Single-chain variable fragment antibodies (scFv)
Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C
... recombinant antibodies was low- productive for scFv ...of scFv 9E was inserted into the pET-22b vector by the NcoI/NotI sites ...for scFv 9E molecular weight (about 30 kDа) were found ...the ...
7
Recombinant Single-Chain Variable Fragment Antibodies Directed against Clostridium difficile Toxin B Produced by Use of an Optimized Phage Display System
... enough scFv product, five assembly SOE-PCR runs were performed as above, pooling the PCR products, with ethanol precipitation and washing as usual (34, ...gel-purified scFv fragment was digested with ...
9
Single-Chain Antibody Fragment Specific for Plasmodium vivax Duffy Binding Protein
... display antibodies offer a way to produce high-affinity single-chain variable fragment (scFv) derivatives of human an- tibodies of “natural host” origin ...monoclonal ...
6
Cloning approach and functional analysis of anti intimin single chain variable fragment (scFv)
... anti-intimin scFv The amplified fragments corresponding to VL and VH were cloned into pGEM-T Easy vector and sequenced using the M13 primers (forward and ...the variable region of antibodies of the ...
9
Isolation of a Novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library
... Human antibodies are more preferred than murine and chimeric antibodies because they induce low immunogenicity in their ...human antibodies is the phage display technology (PDT), which was first ...
11
Synthesis of a soluble flag tagged single chain variable fragment (scFv) antibody targeting Cucumber Mosaic Virus (CMV) coat protein
... L chain DNA fragments by restriction endonucleases, and the additional tag sequence at the carboxyl terminus of the scFv was added to facilitate the detection and purification in subsequent steps (Clackson ...
8
Expression of a Functional Single-Chain Variable-Fragment Antibody against Complement Receptor 1 in Streptococcus gordonii
... HA-specific antibodies in sera and saliva, microtiter plates were coated with 100 ng/well recombinant cyclophilin 18 (rC18) in ELISA coat- ing buffer at 4°C ...PCR fragment encoding the mature C18 was ...
7
Recombinant production of the antibody fragment D1.3 scFv with different Bacillus strains
... antibody fragment with high-affinity binding to an antigen is the single-chain fragment variable (scFv) [27], a heterodimer comprising the antibody heavy- and light-chain ...
18
<p>Efficacy of a recombinant single-chain fragment variable region, VasSF, as a new drug for vasculitis</p>
... We wanted to determine whether VAP2 is a VasSF anti- gen that binds VAP2 in human plasma in vitro. Therefore, anti-VAP2 polyclonal antibodies (aVAP2) were injected into SCG/Kj mice to alleviate their vasculitis ...
14
Combination of specific single chain antibody variable fragment and siRNA has a synergistic inhibitory effect on the propagation of avian influenza virus H5N1 in chicken cells
... antibodies [20]. However, Y2H library screening also generates a significant number of false-positive interac- tions, including “biological” false-positives: protein–pro- tein interactions that occur in yeast ...
8
Extracellular vesicles expressing a single-chain variable fragment of an HIV-1 specific antibody selectively target Env+ tissues
... For western blot analysis, ultracentrifuged exosomal pellets were lysed with RIPA buffer (Cell Signaling Technology) containing a Protease Inhibitor Cocktail (Calbiochem). The total protein was determined using a BCA ...
15
Efficient production of single-chain fragment variable-based N-terminal trimerbodies in Pichia pastoris
... recombinant antibodies in bioanalytical and medical applications ...of antibodies, new recombinant for- mats with modified properties have been generated ...multispecific antibodies capable of sim- ...
9
Construction, expression and binding specificity of bispecific CD3 × VEGFR 2 and CD3 × NCAM antibodies in the single chain and diabody format
... monoclonal antibodies specifically recog- nize a single antigen with their N-terminal variable do- ...produced antibodies such as immune therapies it is of interest to avoid potential excess ...
11
Current applications of antibody microarrays
... the single-color approach, labels can be introduced at different efficacy in different samples due to chemis- try dye binding stronger to certain amino ...a single fluo- rescent reporter dye, ...
15
The YoaW signal peptide directs efficient secretion of different heterologous proteins fused to a StrepII-SUMO tag in Bacillus subtilis
... GFP-specific scFv is marked with two stars (**), and StrepII-SUMO is marked with three stars ...GFP-specific scFv antibody fragments were bound to magnetic beads via the C-terminal polyhistidine tag (lanes ...
14
zjv9113.pdf
... highest-affinity scFv genes isolated from the off-rate analysis described above, along with the 80R scFv gene, were chosen for DNA ...80R scFv and then digested with ...
9
Surface display of proteins by Gram-negative bacterial autotransporters
... a single polypeptide that contained all the information necessary for secretion ...are single polypeptides that contain two domains, one which is secreted across the outer mem- brane and another one which ...
15
Platelet-Targeted Delivery of Peripheral Blood Mononuclear Cells to the Ischemic Heart Restores Cardiac Function after Ischemia-Reperfusion Injury
... These findings strongly imply that transplantation of platelet-targeted-PBMCs might be an effective and novel therapeutic approach for AMI. Intravenous cell delivery is the least invasive and most convenient route, and ...
15
Gene therapy for carcinoma of the breast: Genetic ablation strategies
... such as phospholipase C-γ, phosphatidylinositol 3-kinase, guanosine triphosphatase-activating protein, and the adapter protein SHC [22]. Gene therapeutic modulation of the basal signal transduction apparatus could ...
5
The expression and metal affinity purification of single-chain antibodies
... Colcher et al. (1981) immunized mice with a membrane-enriched fraction of human metastatic mammary carcinoma cells found in liver. Spleen cells from the mice were fused with NS-1 myeloma cells and supernatant fluids from ...
265