RT qPCR
Identification of reference genes for RT-qPCR analysis in peach genotypes with contrasting chilling requirements.
... Five candidate reference genes were used in this study (Act, TEF2, RPII, UBQ10, and Tua5); these genes have been reported to be good potential candidates in previously published studies (Table 1). The primer efficiency ...
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Closed system RT-qPCR as a potential companion diagnostic test for immunotherapy outcome in metastatic melanoma
... multiplex RT-qPCR was performed using GeneXpert (GX) ...1-step RT- PCR and provides Ct values for the endogenous control, POLR2J , and the target genes, CD274 , PDCD1LG2 , CD8A and IRF1 ...
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Research Progress on Reference Genes of Insect for Quantitative Real time Reverse Transcription PCR (RT qPCR)
... efficiency. RT-qPCR has turned into a technique extensively employed for quantification of mRNA ...reason, RT-qPCR has been widely used in the expression profiling of selected genes in ...
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A novel mean centering method for normalizing microRNA expression from high throughput RT qPCR data
... Megaplex RT- qPCR platform, but is likely applicable to other high-throughput RT-qPCR-based ...singleplex RT-qPCR platforms, based on the identification of a small number of ...
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Reference gene selection for gene expression studies using RT qPCR in virus infected planthoppers
... of this conflictive gene, all three approaches clearly separated the 7 candidate genes in two distinct groups according to their stability indexes: one group of a low variability and high stability composed of UBI, ACT, ...
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The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling
... In a previous study, altered gene expression in human lung epithelial cells (BEAS-2B) was observed after expo- sure to ZnO NPs of 20 nm [21]. It was found that sub- lethal concentrations of ZnO were able to increase the ...
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Evaluation of reference genes for RT-QPCR studies in the seagrass Zostera muelleri exposed to light limitation
... via RT-qPCR, it is critical to normalize the levels of target genes on the basis of reference genes that are known to be continuously expressed at the same levels, to control for the amount and quality of ...
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Reference Genes for RT qPCR Analysis of Environmentally and Developmentally Regulated Gene Expression in Alfalfa
... in RT-qPCR analysis of gene expression in ...When RT-qPCR data were normalized with stably expressed genes, ob- servations on the expression of genes functionally related to cold acclimation ...
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An ex vivo RT-qPCR-based assay for human peripheral leukocyte responsiveness to glucocorticoids in surgically induced inflammation
... Patients and methods: Changes in expression of the GC-regulated genes GILZ, IL1R2, FKBP5, and HLA-DR and glucocorticoid receptor alpha (GRα) were determined by reverse transcriptase quantitative polymerase chain reaction ...
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<p>MIR22HG As A Tumor Suppressive lncRNA In HCC: A Comprehensive Analysis Integrating RT-qPCR, mRNA-Seq, And Microarrays</p>
... in-house RT-qPCR, RNA-sequen- cing, microarray, and literature studies to conduct a comprehensive evaluation of the clinico- pathological and prognostic signi fi cance of MIR22HG in an extremely large group ...
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Original Article Detecting T315I BCR-ABL mutants in leukemia between RT-qPCR and conventional Sanger sequencing: a comparative study
... that gene mutations of BCR-ABL are common in leukemia patients, especially in patients who are insensitive to most current treatments. In the present study, we utilized two strategies, RT-qPCR and Sanger ...
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Robustness of biomarker determination in breast cancer by RT-qPCR: impact of tumor cell content, DCIS and non-neoplastic breast tissue
... the RT-qPCR signal is dominated by the invasive tumor component, allowing for consistent cal- culations in whole sections with up to 60% DCIS and in a TCC range of ...
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Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M leprae from nasal swab samples
... In our setting, a viability assay should serve two purposes. First, there was the need for a diagnostic tool to monitor the treatment response of leprosy pa- tients attending our outpatient clinic in Munich, Germany. ...
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Selection of reference genes from two leafhopper species challenged by phytoplasma infection, for gene expression studies by RT qPCR
... on RT-qPCR quantification of mRNA levels are extensively used in different research fields, but reliability of such an analysis depends on the use of one or more stably expressed housekeeping genes, as ...
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Glycophenotyping of osteoarthritic cartilage and chondrocytes by RT qPCR, mass spectrometry, histochemistry with plant/human lectins and lectin localization with a glycoprotein
... from RT-qPCR profiling, the glycan popula- tion detected includes core-substituted, α2,3/6-sialylated N-glycans, with the presence of LacdiNAc-terminated structures and core 2 ...
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Evaluation of reference genes for RT-qPCR study in abalone Haliotis discus hannai during heavy metal overload stress
... To evaluate the expression stability of the HKGs tested, the RT-qPCR data was statistically analyzed using different types of software programs, geNorm (qBase- PLUS ver. 3.0) (Vandesompele et al. 2002; ...
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Evaluation of reference genes for normalizing RT-qPCR in leaves and suspension cells of Cephalotaxus hainanensis under various stimuli
... Sequences of candidate reference genes (Additional file 5) were mined from our full-length transcriptome database obtained by the SMRT (Single-Molecule Real-Time) technology on PacBio Sequel platform (Unpublished, ...
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Clinical value of miR 198 5p in lung squamous cell carcinoma assessed using microarray and RT qPCR
... in-house RT-qPCR ...and RT-qPCR data indicated that miR-198-5p might be a biomarker of LUSC, but the sensitivity and spe- cificity were not sufficiently ...
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Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells
... the RT-qPCR amplification region for assessing the efficacy of the siRNA target gene knockdown and that this finding can be extended to other mRNAs in lung cancer ...that qPCR primers for siRNA work ...
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Detection of siRNA induced mRNA silencing by RT qPCR: considerations for experimental design
... Validated siRNA duplexes directed to protein kinase C-epsilon (PKCε) mRNA were used to knockdown PKCε expression in HDMEC. Two separate duplexes were used targeting separate regions of the target mRNA (figure 1A-B). A ...
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