Sandwich ELISA
Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase
... Different mab-α and GAM-AP antibodies concentra- tions were used in the experiment. Generated fluorescent signals proved to be close to the maximum ones ever observed for the bead-based fluorescent assay irrespec- tive ...
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Quantification of Pla or 3, a Platanus orientalis Allergen, Grown under Different Environmental Conditions, by Sandwich ELISA
... A sandwich ELISA was developed and validated to quantify Pla or 3 in pollen ...validated ELISA, we showed a substantial difference between the amounts of Pla or 3 in pollens grown in different ...
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Copper Ions Enhance Signal Intensity of Sandwich ELISA for Amorphous Aggregates of Amyloid β42
... [13]. Sandwich ELISA using these two antibodies resulted in the highly sensitive detection of soluble ...the sandwich ELISA was shown to increase through the ad- dition of 50 μM Cu 2+ ; ...
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Dual monoclonal antibody-based sandwich ELISA for detection of in vitro packaged Ebola virus
... Background: Rapid transmission and high mortality of Ebola virus disease (EVD) highlight a urgent need of large scale, convenient and effective measure for Ebola virus screening. Application of monoclonal antibodies ...
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Antibody-sandwich ELISA analysis of a novel blood biomarker of CST4 in gastrointestinal cancers
... In order to evaluate the clinical detection efficacy of the established antibody-sandwich ELISA analysis system for CST4, another 588 serum samples (Table 1) were used. The concentration ranges of CST4 ...
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Detection of Coproantigens by Sandwich ELISA in Rabbits Experimentally Infected with Fasciola gigantica.
... Rapid diagnostic methods would facilitate control of the parasite. Immunoassays as diag- nostic tools can be employed either by anti- body detection in the infected serum (1) or by antigen detection (2). The time ...
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Accurate and Sensitive Quantification of Soy Proteins in Raw and Processed Food by Sandwich ELISA
... Results for processed matrices in comparison to unprocessed original material are shown in table 7. For hazelnut cake, recoveries decrease slightly with increasing temperature and time but are still acceptable for this ...
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Development and Application of a Double-Antigen Sandwich Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Porcine Circovirus 2
... Optimization of the working conditions of the double-antigen sandwich ELISA. The optimum concentrations of coating antigen and HRP-conjugated antigens and the ratio of HRP-conjugated antigens to the testing ...
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THE ROLE OF ESTIMATION OF BETA –D-GLUCAN IN EARLY DIAGNOSIS OF FUNGEMIA IN FEBRILE NEUTROPENIC PATIENTS.
... the Sandwich ELISA assay, the receiver operating characteristic (ROC) curve was constricting to assess the accuracy of serum βDG levels in discrimination between patients with or without fungemia, by ...
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The development and use of an ELISA-based method to follow the distribution of cellulase monocomponents during the hydrolysis of pretreated corn stover
... A simple, high-throughput assay that can specifically fol- low and quantify individual enzymes present in the com- plex enzyme mixtures that are used to hydrolyse pretreated lignocellulosic substrates was developed and ...
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Dynamics of Multicellular Aggregation and Disaggregation: Implications for Tissue Engineering and Cancer Metastasis
... E-cadherin:β-catenin ELISA or (B) sandwich ...complex ELISA was normalized to either total cellular levels of E- cadherin or β-catenin as measured by the sandwich ELISA for both ...
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Identification of Secreted Antigen 3 from Babesia gibsoni
... double-antibody sandwich ELISA, the native BgSA1 could be detected in the plasma of an asplenic dog infected with ...wich ELISA to determine whether the antigen in plasma can reflect the infection ...
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Quantitative immunoassay for mink immunoglobulin in serum and milk
... a sandwich ELISA for quantifying IgG in mink serum and milk was ...the ELISA was elucidated by investigating its reactivity with full mink serum as well as mink milk by Western ...during ELISA ...
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... Several antigen detection assays with diverse sensitivity and specificity have been utilized for the diagnosis of HF, based on detection of Fasciola Circulating Antigens (CAs) or coproantigens. The principal approach for ...
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Detection of Antigen in Sera of Patients with Invasive Aspergillosis: Intra and Interlaboratory Reproducibility
... the ELISA result is suffi- cient, since a test that detects antigen in the serum is usually only one of several diagnostic tests or procedures which are used to make the ...the ELISA reactivities of two ...
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Detection of Cell Wall Mannoprotein Mp1p in Culture Supernatants of Penicillium marneffei and in Sera of Penicilliosis Patients
... the ELISA is high, about 20 ...a sandwich ELISA for the detection of circulating galactomannan in patients with invasive aspergillosis ...antigen ELISA described here is also ...
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Monoclonal antibody based enzyme linked immunosorbent assays for identification and serotyping of Vibrio cholerae O1
... In the sandwich ELISA, a monoclonal antibody against the group-specific antigen was used as capture antibody, whereas peroxidase-conjugated monoclonal antibodies directed against groupan[r] ...
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PADI4 and tumourigenesis
... and ELISA showed that the citrullination of AT decreased its ability to inhibit thrombin ...Using sandwich ELISA, we detected higher levels of PADI4 and citrullinated AT levels in the plasma of ...
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Investigations for the Possible Use of a Monoclonal Antibody Produced against Strongyloides ratti Antigen as an Immunodiagnostic Reagent for Active Strongyloidiasis
... Methods: The study was conducted in the Department of Medical Microbiology and Parasitology, University Putra Malaysia in 2014-2017. Saline extract protein from the infective larvae of S. ratti was used to immunize ...
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Evaluation of the clinical value of ELISA based on MPT64 antibody aptamer for serological diagnosis of pulmonary tuberculosis
... this ELISA method may be suitable for the serological diagnosis of spu- tum smear negative ...Since sandwich ELISA is a rapid, simple and very common test used by many labora- tories and laboratory ...
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