Single-domain antibodies
High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli
... [8-11]. Single- domain antibodies (sdAbs), which are derived from camelid heavy chain-only antibodies and which consist solely of the antigen-specific domain [12], offer multiple ...
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Intracellular Expression of Camelid Single-Domain Antibodies Specific for Influenza Virus Nucleoprotein Uncovers Distinct Features of Its Nuclear Localization
... of single-domain antibodies that target influenza virus NP in the cytosol of influenza virus- infected cells and studied their impact on influenza virus replica- ...
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Single-Domain Antibodies Targeting Neuraminidase Protect against an H5N1 Influenza Virus Challenge
... specific single-domain antibodies (N1-VHHm) and evaluated their in vitro and in vivo antiviral ...Moreover, single-dose prophylactic treatment with bivalent N1-VHHb or N1-VHH-Fc protected ...
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Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies
... llama single domain antibodies (VHHs) fused to IgA, produced in Arabidopsis thaliana seeds and fed to piglets resulted in a progressive decline in shedding of F4 positive ETEC ...on ...
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Straightforward Selection of Broadly Neutralizing Single-Domain Antibodies Targeting the Conserved CD4 and Coreceptor Binding Sites of HIV-1 gp120
... Using a nonspecific elution to recover the phage bound to the selection antigen, most of the best sdAb candidates isolated were dominant clones from the output of basic phage display-based in vitro selection. ...
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Selection, characterization, and thermal stabilization of llama single domain antibodies towards Ebola virus glycoprotein
... The cause of the extreme virulence by Ebola virus infection is still not well established. However current data suggests that the interaction of glycosylated enve- lope protein (glycoprotein) with the immune system plays ...
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Identification and functional characterization of unique single domain antibodies against pro-apoptotic protein Bax.
... Despite the various causes o f MOMP, in all cases this event most commonly results in release o f the IMS protein cytochrome c (normally functioning as vital component o f the electron transport chain) and ultimate cell ...
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Isolation of anti-extra-cellular vesicle single-domain antibodies by direct panning on vesicle-enriched fractions
... ventional antibodies—generally poly- and monoclonal antibodies of the IgG class—have been successfully used for immune-affinity purification of EV sub-groups ...26]. Single-domain antibod- ies ...
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Isolation and characterization of camelid single domain antibodies against HER2
... © The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creat iveco mmons .org/licen ses/by/4.0/), which permits unrestricted use, ...
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High-Resolution Epitope Positioning of a Large Collection of Neutralizing and Nonneutralizing Single-Domain Antibodies on the Enzymatic and Binding Subunits of Ricin Toxin
... panel; see also Fig. S1 in the supplemental material). In terms of RTB, we identified an aggregate of neutralizing epitopes localized at the RTA-RTB interface positioned in close proximity to the Gal/GalNAc recognition ...
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Biotechnological applications of recombinant single-domain antibody fragments
... gle-domain antibodies and the possibility to produce them as recombinant proteins fused to suitable tags make them inexpensive binders that can be easily oriented at high density on capture surfaces such as ...
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Single domain antibody fragments derived from heavy chain antibodies: a review
... heavy-chain antibodies in camelids and sharks (Conrath et ...“Single Domain Antibodies” (Saerens and Muyldermans 2012) has been launched with a complete methodology and key protocols for the ...
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IFN λ1 with Th17 axis cytokines and IFN α define different subsets in systemic lupus erythematosus (SLE)
... Systemic lupus erythematosus (SLE) is a heterogeneous autoimmune disease. One hallmark of SLE is the pres- ence of autoantibodies against nuclear constituents (antinuclear antibodies [ANA]), including anti-double- ...
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Natural and Induced Magnetization in the Bobolink, Dolichonyx Oryzivorus (Aves: Icteridae)
... Because of the manner in which single domain SD and pseudo-single domain PSD magnetite particles attempt to align themselves with the ambient magnetic field, it seems plausible that magn[r] ...
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Dissociable psychosocial profiles of adolescent substance users
... although single-item questions have ethical and practical advantages over more comprehensive measures [52], it is likely that single-item questions used in the current study ...
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Samba3 HOWTO pdf
... The BDC is said to hold a read-only of the SAM from which it is able to process network logon requests and authenticate users. The BDC can continue to provide this service, particularly while, for example, the wide- area ...
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Single-Carrier Frequency-Domain Equalizer
... This paper analyzes the single-carrier frequency domain equalizer (SC-FDE) for two common transmit diversity schemes: cyclic delay diversity (CDD) and Alamouti signalling. We characterize the diversity for ...
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Conformational changes affecting the V3 and CD4-binding domains of human immunodeficiency virus type 1 gp120 associated with env processing and with binding of ligands to these sites.
... Monoclonal antibodies MAbs that compete for binding of gpl20 to CD4 have been previously described; these include antibodies directed against linear epitopes located within the C4 domain[r] ...
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The Antiviral Mechanism of an Influenza A Virus Nucleoprotein Specific Single Domain Antibody Fragment
... FIG 3 ␣ NP-VHH1 binds to the body domain of NP. (A) NP alone (blue) or preincubated with an excess of ␣ NP-VHH1 (red) was subjected to size exclusion chromatography with a Superdex 200 column. Absorbance at 280 nm ...
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Evaluation of envelope domain III based single chimeric tetravalent antigen and monovalent antigen mixtures for the detection of anti dengue antibodies in human sera
... In both IgM and IgG indirect ELISAs the sensitivities manifested a small but significant improvement when the coating antigen was the single tetravalent antigen com- pared to the physical mixture of four EDIIIs ...
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