Serotyping/methods
A REVIEW ON ADVANCED SEROTYPING METHODS FOR IDENTIFICATION OF KLEBSIELLA PNEUMONIAE CAPSULAR SEROTYPES
... wzc sequencing method was developed recently for wzc region. (36) The wzc gene (Fig. 1) functions to code for a protein responsible for capsule assembly. Seventy-six capsuler types have its wzc variable region sequenced ...
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Evaluation of Molecular Methods for Serotyping Shigella flexneri
... two methods but also showed that all of the phylogroups in their study contained multiple serotypes ...Phenotypic serotyping is expensive, labor-intensive, and diffi- cult to quality control and can result ...
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Methods for serotyping nasopharyngeal isolates of Haemophilus influenzae: slide agglutination, Quellung reaction, countercurrent immunoelectrophoresis, latex agglutination, and antiserum agar
... Methods for Serotyping Nasopharyngeal Isolates of Haemophilus influenzae: Slide Agglutination, Quellung Reaction, Countercurrent Immunoelectrophoresis, Latex Agglutination, and Antiserum[r] ...
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Comparison between methods for serotyping of Candida albicans produces discrepancies in results
... Abbreviations: A, serotype A; B, serotype B; auto, number of strains which autoagglutinated; S, strong agglutination reaction of yeast by H9 antibody; w, weak agglutination reaction with[r] ...
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International Quality Assurance Study for Characterization of Streptococcus pyogenes
... emm PCR ELISA and emm reverse line blot hybridization. The emm PCR ELISA was used in only one center (Table 2; London, United Kingdom) for the examination of strains that were negative with the classic serotyping ...
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Use of Phenotypic and Molecular Serotype Identification Methods To Characterize Previously Nonserotypeable Group B Streptococci
... Comparison of human and bovine isolates. A significant proportion of the initially nonserotypeable isolates from hu- mans were serotypeable when retested by improved methods (29, 31). However, these improved ...
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From Quellung to Multiplex PCR, and Back When Needed, in Pneumococcal Serotyping
... of serotyping for surveillance and vaccine efficacy ...for serotyping the invasive pneumococci isolated in Finland for serotype-specific surveillance purposes and has been applied in the serotyping ...
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Comparison of the Penner and Lior methods for serotyping Campylobacter spp
... RESULTS A comparison of the results of serotyping 1,405 Campylobacter isolates from human, animal, and environmental sources showed that 96.1% 1,350 and 92.1% 1,294 of strains were typab[r] ...
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Serotyping of Cryptococcus neoformans by dot enzyme assay
... for serotyping new isolates include the use of a combination of selective media and dif- ferential staining of yeast cells by indirect immunofluorescence ...molecular methods such as karyotyping (23), DNA ...
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Clinical Evaluation and Cost Analysis of Great Basin Shiga Toxin Direct Molecular Assay for Detection of Shiga Toxin Producing Escherichia coli in Diarrheal Stool Specimens
... performance (8, 9). Importantly, even with broth enrichment the sensitivity of EIA is low, ranging from 29 to 74% (7–9, 19). Moreover, the necessity of broth enrichment prior to EIA delays results for 24 to 36 h, which, ...
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Accuracy of High Throughput Nanofluidic PCR Based Pneumococcal Serotyping and Quantification Assays Using Sputum Samples for Diagnosing Vaccine Serotype Pneumococcal Pneumonia: Analyses by Composite Diagnostic Standards and Bayesian Latent Class Models
... reaction methods, especially because the quantity of the second most dominant serotype is, on average, 2 logs less than that of the most dominant serotype ...
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Evaluation of Methods for Subtyping Campylobacter jejuni during an Outbreak Involving a Food Handler
... The methods employed were somatic O sero- typing, PCR-restriction fragment length polymorphism (RFLP) analysis of flaA, DNA sequence analysis of 582 bp of flaA that included the short variable region (SVR), and ...
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Coagglutination test for serotyping Pasteurella haemolytica
... Several methods have been used for the serologic typing of ...for serotyping P. haemolytica (10). For the rapid serotyping of ...the methods presented above are time-con- suming and laborious ...
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Molecular genotyping of methicillin resistant Staphylococcus aureus via fluorophore enhanced repetitive sequence PCR
... bacteriological methods for epidemiologic analysis. Methods which have been used to monitor nosoco- mial MRSA outbreaks include phage typing (2), serotyping (10), protein electrophoresis (4), ...
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DETECTION AND SEROTYPING OF DENGUE VIRUS CIRCULATING IN FEBRILE HUMAN SUBJECTS IN JAZAN AREA, KINGDOM OF SAUDI ARABIA
... and serotyping circulating in human blood at Jazan area has limited information and the available diagnostic methods based on the detection of antibodies using ELISA ...
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Accuracy of Phenotypic Methods for Identification of Streptococcus pneumoniae Isolates Included in Surveillance Programs
... Of the methods used, bile solubility had the highest agree- ment with AccuProbe (98.8% sensitivity, 82.5% specificity). If the 11 bile solubility-negative isolates that were thought to be S. pseudopneumoniae are ...
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Phage Based Typing Scheme for Salmonella enterica Serovar Heidelberg, a Causative Agent of Food Poisonings in Canada
... typing methods such as serotyping, biotyping, phage typing, antimicrobial resistance testing, PFGE, and ribotyping to determine etiology and relatedness of outbreak ...
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Serotyping of Chicken salmonellae in Jos, Plateau state, Nigeria
... Serological characterization of chicken salmonellae isolated from four thousand, six hundred and sixteen (N= 4,616) samples from 1,319 chickens was done between 2006 and 2011 with the live bird market in Jos, Plateau ...
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Serotyping Streptococcus pneumoniae by Multiplex PCR
... correct serotyping of types and groups 1, 3, 4, 6, 14, 18, 19F, 19A, and ...for serotyping a sample by the capsular reaction with SSI antisera in our laboratory is ...
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Serotyping Cryptococcus neoformans by immunofluorescence
... Antisera obtained from all four rabbits immunized with the B551 antigen serotype A, when labeled, intensely stained cells of the homologous strain.. One conjugate, prepared from serum co[r] ...
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