SH2 Domains
Interactions of polyomavirus middle T with the SH2 domains of the pp85 subunit of phosphatidylinositol-3-kinase.
... Interaction between pp85 or its SH2 domains and middle T can be blocked by a synthetic peptide comprising the tyrosine phosphorylation sequence around middle T residue 315.. Despite the [r] ...
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Probing SH2-domains using Inhibitor Affinity Purification (IAP)
... mixed SH2-protein affinity ...many SH2 inhibitors and, therefore, may be considered as potential moiety for the probe ...The SH2 domain inhibitor 2 that was previously applied for a thermodynamic and ...
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Inhibition of binding of phospholipase CΓ1 SH2 domains to phosphorylated epidermal growth factor receptor by phosphorylated peptides
... The peptide was elongated by the sequential coupling of the following amino acids 2 mmol as their HOBT-activated esters in order of addition: N"-Boc-Gln, Nu-Boc-Gln, Nu-Boc-Pro, NaBoc-Il[r] ...
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Biological and biochemical activity of v-Crk chimeras containing the SH2/SH3 regions of phosphatidylinositol-specific phospholipase C-gamma and Src.
... Mutant viruses with Crk-SH2/SH2' domains induced tyrosine phosphorylation of cellular proteins, but mutants with phosphatidylinositol-specific phospholipase C--y or Src SH2/SH2' domains [r] ...
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Phosphotyrosine recognition domains: the typical, the atypical and the versatile
... reader domains, their interactions and the net- works that they serve can now be utilised and also war- rants further exploration into several ...reader domains should also be useful in a range of cli- ...
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Investigating Binding Partners of the Neuronal Adaptor Protein ShcC Involved in Learning and Memory
... and SH2 domains, to the SH3 and catalytic (SH1) domains of Src, respectively, and that binding of p66 Shc inhibited IGF-1- dependent Src kinase activation (Xi et ...
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Polyomavirus middle-T antigen associates with the kinase domain of Src-related tyrosine kinases.
... and SH2 domains are known to mediate interactions of Src-related kinases with a variety of proteins, it is conceivable that they are also required for association with ...of domains derived from ...
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Structural and biophysical investigation of the interaction of a mutant Grb2 SH2 domain (W121G) with its cognate phosphopeptide
... We used EPR spectroscopy in order to obtain structural information for the interaction of the wild-type Grb2 SH2 and the TrpEF1Gly Grb2 SH2 domains with the pYVNV peptide in solution. For the ...
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Structural and biochemical characterisation of pleckstrin homology domains
... invariant SH2 residue, an arginine which forms hydrogen bonds with two pTyr phosphate ...v-Crk SH2 domains have identified a conserved FLVRES sequence in this pocket, and demonstrated that the ...
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(Arg)9-SH2 superbinder: a novel promising anticancer therapy to melanoma by blocking phosphotyrosine signaling
... (SH2) domains specifically recognize phosphorylated tyrosine and mediate cell signal transduc- tion [9, ...” SH2 domain, with triple-point mutants Thr8Val/Cys10Ala/Lys15Leu, abbre- viated SH2 ...
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Diversity of Receptor Tyrosine Kinase Signaling Mechanisms
... In addition to the C-lobe/N-lobe interactions the intracellular juxtamembrane region (iJM) also plays an important role in EGFR activation. Among RTKs, JM regions differ significantly in their length, sequence, and ...
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Isolation and characterisation of α2-chimaerin cDNA encoding a p21rac GTPase-activating protein with an SH2 domain
... with SH2 domains from other proteins. The following SH2 domains were ...Src SH2- SH3 (a gift from T. Leung, IMCB) was used as it is the prototype SH2 domain; its involvement ...
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Temperature-Sensitive Transformation by an Abelson Virus Mutant Encoding an Altered SH2 Domain
... Although cells infected with P120/R273K displayed reduced levels of cellular phosphotyrosine at 39.5°C, typical rounded transformed cells could be found in the cultures. However, the frequency of these cells appeared to ...
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RNA virus modulation of IFN, PI3K and apoptosis
... two SH2 domains that promote interactions with signalling proteins and the catalytic subunit of ...p85 SH2 domains to phosphotyrosines (in a consensus YXXM motif) within activated receptors or ...
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Adapters in lymphocyte signaling
... cytoplasmic domains of the immunoreceptor-associated signal–transducing sub- units such as CD3 γ , - δ , and - ε , the TCR ζ -chains, and the BCR-associated signaling molecules Igα and Igβ (CD79a and ...tandem ...
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New conformational properties of SH2 domain binding pocket
... movement of SH2 domain binding pocket. Eight motifs and single amino acids are presented in SH2 domains binding pocket environment. They are forming pTyr-binding, central and hydrophobic part of ...
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SRC Homology 2 Domain Binding Sites in Insulin, IGF-1 and FGF receptor mediated signaling networks reveal an extensive potential interactome
... 38 SH2 families, 33 possess at least one gene duplicate allowing a duplicate copy to acquire new functions such as specialized tissue functions or novel scaffolding cap- abilities ...for SH2 domains ...
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The Carboxyl Terminus of v-Abl Protein Can Augment SH2 Domain Function
... v-Abl SH2 domain were replaced by those encoding the v-Src SH2 domain, pSR␣ viruses and pSAK (22) were ...the SH2 domains between pSAK and the pSR␣ viruses, the intermediate vector TA/P70 was ...
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C-Cbl negatively regulates TRAF6-mediated NF-κB activation by promoting K48-linked polyubiquitination of TRAF6
... proline-rich domains of c-Cbl are required for interactions with TRAF6 The major pathway for receptor ubiquitination requires interaction between the SH2 domain of c-Cbl and tyrosine phosphorylation of the ...
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PGRMC1 regulation by phosphorylation: potential new insights in controlling biological activity
... Progesterone receptor membrane component 1 (PGRMC1) is a multifunctional protein implicated in multiple pathologies, including cancer and Alzheimer’s disease. The recently published structure of PGRMC1 revealed ...
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