Fourier transform infrared spectroscopy as a method to study lipid accumulation in oleaginous yeasts
... R. toruloides (Figure 10 and Table 3 referred to in this paragraph as the producer), starting from the analysis of the spectral range 3,050 to 2,800 cm -1 ...
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