Sea Urchin Embryos
Ca(2+) in specification of vegetal cell fate in early sea urchin embryos
... Fig. 3. Electrical coupling in 16- to 60-cell stage sea urchin embryos of Paracentrotus lividus. To measure electric communication, two patch pipettes in the whole-cell voltage-clamp configuration ...
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Transcriptional regulation of the gene for epidermal growth factor like peptides in sea urchin embryos
... in sea urchin embryos are expressed rather evenly in either the oral or the aboral ectoderm, and their expression seems to be dependent on the lineage of the embryonic cells (Davidson, ...prism ...
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ERK1 activation is required for S phase onset and cell cycle progression after fertilization in sea urchin embryos
... In order to study the pattern of GFP-PCNA accumulation during the first cell cycle, we microinjected recombinant GFP- PCNA protein to a concentration of 60 nM. Fig. 1B illustrates the accumulation of chimera during S ...
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The Regulatory Origin of Oral and Aboral Mesoderm in Sea Urchin Embryos
... in sea urchin were aimed at finding all regulatory genes that are active during early development (Howard-Ashby et ...collected sea urchin embryos at hourly intervals and determined ...
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Specification of ectoderm restricts the size of the animal plate and patterns neurogenesis in sea urchin embryos
... The larval nervous system is an array of neurons that control swimming and feeding. There are several clusters of neurons with associated neuropil that are thought to function as ganglia. The best know of these is the ...
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Cost, effectiveness and environmental relevance of multidrug transporters in sea urchin embryos
... the sea urchin embryos used here, are typically considered to have greater metabolic rates than later life stages (Leong and Manahan, ...stage embryos used in this study is actually quite low ...
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ABCC5 is required for cAMP mediated hindgut invagination in sea urchin embryos
... in sea urchin ...knockdown embryos gastrulate, but ∼ 90% develop a prolapse of the hindgut by the late prism stage ( ∼ 8 h after C5a protein expression normally ...knockdown embryos, and ...
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Late Alk4/5/7 signaling is required for anterior skeletal patterning in sea urchin embryos
... VEGF is a spatially restricted ectodermal cue that is required both for normal PMC positioning and for skeletogenesis. Zygotic inhibition of VEGF blocks skeleton formation entirely and disrupts the formation of the ...
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Exposure to ultraviolet radiation causes apoptosis in developing sea urchin embryos
... in sea urchin embryos and larvae (Voronina and Wessel, 2001; Roccheri et ...green sea urchin embryos reveal a significant increase in the number of apoptotic cells after exposure ...
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A marine diatom derived aldehyde induces apoptosis in copepod and sea urchin embryos
... in sea urchin embryos treated with DD using a commercially available kit designed for the mammalian version of ...in sea urchin embryos 60·min after addition of ...
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p38 MAPK is essential for secondary axis specification and patterning in sea urchin embryos
... In sea urchin embryos, the expression of Nodal on the oral (ventral) side of the embryo is the first known molecular determinant of the oral-aboral axis (the embryonic dorsoventral axis), and is ...
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Neurogenesis in sea urchin embryos and the diversity of deuterostome neurogenic mechanisms
... the first neurons to differentiate is likely to occur in late cleavage. Previous studies have established that ectoderm differentiates in distinct domains in which TGF β signaling initiates gene regulatory networks that ...
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Fixed metabolic costs for highly variable rates of protein synthesis in sea urchin embryos and larvae
... in sea urchin embryos do differ from protocols that use a ‘flooding dose’ of labeled amino acid, rather than a ‘tracer ...and sea urchin embryos specifically, have very high ...
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Notch signaling patterns neurogenic ectoderm and regulates the asymmetric division of neural progenitors in sea urchin embryos
... in embryos in which CRISPR/Cas9 was used to mutate Sp- ...in sea urchin embryos shares many of the features common to neurogenesis in all bilaterians (Hartenstein and Stollewerk, ...In ...
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Development of a dopaminergic system in sea urchin embryos and larvae
... In sea urchin embryos, motile cilia are evident from the blastula stage, and rotatory movement by embryos can be observed in the fertilization ...instance, sea urchin blastulae ...
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I Studies on alkaline phosphatase activity in developing sea urchin embryos II Studies on the activation of protein biosynthesis in sea urchin eggs at fertilization
... Hist.ochemical studies of alkaline phosphatase in developing sea urchin eggs indicated that the enzyme was distributed uniformly throughout the unfertilized and ~ertilized eggs, and clea[r] ...
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The control of foxN2/3 expression in sea urchin embryos and its function in the skeletogenic gene regulatory network
... the embryos showed the albino phenotype as expected from previous reports using these reagents (Croce and McClay, 2010; Sherwood and McClay, 1999; Sweet et ...
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BEATING OF CILIA OF SEA URCHIN EMBRYOS: A CRITICAL COMPARISON OF THE NORMAL AND REVERSED BEATING OF CILIA OF ISOLATED CELLS
... In this paper, ciliary movement was investigated in normal and reversed beating recorded from cells isolated from echinoplutei. Isolation of the cells may change the viscous load on the beating cilium, because the cell ...
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Spblimp1/krox: A transcriptional regulator with a central role in endomesoderm specification in sea urchin embryos
... functions of the foxa gene. The foxa gene is expressed in the endoderm, as gastrulation proceeds, primarily in the foregut and midgut. Perturbation experiments with alpha -foxa MASO resulted in a sharp increase in target ...
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Mitochondria and the development of sea urchin embryos
... comparable levels of protein synthesis in artificially activated non-nucleate fragments and fertilized eg g s {8-10, 34 & 35 and the studies showing continued protein synthesis in the pr[r] ...
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