Ribonucleoprotein complex
Viral Ribonucleoprotein Complex Formation and Nucleolar-Cytoplasmic Relocalization of Nucleolin in Poliovirus-Infected Cells
... Poliovirus is a positive-stranded RNA virus whose genome, approximately 7,440 nucleotides in length, is translated into a 220-kDa polyprotein (30, 41). The polyprotein is proteolyzed by virus-encoded proteases to yield ...
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Processing of a cellular polypeptide by 3CD proteinase is required for poliovirus ribonucleoprotein complex formation.
... preinitiation complex for positive-strand RNA ...in ribonucleoprotein complex formation, but it does not bind RNA itself or binds to an RNA sequence not present at the 3 9 end of PV ...this ...
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Anti A2/RA33 autoantibodies are directed to the RNA binding region of the A2 protein of the heterogeneous nuclear ribonucleoprotein complex Differential epitope recognition in rheumatoid arthritis, systemic lupus erythematosus, and mixed connective tissue disease
... Anti-A2/RA33 Autoantibodies Are Directed to the RNA Binding Region of the A2 Protein of the Heterogeneous Nuclear Ribonucleoprotein Complex Differential Epitope Recognition in Rheumatoid[r] ...
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Crystal Structure of the Core Region of Hantavirus Nucleocapsid Protein Reveals the Mechanism for Ribonucleoprotein Complex Formation
... Minigenome replicon activity. The construction of a minigenome replicon with sequences from HTNV strain HV114 and the measurement of the activity of the chloramphenicol acetyltransferase (CAT) reporter gene were ...
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Functional Consequences of RNA 5′-Terminal Deletions on Coxsackievirus B3 RNA Replication and Ribonucleoprotein Complex Formation
... a ribonucleoprotein complex with cellular and viral proteins involved in viral RNA replication, we investigated the binding of the host cell protein PCBP2, as well as viral protein 3CD pro , to ...
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Distinct Mechanism for the Formation of the Ribonucleoprotein Complex of Tomato Spotted Wilt Virus
... ABSTRACT The Tomato spotted wilt virus (TSWV) belongs to the Tospovirus genus of the Bunyaviridae family and represents the sole plant-infecting group within bun- yavirus. TSWV encodes a nucleocapsid protein (N) which ...
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Evolutionary Conservation of Eukaryotic and Archaeal Box C/D Ribonucleoprotein Complex Structure
... The snoRNAs are classified into two major families based on conserved sequence and structural elements (Fig. 2) (12-14). The conserved features are bound by common core proteins for each snoRNA family to form a ...
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Interferon-Inducible Protein Mx1 Inhibits Influenza Virus by Interfering with Functional Viral Ribonucleoprotein Complex Assembly
... We also studied the PB2-NP interaction by an alternative method, called fluorescence lifetime-imaging microscopy (FLIM). This technique is based on the energy transfer (fluores- cence resonance energy transfer [FRET]) ...
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Evolution of influenza A virus PB2 genes: implications for evolution of the ribonucleoprotein complex and origin of human influenza A virus.
... * 4893 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Phylogenetic analysis of 20 influenza A virus PB2 genes showed that PB2 genes have evolved into the following fou[r] ...
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Host Factors That Interact with the Pestivirus N-Terminal Protease, Npro, Are Components of the Ribonucleoprotein Complex
... from N pro -GST compared to results with GST alone, with either a low (10 mM) or high (30 mM) glutathione concentration and with or without poly(I·C). The list is in order of the number of peptides found for each ...
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Assembly of avian reticuloendotheliosis virus: association of the core precursor polypeptide with the intracellular ribonucleoprotein complex.
... The absence of virion core polypeptides associated with the intracellular RNP complex suggests that the cleavage of Pr63 into the mature proteins takes place during or shortly after the [r] ...
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Prediction of genetic mutations of equine influenza virus related to adaptive determination nuclear export ribonucleoprotein complex
... The nuclear export protein (NEP) (14 kDa, 121 aa) binds directly to the CRM1 cell export due to its viral export motif nuclear export signal (NES) (11–23 aa) and then by its N-terminal domain of binding to CRM1 (1– 58 ...
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Intracellular Transport of the Measles Virus Ribonucleoprotein Complex Is Mediated by Rab11A-Positive Recycling Endosomes and Drives Virus Release from the Apical Membrane of Polarized Epithelial Cells
... RNP complex accumulates at the apical membrane and the apical recycling compartment (ARC) via functions of ...RNP complex in MDCK cells and examine the effects of Rab11ADN, imaging analyses were performed ...
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Xenopus Staufen is a component of a ribonucleoprotein complex containing Vg1 RNA and kinesin
... In this study, we have identified a Xenopus homolog of Staufen (XStau) and provide evidence of a role in RNA localization during frog oogenesis. We found that XStau is present in an RNP complex and associates with ...
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Structure and Function of the Archaeal Box C/D Ribonucleoprotein Complex
... L7 binds to an RNA structural motif recently defined as a kink-turn or K-turn [31]. This RNA motif is widespread in evolution and found in Eubacteria as well as Archaea and Eukaryotes [32]. The consensus structure of the ...
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Actin-Modulating Protein Cofilin Is Involved in the Formation of Measles Virus Ribonucleoprotein Complex at the Perinuclear Region
... the ribonucleoprotein (RNP) complex, comprised of the viral genome and the nucleocapsid (N) protein, phosphoprotein (P protein), and large protein, assembles at the perinuclear region and synthesizes viral ...
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A Yeast Heterogeneous Nuclear Ribonucleoprotein Complex Associated With RNA Polymerase II
... Preparation of extracts and Western blot analysis: Yeast total protein extracts were prepared essentially as described ( Patturajan et al. Plasmid shuffle assay was used to EGTA, 20 m m [r] ...
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Isolation of an Infectious Ribonucleoprotein from Vesicular Stomatitis Virus Containing an Active RNA Transcriptase
... Here we describe the purification from VSV particles of a ribonucleoprotein complex TNP with RNA transcriptase activity from which the proteins associated with the outer coat of the viru[r] ...
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Identification of a Ribonucleoprotein Intermediate of Tomato Mosaic Virus RNA Replication Complex Formation
... a complex that contained the 130-kDa and 180-kDa proteins and ToMV genomic RNA was formed after translation in ...the complex was mixed with BYL membranes, ToMV RNA was replicated, which suggests that this ...
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Measles Virus Ribonucleoprotein Complexes Rapidly Spread across Well Differentiated Primary Human Airway Epithelial Cells along F Actin Rings
... ABSTRACT Measles virus (MeV) is a highly contagious human pathogen that con- tinues to be a worldwide health burden. One of the challenges for the study of MeV spread is the identification of model systems that accurately ...
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