Selectable marker
Utility of the pat gene as a selectable marker gene in production of transgenic Dunaliella salina
... Background: The objective of this study was to develop an efficient selectable marker for transgenic Dunaliella salina. Results: Tests of the sensitivity of D. salina to the antibiotic chloramphenicol and ...
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Nourseothricin N-acetyl transferase (NAT), a new selectable marker for nuclear gene expression in Chlamydomonas
... a selectable marker has been used in microal- gae but so far only in marine diatoms including Chaetoc- eros gracilis, ...ble marker in a fresh water green alga, opening a promis- ing prospect in ...
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Determining the optimal concentration of mannose as an effective selection agent for transformed oil palm cells using the phosphomannose isomerase (pmi) gene as a positive selectable marker
... negative selectable marker genes. The negative selectable marker genes used in oil palm transformation are genes that confer resistance to the antibiotic hygromycin, hygromycin ...
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Genetic Mapping Reveals that Sinefungin Resistance in Toxoplasma gondii Is Controlled by a Putative Amino Acid Transporter Locus That Can Be Used as a Negative Selectable Marker
... genome sequencing of the progeny and subsequent identification of single nucleotide polymorphisms (SNPs) inherited from the parents. Based on this high-density genetic map, we were able to pinpoint the sinefungin ...
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A selectable marker allows investigation of a nontransforming Epstein-Barr virus mutant.
... Transfection of the T1E2-HYG cosmid or the T1E2 parent cosmid lacking SVHYG into P3HR1 also yielded similar numbers of primary B-lymphocyte transforming recombinants, thereby indicating [r] ...
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Plaque size phenotype as a selectable marker to generate vaccinia virus recombinants.
... 3A, immunoblots reacted with monoclonal antibody C3, which is specific for the 14K protein 14, 15, revealed a single polypeptide of 14K in cells infected with wild-type virus lane 1, a 1[r] ...
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Utility of I SceI and CCR5 ZFN nucleases in excising selectable marker genes from transgenic plants
... in the same transgenic line as this mechanism destroys the target site by creating insertion–deletions (indels). Several nucleases, including meganucleases, ZFN, and CRISPR/Cas have been used for creating concomitant ...
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Chromosomal Integration Pattern of a Helper-Dependent Minimal Adenovirus Vector with a Selectable Marker Inserted into a 27.4-Kilobase Genomic Stuffer
... lian cell lines and were performed under very similar experi- mental conditions (27, 62). As discussed above, the several- fold-enhanced apparent integration frequency of the minimal adenovirus vector possibly gives a ...
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Exceptional Segregation of a Selectable Marker (KanR) in Arabidopsis Identifies Genes Important for Gametophytic Growth and Development
... Further, DEROLES and GARDNER (1988) examined selfed and backcrossed progeny from 104 primary trans- formants of Petunia and found that 55 segregated for a deficiency of [r] ...
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Expression of Hygromycin Phosphotransferase Alters Virulence of Histoplasma capsulatum
... dominant selectable marker used in the molecular manipulation of ...few selectable markers have been charac- terized ...able marker in the generation of knockout strains of ...a marker ...
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Identification of the Orthopoxvirus p4c Gene, Which Encodes a Structural Protein That Directs Intracellular Mature Virus Particles into A-Type Inclusions
... Shown are sections of cells infected with vaccinia virus A504 p4c::gpt ati1 containing a p4c gene disrupted by the insertion of a selectable marker gene, the gpt gene A; vaccinia virus A[r] ...
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A novel Gateway®-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciens
... In order to construct a new Gateway ® -compatible desti- nation binary vector that can be used for selection of recombinant plasmids in A. tumefaciens, the SacB-SacR gene cassette was amplified and cloned into the BbvCI ...
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Modifying fatty acid profiles through a new cytokinin-based plastid transformation system
... To demonstrate proof of principle, two additional chloro- plast expression vectors, pP1016-KasIII (Figure 2a) and pPS59a-KasIII (Figure 2b), were designed to determine whether the ipt selection system may be used for ...
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Advances in Production of Marker Free Transgenic Plants: Current Challenges and Future Perspectives
... lethal marker gene by Mendelian ...having selectable marker gene while the other smaller T-DNA having gene of ...selection marker gene on the longer T-DNA while keeping targeting gene on the ...
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A versatile toolkit for high throughput functional genomics with Trichoderma reesei
... An overnight culture (200 rpm, 30°C) of the yeast strain WW-YH10 was prepared. 1 ml of the overnight culture was added to 50 ml of fresh YPD (1% yeast extract, 2% peptone, 1% glucose (all chemicals from Sigma Aldrich, ...
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Stable Growth of Wild-Type Hepatitis A Virus in Cell Culture
... a selectable marker irrespective of the presence of the main cell culture-adapting mutation at position ...Bsd selectable marker (HAV8Y-Bsd and ...
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CoIN: co-inducible nitrate expression system for secondary metabolites in Aspergillus nidulans
... Next, we set out to test which of the 25 stc gene pro- moters would be nitrate-inducible in our production strain (TPMW2.3). Since TPMW2.3 is a uracil/uridine and riboflavin auxotroph, we designed plasmids that contain ...
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Repeated use of GAL1 for gene disruption in Candida albicans.
... We have explored the feasibility of using a method described by ALANI, GAO and KLECKNER (1987). In this procedure, the selectable marker is flanked by direct repeats o[r] ...
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Calcineurin Controls Growth, Morphology, and Pathogenicity in Aspergillus fumigatus
... Here we show that gene replace- ment of the calcineurin A ( cnaA ) locus with an auxotrophic selectable marker led to severe defects in the growth and development of the fungus. These de[r] ...
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Embryonic Lethality and Tumorigenesis Caused by Segmental Aneuploidy on Mouse Chromosome 11
... negative selectable marker is targeted to the region of At this time, we cannot discriminate if this lethality is interest in a hybrid ES cell line, the cells are irradiated, caused by a single gene or by ...
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