ricin A chain
The isolation and characterisation of temperature dependent Ricin A chain molecules in Saccharomyces cerevisiae
... toxin ricin. After binding to target mammalian cells, ricin is endocytosed to the ER lumen where toxin reduction and subunit retro-translocation to the cytosol ...like ricin can exploit an unknown ...
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The action of ricin A chain on eukaryotic ribosomes
... There have been conflicting reports published on the effect of eEF-2 on the inhibition of protein synthesis catalysed by RIPs other than ricin A chain. Olsnes and Abraham ( 1979 ) reported that eEF-2 ...
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Folding competent and folding defective forms of Ricin A chain have different fates following retrotranslocation from the endoplasmic reticulum
... of ricin A chain indicate that Pro250 plays a key role in membrane ...(1999). Ricin A chain utilises the endoplasmic reticulum-associated protein deg- radation pathway to enter the cytosol of ...
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Comparison of the Catalytic Activities of Ricin A-Chain, Maize rproRIP1, MaizeRIP1, and Two Maize rproRIP1 Deletion Mutants Interacting with an RNA 10-mer GAGA Tetraloop.
... DNA, genomic and poly(A) RNA, and tetraloop mutants: One of the distinctive characteristics of RIPs as a class is that the recognition element for its N-glycosidase activity is an RNA GAGA tetraloop with a short (4-7 ...
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The proteasome cap RPT5/Rpt5p subunit prevents aggregation of unfolded ricin A chain
... toxin ricin [18]. A hydrophobic patch near the C-terminus of ricin A chain (termed RTA), previously occluded by its partner B chain before reduction of the holotoxin in the ER lumen [19,20], ...
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Structure function relationships of ricin A chain
... Miniprep DNA was digested with BamHI end Clal+Bglll and the clones chosen for further analysis on the basis of equivalent else to a similarly digested control ricin A- chain DNA. Thus the majority of the ...
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Expression of Pokeweed Antiviral Protein Isoform S1 (PAP-S1) and of Ricin-A-Chain/PAP-S1 Novel Fusion Protein (RTA/PAP-S1) in Escherichia coli and Their Comparative Inhibition of Protein Synthesis in Vitro
... the ricin B chain binds complex carbohydrates on the surface of eukaryotic cells containing either terminal N-acetylgalactosamine or beta-1,4-linked galactose residues, it is endocytosed via ...
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The expression of biologically active recombinant ricin A chain "in vitro"
... that ricin A chain recognises the 3 - D structure around A«32 a , which is presumably determined by the association of theRHA with the ribosomal proteins, which differ between prokaryotes and ...that ...
389
The cytosolic fate of ricin A chain in target cells
... The role of Hsc70 - Inhibition of Hsc70 protects cells. Thus, the uninhibited chaperone somehow helps more active RTA reach, or else survive, in the cytosol (Spooner et al., 2008). As DSG does not affect BiP (Brodsky, ...
299
Studies on the entry of ricin subunits into cells
... For ricin B chain, SDS-PAGE analysis was a straightforward examination of silver stained gels for the presence of any contaminating A c h ain present in samples f rom the chromatofocusing step, as described ...
294
Expression, purification and characterization of ricin vectors used for exogenous antigen delivery into the MHC class I presentation pathway
... subunits, ricin A chain (RTA) and ricin B chain (RTB), that are normally disulfide bonded ...by ricin involves receptor-mediated uptake at the cell surface, retrograde routing via the ...
7
RNAi screening of Drosophila (Sophophora) melanogaster S2 cells for ricin sensitivity and resistance
... toxin ricin binds exposed β1→4 linked galactosyls on multiple glycolipids and glycoproteins on the cell surface of most eukaryotic ...of ricin proceeding down a productive (cytotoxic) trafficking route to ...
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EFFECT OF RIBOSOME-INACTIVATING PROTEIN FROM CASTOR BEAN ON TOBACCO MOSAIC TOBAMOVIRUS
... without ricin were determined by using both of light and electron ...2.0μg/ml ricin and left for one hour at RT, and the leaves of the tobacco growing plants were then inoculated as mentioned before in ...
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Isolation and Comparison of Galactose binding Lectins from Abris precatorius and Ricinus Communis pdf
... SUMMARY The agglutinins present in the seeds of Abrus precatorius and Ricinus communis have been separated from the toxins, abrin and ricin, and extensively purified.. The procedure invo[r] ...
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Chimeric Plantibody Passively Protects Mice against Aerosolized Ricin Challenge
... to ricin but not treated with c-PB10 experienced a rapid decrease in core body temperature and body weight, and then died within 48 h ...in ricin-challenged mice com- pared to control toxin-challenged mice ...
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Protein Biotoxins of Military Significance
... The Gram-positive pathogen Clostridium perfringens is a major cause of human and veterinary enteric disease largely because this bacterium can produce several toxins when present inside the gastrointestinal tract. C. ...
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Ricin: An Ancient Story for a Timeless Plant Toxin
... Kinetics of binding of the toxic lectins abrin and ricin to surface receptors 524. of human cells[r] ...
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Studies on the targeting and processing of proricin
... that ricin enzymatically inhibits protein synthesis (reviewed by Olsnes and Pihl, ...and ricin- treated rabbit reticulocyte ribosomes in a cell-free ...A chain action on the ribosomes. However, ...
287
Ricin, A Study of Purification and Properties of Kabat, Heidelberger, Bezer pdf
... By fractional precipitation with sodium sulfate it is possible to obtain a highly toxic protein Fraction Bl which is electrophoretically, ultracentrifugally, and immunochemically homogen[r] ...
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Propriétés et utilisation de l’huile de ricin
... de ricin en Inde, comme combustible dans les lampes a huile, en m edecine comme laxatif, mais aussi comme compos e de choix pour la gu erison des arthroses selon la m edecine ayurv ...au ricin dont elle a ...
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