RING-Type Ubiquitin Ligase
Characterization of a Novel RING Type Ubiquitin E3 Ligase GhRING2 Differentially Expressed in Cotton Fiber
... a RING-type ubiquitin ligase), and studied their expression in cotton fiber [20] ...the ubiquitin E3 ligase activity by an in vitro reconstitution assay ...four ...
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The structure and regulation of Cullin 2 based E3 ubiquitin ligases and their biological functions
... E3 ubiquitin ligase complexes; Cul2: Cullin-2; Rbx1: RING protein; ECS: Elongin B and C-Cul2 or Cul5-SOCS box protein; VHL: von Hip- pel–Lindau; HIFα: α subunits of hypoxia inducible factor; CH: ...
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The RING Finger Domain of Varicella-Zoster Virus ORF61p Has E3 Ubiquitin Ligase Activity That Is Essential for Efficient Autoubiquitination and Dispersion of Sp100-Containing Nuclear Bodies
... E3 ubiquitin ligase activity, which is required for autoubiquitination and targeting host proteins for proteasomal degradation, maps to its RING finger domain (1, 7) and, re- cently, a fragment of ...
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Human Papillomavirus Type 16 E7 Oncoprotein Associates with the Cullin 2 Ubiquitin Ligase Complex, Which Contributes to Degradation of the Retinoblastoma Tumor Suppressor
... The ubiquitin-proteasome pathway importantly contributes to regulated turnover of many cellular ...of ubiquitin to a target protein involves at least three enzymatic ...The ubiquitin-activating ...
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Putative E3 Ubiquitin Ligase of Human Rotavirus Inhibits NF κB Activation by Using Molecular Mimicry To Target β TrCP
... of type I and II IFNs, which signal in an autocrine and paracrine manner to restrict viral replication and spread ...a RING domain near its N terminus (32), which suggests that it acts as an E3 ...
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Herpes Simplex Virus Type 1 ICP0 Phosphorylation Mutants Impair the E3 Ubiquitin Ligase Activity of ICP0 in a Cell Type-Dependent Manner
... virus type 1 (HSV-1) infected cell protein 0 (ICP0) is a 110-kDa nuclear phosphoprotein that is required for both the efficient initiation of lytic infection and the reactivation of quiescent viral genomes from ...
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Rotavirus NSP1 Associates with Components of the Cullin RING Ligase Family of E3 Ubiquitin Ligases
... To investigate the ability of NSP1 to associate with the CRL3 complex during rotavirus infection, 293T cells were infected with the six different strains of rotavirus. At 9 h p.i., cells were harvested and lysates were ...
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Role of TRIM5α RING Domain E3 Ubiquitin Ligase Activity in Capsid Disassembly, Reverse Transcription Blockade, and Restriction of Simian Immunodeficiency Virus
... American Type Culture Collection and maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) and ...
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Ubiquitin Ligase Activities of Bombyx mori Nucleopolyhedrovirus RING Finger Proteins
... six RING finger proteins in BmNPV, and we showed that three of them, ...their RING finger ...the RING finger of RING-type E3 is a binding site for E2 enzymes (29, ...Many RING ...
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Contribution of E3-Ubiquitin Ligase Activity to HIV-1 Restriction by TRIM5αrh: Structure of the RING Domain of TRIM5α
... M ubiquitin aldehyde, a potent inhibitor of all ubiquitin C-ter- minal hydrolases, ubiquitin-specific proteases, and deubiquitylating enzymes ...wild- type TRIM5 ␣ rh were incubated in a ...
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Human Immunodeficiency Virus Type 1 Vif Induces Cell Cycle Delay via Recruitment of the Same E3 Ubiquitin Ligase Complex That Targets APOBEC3 Proteins for Degradation
... virus type 1 (HIV-1) encodes four accessory proteins, Vif, Vpr, Vpu, and Nef, which regulate multiple aspects of the host cell ...E3 ubiquitin ligases (9, 16, ...E3 ligase to target the cytidine ...
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The Poxviral RING Protein p28 Is a Ubiquitin Ligase That Targets Ubiquitin to Viral Replication Factories
... the RING domain of ICP0 catalyzed the formation of multiubiquitin adducts in the presence of UbcH5a, whereas GST was inactive ...the RING domain was truncated or lacked crucial cysteines ...multiple ...
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Genetic analysis of Vprbp in mice
... of ubiquitin-specific protease 1 (USP1) and may function to poise cells for repair during DNA replication (Terai et ...E3 ligase apparently causes monoubiquitylation of some substrates and ...
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Adenovirus E4 34k and E1b 55k Oncoproteins Target Host DNA Ligase IV for Proteasomal Degradation
... E3 ubiquitin ligase that mediates ubiquitination and proteasomal ...DNA ligase IV, which performs the joining step of NHEJ, is degraded as a consequence of adenovirus ...adenoviral ubiquitin ...
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Autographa californica Nucleopolyhedrovirus AC141 (Exon0), a Potential E3 Ubiquitin Ligase, Interacts with Viral Ubiquitin and AC66 To Facilitate Nucleocapsid Egress
... FIG 2 Coimmunoprecipitation of HA-AC141 and Myc-vUbi and impact of AC141 K87 mutations on BV production. (A) Sf9 cells were infected with 2 ⫻ KO-HA-ac141 ⫹ Myc-vubi, the WT, or vubiKO-Myc-vubi. Infected cells were ...
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The Human Immunodeficiency Virus Type 2 Vpx Protein Usurps the CUL4A-DDB1DCAF1 Ubiquitin Ligase To Overcome a Postentry Block in Macrophage Infection
... What predictions can be made regarding this restriction factor? First, it is conserved across several primate species based on our finding that Vpx from SIVsm can substitute for HIV-2 Vpx and on the reciprocal ...
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The ubiquitin ligase Cbl b limits Pseudomonas aeruginosa exotoxin T–mediated virulence
... by type III secretion directly into the host cell cytoplasm, resulting in disruption of host cell signaling and host innate ...host ubiquitin ligase Cbl-b interacts with the type III–secreted ...
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The KCTD family of proteins: structure, function, disease relevance
... mediates histone deacetylase (HDAC1) ubiquitination and degradation via cullin binding, resulting in reduced Hh/ Gli signaling [18]. The KCTD21 and KCTD6 have also been found to have the same features as KCTD11 [29]. ...
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Herpes simplex virus type 2 tegument protein UL56 relocalizes ubiquitin ligase Nedd4 and has a role in transport and/or release of virions
... HSV type 1 (HSV-1) UL56 has been shown to play an important role in pathogenicity in vivo [30,31], although little is known about its molecular ...anchored, type II membrane protein that is predicted to be ...
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Hippo Stabilises Its Adaptor Salvador by Antagonising the HECT Ubiquitin Ligase Herc4
... in ubiquitin conjugation and ubiquitin ...E3 ligase (Fig ...E3 ligase domain [53,54]. HECT domains bind specific ubiquitin-conju- gating enzymes (E2s), accept ubiquitin from the ...
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