Semliki Forest virus
The 6-kilodalton membrane protein of Semliki Forest virus is involved in the budding process.
... two virus transmembranal glycoproteins which form the spikes of the infectious ...in virus replication and structure has been studied by use of a variety of mutationally altered forms of 6K, which yield ...
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Furin Processing and Proteolytic Activation of Semliki Forest Virus
... alphavirus Semliki Forest virus (SFV) infects cells via a low-pH-dependent membrane fusion reaction mediated by the E1 envelope ...of virus fusion and ...unprocessed virus produced in ...
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Mutations in the putative fusion peptide of Semliki Forest virus affect spike protein oligomerization and virus assembly.
... of Semliki Forest virus (SFV) form a heterodimeric complex in the rough endoplasmic ...and virus budding take ...C, virus spike proteins containing either mutation were efficiently ...
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In vivo depletion of CD8+ T cells prevents lesions of demyelination in Semliki Forest virus infection.
... 7629 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Following intraperitoneal infection of BALB/c mice with the A774 strain of Semliki Forest virus, the virus spreads t[r] ...
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Attenuation of Semliki Forest Virus Neurovirulence by MicroRNA-Mediated Detargeting
... liver. Semliki Forest virus (SFV) is a positive-strand RNA virus and, similar to the related alphaviruses, like Sindbis virus, has potential as a gene therapy vector and an oncolytic ...
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Deletion Analysis of a Defective Interfering Semliki Forest Virus RNA Genome Defines a Region in the nsP2 Sequence That Is Required for Efficient Packaging of the Genome into Virus Particles
... of Semliki Forest virus (SFV) defective interfering (DI) RNA 19 (DI-19) is coterminal with the infectious genome and contains two major ...DI virus particles that had been released into the ...
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Infectious particles derived from Semliki Forest virus vectors encoding murine leukemia virus envelopes.
... Semliki Forest virus (SFV) contains a single-stranded, ; 11-kb positive-sense RNA, the first two-thirds of which en- codes nonstructural proteins involved in viral replication, while the last third ...
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Biogenesis of the Semliki Forest Virus RNA Replication Complex
... of Semliki Forest virus (SFV) RNA polymerase, were localized in infected cells by confocal microscopy using double labeling with specific antisera against the individual ns ...
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Human MxA Protein Confers Resistance to Semliki Forest Virus and Inhibits the Amplification of a Semliki Forest Virus-Based Replicon in the Absence of Viral Structural Proteins
... influenza virus and vesicular stomatitis virus (VSV) but also against Semliki Forest virus (SFV), a togavirus with a single-stranded RNA genome of positive ...
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Tryptic peptide analysis on nonstructural and structural precursor proteins from Semliki Forest virus mutant-infected cells.
... DISCUSSION The accumulation of large nonvirion proteins in cells infected with temperature-sensitive mutants of Semliki Forest virus allowed analysis of their tryptic peptides and compar[r] ...
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Chemical cross-linking of proteins of Semliki Forest virus: virus particles and plasma membranes from BHK-21 cells treated with colchicine or dibucaine.
... Chemical Cross-Linking of Proteins of Semliki Forest Virus: Virus Particles and Plasma Membranes from BHK-21 Cells Treated with Colchicine or Dibucaine CHRISTOPHER D.. VANCE* Department [r] ...
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Role of Conserved Histidine Residues in the Low-pH Dependence of the Semliki Forest Virus Fusion Protein
... trigger virus-membrane fusion. For alphaviruses such as Semliki Forest virus (SFV), acidic pH initiates a series of conformational changes in the heterodimeric virus envelope proteins ...
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Electron Microscopy of Cells Infected with Semliki Forest Virus Temperature-Sensitive Mutants: Correlation of Ultrastructural and Physiological Observations
... Electron Microscopy of Cells Infected with Semliki Forest Virus Temperature-Sensitive Mutants: Correlation of Ultrastructural and Physiological Observations Department of Microbiology, J[r] ...
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Semliki Forest Virus Budding: Assay, Mechanisms, and Cholesterol Requirement
... Our studies on virus budding also offer insights into cellular membrane budding processes, including the role of lipids in the budding reaction. Vesicular trafficking mediates the trans- port and sorting of cargo ...
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Effect of impaired glycosylation on the biosynthesis of Semliki forest virus glycoproteins.
... 3 and of SFV-infected cells labeled for 1.5 h with tritiated amino acids under various conditions of impaired glycosylation: A in Earle BSS supplemented with 5 mM glucose and 0.25 mM DG,[r] ...
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Control of protein synthesis in Semliki forest virus-infected cells.
... The viral structural proteins were detectable with certainty at 3.5 h postinfection, and their rate of synthesis increased linearly parallel to the amount of their messenger, the 26S RNA[r] ...
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Short-lived minus-strand polymerase for Semliki Forest virus.
... The untreated and RNase-treated portions were precipitated with trichloroacetic acid Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest ously reported a similar observatio[r] ...
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Semliki Forest virus multiplication in clones of Aedes albopictus cells.
... These studies showed that whereas events before negative-strand RNA synthesis occurred to the same extent in all clones examined, negative-strand RNA synthesis and all subsequent virus-s[r] ...
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Replication of Semliki Forest virus RNA in infected chick cells
... Estimation of the time required to synthesize a molecule of viral RNA As explained earlier Discussion, Section III 1, if RNA is being synthesized and labelled at a constant rate, the lab[r] ...
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Monensin inhibits Semliki Forest virus penetration into culture cells.
... block to virus entry imposed by both types of inhibitor can be circumvented readily by low pH-induced virus fusion at the plasma membrane (12), and substitution experiments indicate that[r] ...
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