secretion system
A Novel Mechanism for Protein Delivery by the Type 3 Secretion System for Extracellularly Secreted Proteins
... ABSTRACT The type 3 secretion system (T3SS) is essential for bacterial virulence through delivering effector proteins directly into the host cytosol. Here, we identi- fied an alternative delivery mechanism ...
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Identification of Mammalian Proteins That Collaborate with Type III Secretion System Function: Involvement of a Chemokine Receptor in Supporting Translocon Activity
... ABSTRACT The type III secretion system (T3SS) is a highly conserved protein delivery system found in multiple Gram-negative pathogens, including Yersinia pseudotuberculosis. Most studies of Yersinia ...
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The Tip of the VgrG Spike Is Essential to Functional Type VI Secretion System Assembly in Acinetobacter baumannii
... ABSTRACT The type VI secretion system (T6SS) is a critical weapon in bacterial war- fare between Gram-negative bacteria. Although invaluable for niche establishment, this machine represents an energetic ...
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Engineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein.
... directed secretion to the extracellular space via strategies that make use of ‘leaky strains’ [15], where heterologous proteins are fused to a secretion tag (for example ompA, ompC, pelB [16, 17]), to ...
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Secretome Analysis of Vibrio cholerae Type VI Secretion System Reveals a New Effector Immunity Pair
... VI secretion system (T6SS) is a dynamic macromolecular organelle that many Gram-negative bacteria use to inhibit or kill other prokaryotic or eukaryotic ...
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Control of Type III Secretion System Effector/Chaperone Ratio Fosters Pathogen Adaptation to Host Adherent Lifestyle
... IMPORTANCE Host colonization by extracellular pathogens often entails the transi- tion from a planktonic lifestyle to a host-attached state. Enteropathogenic E. coli (EPEC), a Gram-negative pathogen, attaches to the ...
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Self Labeling Enzyme Tags for Analyses of Translocation of Type III Secretion System Effector Proteins
... The choice of SLE appears to be of critical importance. We found that HaloTag fusions are functional for effector proteins of the SPI2-T3SS but not for those of the SPI1-T3SS or the Yersinia T3SS. The opposite was ...
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The Citrobacter rodentium type III secretion system effector EspO affects mucosal damage repair and antimicrobial responses.
... by secretion of IL-22, in the absence of which mice succumbed to the ...III secretion system (T3SS) that injects effectors into colonic intestinal epi- thelial cells ...as secretion of IL-22 ...
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Structural Basis of Type 2 Secretion System Engagement between the Inner and Outer Bacterial Membranes
... protein secretion. In Gram-negative bacteria, the type 2 secretion system (T2SS) is composed of a pseudopilus assembly platform in the inner membrane and a secretin complex in the outer ...
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The architecture of EssB, an integral membrane component of the Type VII secretion system
... It is intriguing that encoded on the T7SS gene cluster is EssC, a large, membrane-bound ATPase. The ATPase activity resides on the C-terminal part of the protein and can provide the motive force for the T7SS. The ...
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Peptidomimetic Small Molecules Disrupt Type IV Secretion System Activity in Diverse Bacterial Pathogens
... IL-8 secretion and NF-B activation by C10 and KSK85 prompted us to measure CagA de- livery to gastric epithelial ...and secretion of IL-8 ...V secretion system- mediated release of the ...
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Extracellular secretion of a recombinant therapeutic peptide by Bacillus halodurans utilizing a modified flagellin type III secretion system
... Our system utilizes an endogenous promoter and there- fore no inducers need to be added to the ...determine secretion levels obtained from either the Salmonella or ...coli secretion system ...
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Enterococcus faecalis Enhances Expression and Activity of the Enterohemorrhagic Escherichia coli Type III Secretion System
... The mechanism underlying enhanced LEE transcription in the presence of B. thetaiotaomicron has been described previously (6); however, the mechanism under- lying the effect of E. faecalis on LEE transcription is unknown, ...
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Postreplication Roles of the Brucella VirB Type IV Secretion System Uncovered via Conditional Expression of the VirB11 ATPase
... III secretion system gene icsA has revealed a postinvasion role of the ...III secretion system in the bacterium’s intercellular spread ...
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Structural insights into the function of type VI secretion system TssA subunits.
... The type VI secretion system (T6SS) is a multi-protein complex that injects bacterial effector proteins into target cells. It is composed of a cell membrane complex anchored to a con- tractile bacteriophage ...
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Negative Autogenous Control of the Master Type III Secretion System Regulator HrpL in Pseudomonas syringae
... ABSTRACT The type III secretion system (T3SS) is a principal virulence determi- nant of the model bacterial plant pathogen Pseudomonas syringae. T3SS effector proteins inhibit plant defense signaling ...
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Migration of Type III Secretion System Transcriptional Regulators Links Gene Expression to Secretion
... unaltered secretion profile of HrpA2, HrpZ1 showed dramatic differences in its extracellular and intracellular detection for wild-type ...a secretion- incompetent mutant. The absence of HrpZ1 ...
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Engineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein
... prototype secretion construct ...in secretion efficiency ...protein secretion assay was carried out ...the secretion construct (SC1) ...
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Dynamics of the Type III Secretion System Activity of Enteropathogenic Escherichia coli
... III secretion systems (TTSSs) are utilized by many patho- genic bacteria, including enteropathogenic Escherichia coli (EPEC) and Salmonella enterica serovar Typhimurium, to trans- locate effector proteins into ...
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Topological Analysis of the Type 3 Secretion System Translocon Pore Protein IpaC following Its Native Delivery to the Plasma Membrane during Infection
... 3 secretion sys- tem (T3SS) to deliver effector proteins into eukaryotic ...initiates secretion of the two bacterial proteins that assemble into the translocon pore in the plasma ...protein secretion ...
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