RNase protection
Characterization of the Nonstructural Proteins of the Bocavirus Minute Virus of Canines
... and RNase protection assays (RPAs) were performed as pre- viously described ...homologous RNase protection probes were used in this ...
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The Epstein-Barr virus 3.5-kilobase latent membrane protein 1 mRNA initiates from a TATA-Less promoter within the first terminal repeat.
... LMP1 is essential for the Epstein-Barr virus (EBV)-induced growth transformation of normal B cells to immortalized lym- phoblastoid cell lines (LCLs) in vitro and is likely to be an important component of the ...
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Identification and Characterization of Two Internal Cleavage and Polyadenylation Sites of Parvovirus B19 RNA
... (B) RNase protection assays, performed as previously described (7, 12) using probe 4 (see ...the RNase protections shown in panel ...(E) RNase protection assays, performed as previously ...
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Assignment of the Multifunctional NS3 Protein of Bovine Viral Diarrhea Virus during RNA Replication: an In Vivo and In Vitro Study
... R, RNase protection of 500 ng of in vitro-transcribed DI9c RNA with antisense probe (positive control); 1, RNase protection performed with antisense and sense probes on BVDV DI9c cDNA ...
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Characterization of the major transcripts encoded by the regulatory MuDR transposable element of maize.
... Figure 5A shows RNase protection results for the splicing of each MuDR intron in an active Mutator stock containing multiple copies of MuDR (bzZ::mu9).. The RNA used fo[r] ...
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The Choice of Translation Initiation Site of the Rep Proteins from Goose Parvovirus P9-Generated mRNA Is Governed by Splicing and the Nature of the Excised Intron
... by RNase protection analysis, done as previously described [7] using the P1D probe [7] across the rep gene splice donor [data not shown]) by the introduction of an acceptor site mutation (CAACAG/AGCG to ...
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Transcriptional Analysis of Marek's Disease Virus Glycoprotein D, I, and E Genes: gD Expression Is Undetectable in Cell Culture
... Brunovskis and L. F. Velicar, Virology 206:324–338, 1995). Genes encoding the MDV glycoprotein D (gD), glycoprotein I (gI), and glycoprotein E (gE) homologs have been found in this region, although no gG homolog was ...
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Mutational analysis of the major homology region of Mason-Pfizer monkey virus by use of saturation mutagenesis.
... The protein composition of the mutant virions appeared to be normal, and an RNase protection experiment performed on viral particles released from COS-1 cells expressing wild-type and mu[r] ...
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The LIM homeodomain transcription factor Lmx1b plays a crucial role in podocytes
... Although by in situ hybridization VEGF mRNA can be detected in the podocytes of both wild-type e and Lmx1b–/– f mice, an RNase protection assay with 20 µg of total kidney RNA prepared fr[r] ...
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Dominant negative mutations of the scavenger receptor Native receptor inactivation by expression of truncated variants
... RNase protection assay of total RNA 5 tg from control nontransfected COS cells and from COS cells transfected with the native Type I bovine scavenger receptor, the mutant receptors trunc[r] ...
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Identification of intronic point mutations as an alternative mechanism for p53 inactivation in lung cancer
... The p53 gene initially was thought to be an oncogene, but recent evidence suggests that wild-type p53 can function as a tumor suppressor gene in lung, colon, and breast cancer as well as less common malignancies. This ...
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cis-active structural motifs involved in specific encapsidation of Moloney murine leukemia virus RNA.
... For RNase protection assays, viral and cellular RNAs were prepared from four 15-cm-diameter plates of confluent Psi2 cells expressing different virus constructs ...riboprobe RNase protection ...
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Differential regulation of human papillomavirus type 6 and 11 early promoters in cultured cells derived from laryngeal papillomas.
... sensitive RNase protection assay to simultaneously measure expression from the HPV E6, E7, and E1 promoters (P1, P2, and P3, respectively) in this manipulable culture system and found that P1, P2, and P3 ...
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Differential expression of Hz-1 baculovirus genes during productive and persistent viral infections.
... RNase protection experiments using fragment C + F as a probe indicated that all three virus-specific RNAs, the >9.5-, 6.6-, and CE 2.9-kb transcripts which were detected during productiv[r] ...
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The packaging phenotype of the SE21Q1b provirus is related to high proviral expression and not trans-acting factors.
... was used in each reaction, and reactions were carried out according to the manufacturer’s directions. Use of the neo probe (Fig. 2) indicated that, as expected, efficient packaging of genomic RNA requires an intact ...
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Vascular expression of extracellular superoxide dismutase in atherosclerosis
... Examination of macrophage expression of ecSOD. The above studies indicated that a novel form of ecSOD was ex- pressed in atherosclerotic vessels, and immunohistochemical staining indicated that it colocalized with ...
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B-Lymphocyte Proliferation during Bovine Leukemia VirusInduced Persistent Lymphocytosis Is Enhanced by T-Lymphocyte-Derived Interleukin-2
... an RNase protection assay to measure the expression of IL-2, IL-4, IL-10, and IFN-g mRNAs in ConA-stimulated PBMC isolated from persistently lymphocytotic BLV-infected, nonlymphocytotic BLV-infected, and ...
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Hereditary and acquired p53 gene mutations in childhood acute lymphoblastic leukemia
... the RNase protection assay and by single strand conformation polymorphism analysis in 23 of 25 ...of RNase protection was 50% (2 of ...
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Ventilation and oxygenation induce endothelial nitric oxide synthase gene expression in the lungs of fetal lambs
... from RNase protection assays and Western blot analysis were ana- lyzed densitometrically on a Macintosh computer (model 9500; Apple Computer, ...RNAase protection assays, to control for the amount of ...
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Reduced beta 1 receptor messenger RNA abundance in the failing human heart
... Heart failure in humans is characterized by alterations in myocardial adrenergic signal transduction, the most prominent of which is down-regulation of beta 1-adrenergic receptors. We tested the hypothesis that ...
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