single-chain antibody
A TVA–Single-Chain Antibody Fusion Protein Mediates Specific Targeting of a Subgroup A Avian Leukosis Virus Vector to Cells Expressing a Tumor-Specific Form of Epidermal Growth Factor Receptor
... MR1 single-chain antibody. This antibody binds specifically to the extracellular region of EGFRvIII, a variant form of the EGFR that is expressed at the surface of human tumor cells, including ...
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Arg9 facilitates the translocation and downstream signal inhibition of an anti HER2 single chain antibody
... Currently, cell penetrating peptides (CPPs), including TAT and Arginine rich sequences, [4-6] have been iden- tified to have the capacity to help protein, peptide, PNA, siRNA or DNA to get across the biological membranes ...
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Llama Single-Chain Antibody That Blocks Lipopolysaccharide Binding and Signaling: Prospects for Therapeutic Applications
... The selected VHH 5G was functional after a denaturation- renaturation cycle. This property of correct refolding, which is intrinsic to VHHs in general, may prove to be highly favorable for the applications of the ...
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Llama-Derived Single-Chain Antibody Fragments Directed to Rotavirus VP6 Protein Possess Broad Neutralizing Activity In Vitro and Confer Protection against Diarrhea in Mice
... Llama-derived single-chain antibody fragments (VHH) are the smallest molecules with antigen-binding ca- pacity and can therefore be expected to have properties different from conventional ...
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Analysis of the relationship between end-to-end distance and activity of single-chain antibody against colorectal carcinoma
... bivalent single-chain antibodies targeting colorectal cancer with linkers of 5-15 amino acids ...bivalent single-chain antibody's affinity and ...
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Kinetic characterisation of a single chain antibody against the Hormone Abscisic Acid : comparison with its parental monoclonal
... A single-chain Fv fragment antibody (scFv) specific for the plant hormone abscisic acid (ABA) has been expressed in the bacterium Escherichia coli as a fusion ...monoclonal antibody and the ...
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Targeting melanoma with immunoliposomes coupled to anti-MAGE A1 TCR-like single-chain antibody
... Abstract: Therapy of melanoma using T-cells with genetically introduced T-cell receptors (TCRs) directed against a tumor-selective cancer testis antigen (CTA) NY-ESO1 demonstrated clear antitumor responses in patients ...
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Factors affecting the production of a single-chain antibody fragment (SCFV) by Aspergillus awamori in stirred tank reactors
... Another noticeable element from these two fermentation runs (F3 and F4) was the fact that the antibody levels reached their maximum level 4 0 - 50 hours after inoculation and then they either remained constant or ...
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Abrogation of Viral Interleukin-6 (vIL-6)-Induced Signaling by Intracellular Retention and Neutralization of vIL-6 with an Anti-vIL-6 Single-Chain Antibody Selected by Phage Display
... Cells and reagents. COS-7 and HepG2 cells were bought from the ATCC (Rockville, MD) and were routinely grown in Dulbecco’s modified Eagle’s me- dium (DMEM) supplemented with 10% fetal calf serum (FCS). The vIL-6 DNA ...
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Transient and Stable Expression of Single-chain Antibody Fragment scFv13R4 in Chinese Hamster Ovary Cells.
... are in agreement, the concentration values are averaged, giving elution 1 a concentration of 134.635 µg/mL and elution 2 a concentration of 102.603 µg/mL. Averaging the concentration across the two elutions where the ...
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Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery
... one single-chain bispecific diabody (scDb, containing the two scFvs together; Figure 3B) and evaluated their capacity in delivering liposome-encapsulated doxorubicin to the target cells ...bispecific ...
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Single-Chain Antibody Fragment Specific for Plasmodium vivax Duffy Binding Protein
... cytes by other Plasmodium species, such as P. falciparum. The erythrocyte-binding capabilities of several proteins have been examined, including merozoite surface protein 1, reticulocyte binding protein, and DBP, which ...
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Effects of different interchain linkers on biological activity of an anti-prostate cancer single-chain bispecific antibody
... bivalent single-chain MABL sc(Fv)2 with the linker (Gly4Ser)3 of 15 amino acids and showed that the recombinant antibody and parental antibody had almost the same affinity for the antigen and ...
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Targeted tumor killing via an intracellular antibody against erbB 2
... intracellular antibody directed against the erbB-2 ...anti-erbB-2 single chain antibody to achieve targeted tumor cell killing in vivo and can show significantly prolonged survival of animals ...
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Paracrine Pax6 activity regulates oligodendrocyte precursor cell migration in the chick embryonic neural tube
... anti-Pax6 single-chain antibody, which is significant, as indicated by the quantifications shown in ...proper antibody folding was further demonstrated by the absence of activity of the SaP6- ...
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Recombinant Single-Chain Variable Fragment Antibodies Directed against Clostridium difficile Toxin B Produced by Use of an Optimized Phage Display System
... our single-chain antibodies as the capture antibody in the ELISA procedure, although under these conditions the small scFv molecule can undergo partial denaturation ...of single-chain ...
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Improvement of retroviral retargeting by using amino acid spacers between an additional binding domain and the N terminus of Moloney murine leukemia virus SU.
... of single-chain antibody fragments (14) or of ligands for two different cell surface molecules (3): the epidermal growth factor receptor (EGFR) and the Ram-1 phosphate transporter, a cell surface ...
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Development of a novel small antibody that retains specificity for tumor targeting
... parental antibody-colicin Ia fusion protein (Fab-Ia), single-chain antibody-colicin Ia fusion protein (Sc-Ia) (CL(Xi'an) Bio-scientific) and nonrelative control protein, low molecular weight ...
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zjv9113.pdf
... for single-chain-antibody-fragment (scAb) expression, a C-terminal 6-histidine tag for purification, and the Skp chaperone to aid soluble-protein expression in the periplasmic space ...
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Expression of a Functional Single-Chain Variable-Fragment Antibody against Complement Receptor 1 in Streptococcus gordonii
... the antibody to bind to the Fc ␥ III/II receptors to eliminate background binding in subsequent ...(1/200) antibody was added to the first three aliquots, and PBS-BSA buffer was added to the fourth ...
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