RT-QUIC
Transmission studies of chronic wasting disease to transgenic mice overexpressing human prion protein using the RT-QuIC assay
... As the number of CWD-infected cervids continues to increase so does the potential for human exposure to cervid tissues containing CWD prions. Understand- ing the risk CWD may pose to human health is critical to avoid a ...
14
Efficient RT QuIC seeding activity for α synuclein in olfactory mucosa samples of patients with Parkinson’s disease and multiple system atrophy
... that RT-QuIC is highly ...Since RT-QuIC analyses of brain homogenates enabled us to identify PD and MSA samples, we decided to verify whether this dis- crimination could also occur in ...
14
Rapid and ultra-sensitive quantitation of disease-associated α-synuclein seeds in brain and cerebrospinal fluid by αSyn RT-QuIC
... αSyn RT-QuIC is capable of detecting sub- femtogram amounts of rαSyn fibrils that are orders of magnitude less than those required for more conven- tional assays such as Western blotting and ELISA, with ...
10
Preparation of lyophilized recombinant prion protein for TSE diagnosis by RT QuIC
... as a seed to initiate conversion of the provided substrate from a monomeric prion protein to an oligomeric form. RT-QuIC uses bacterially expressed, purified recom- binant prion protein (rPrP) and ...
6
Rapid and Sensitive RT QuIC Detection of Human Creutzfeldt Jakob Disease Using Cerebrospinal Fluid
... the RT-QuIC results from the sCJD (red) and non-CJD (orange) ...weaker RT-QuIC responses than those obtained with the new conditions ...positive RT-QuIC tests from 46/48 sCJD ...
7
Detection and Discrimination of Classical and Atypical L Type Bovine Spongiform Encephalopathy by Real Time Quaking Induced Conversion
... our RT-QuIC test at least as sensitive as infectivity bioassays ...on RT-QuIC substrate provides a prototypic example of how prion strains can have different seeding activities that may, in ...
6
Infectious Prions in the Pregnancy Microenvironment of Chronic Wasting Disease-Infected Reeves' Muntjac Deer
... and RT-QuIC. In this study, we have demonstrated RT-QuIC seeding activity in uterus, placentome, ovary, and amniotic fluid but not in allantoic fluids harvested from CWD-infected Reeves’ muntjac ...
15
PAD Beads enrichment enhances detection of PrPSc using real time quaking induced conversion
... RT-QuIC reactions were performed and analyzed as previously described [23–25]. The reaction substrate (98 µl) was composed of 10 mM phosphate buffer (pH 7.4), 300 mM NaCl, 0.1 mg/ml recombinant bank vole ...
7
Modified Protein Misfolding Cyclic Amplification Overcomes Real Time Quaking Induced Conversion Assay Inhibitors in Deer Saliva To Detect Chronic Wasting Disease Prions
... CWD prions have been previously identified in excreta, including saliva, feces, and urine (7, 10, 13, 23). While the contribution of each of these shed prion sources remains unknown, comparisons of prion concentration in ...
15
Antemortem Detection of Chronic Wasting Disease Prions in Nasal Brush Collections and Rectal Biopsy Specimens from White Tailed Deer by Real Time Quaking Induced Conversion
... the RT-QuIC assay in the present study (⬃90 to 94%) was lower than anticipated in both RAMALT biopsy spec- imens and nasal brush collections and lower than that in our pre- vious analyses of ...while ...
9
Longitudinal Detection of Prion Shedding in Saliva and Urine by Chronic Wasting Disease-Infected Deer by Real-Time Quaking-Induced Conversion
... Positive RT-QuIC wells were deemed positive when the thioflavin T fluorescence reached a value that was 5 standard deviations higher than the initial fluorescence ...
10
Insights into Chronic Wasting Disease and Bovine Spongiform Encephalopathy Species Barriers by Use of Real-Time Conversion
... we used circular dichroism (CD) spectroscopy (Fig. 1B to E). All substrates had minima at 208 nm and 222 nm, indicating a pre- dominance of ␣-helical secondary structure, as expected. Each preparation of recombinant ...
8
Establishing quantitative real-time quaking-induced conversion (qRT-QuIC) for highly sensitive detection and quantification of PrPSc in prion-infected tissues
... In a recent study, Wilham and colleagues [7] used SD50 (50% seeding dose) to quantify seeding activity in the RT-QuIC. SD50 was defined as minimum seeded amount causing 50% of RT-QuIC ...
9
Seeded Amplification of Chronic Wasting Disease Prions in Nasal Brushings and Recto anal Mucosa Associated Lymphoid Tissues from Elk by Real Time Quaking Induced Conversion
... of RT-QuIC would be comparable to that of IHC analysis of RAMALT and would correspond to that of IHC analy- sis of postmortem ...between RT-QuIC and IHC ...that RT-QuIC, like IHC ...
10
Detection of Chronic Wasting Disease in the Lymph Nodes of Free Ranging Cervids by Real Time Quaking Induced Conversion
... standardized RT-QuIC seeded amplification assay with two different fluorophores (thio- flavin T [ThT] and thioflavin S [ThS]) to examine, in a blinded manner, retropharyngeal lymph node (RLN) samples ...
7
Conformational Properties of Prion Strains Can Be Transmitted to Recombinant Prion Protein Fibrils in Real-Time Quaking-Induced Conversion
... RT-QUIC product analysis. For detection of protease-resistant rPrP, 10 l of the QUIC samples (1 g of rPrP) was diluted with 40 l of buffer (300 mM NaCl, 50 mM HEPES, pH 7.5) and digested with ...
11
Participants’ understanding of a randomized controlled trial (RCT) through informed consent procedures in the RCT for breast cancer screening, J START
... The present study aimed to evaluate the degree of par- ticipants’ understanding of an RCT and investigate the associated factors. We administered questionnaires to 376 healthy Japanese women on the day of enrolment at ...
10
Comparing MALDI-ToFMass Spectrometry with Molecular and Biochemical Methods in Identifying Enterococcus Faecium and Enterococcus FaecalisIsolated from Clinical Samples
... Conclusion: According to our results, MALDI-TOF mass spectrometry in comparison with Molecular and Biochemical Methods could be a reliable and accurate method that can easily and quic[r] ...
7
Endoscopic paranasal sinus surgery: radiographic evaluation of severe complications
... ethmoid, Rt defect plate, contrast in Rt repair ethmoid Craniotomy, Rt defect Bony defect, Rt crib plate, contrast in Rt repair ethmoid Craniotomy, Lt defect reBony defect, Lt crib plate[r] ...
7
Some factors affecting the activation of virus preparations made from tobacco leaves infected with a tobacco necrosis virus
... When preparations of the Rothamsted tobacco necrosis virus made in 1945 were ultracentrifuged they gave pellets that were crystalline ; when these pellets were washed quic[r] ...
19