single-molecule force spectroscopy (SMFS)
Efficient unfolding pattern recognition in single molecule force spectroscopy data
... the single-molecule force spectroscopy (SMFS) method was established for experimental investigations on pro- teins (membrane and globular) and cells ...characteristic Force- ...
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Single-molecule force spectroscopy on histone h4 tail cross-linked chromatin reveals fiber folding
... more gradual unstacking transition for 167-bp NRL MT fibers under reducing conditions (Fig. 4A). Likewise, sedimentation velocity analysis of 167- and 177-bp NRL MT fibers under reducing conditions indicated a reduced ...
9
Verdorfer, Tobias (2018): Multiplexed single-molecule force spectroscopy and activity studies on cellulosomes. Dissertation, LMU München: Fakultät für Physik
... unfolding force distributions of Coh1 and T107S indicates that both cohesins were stabilized by dockerin ...a single fold through binding of the dockerin, resulting in an unfolding behaviour dominated by a ...
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Ott, Wolfgang Bernhard (2017): Single molecule force spectroscopy with biological tools. Dissertation, LMU München: Fakultät für Physik
... speed force spectroscopy AFM based on an Ando-type high speed imaging AFM (Ando et ...traditional SMFS framework, but can be improved by custom cantilever optimiza- tion procedures at the cost of ...
305
Opfer, Jan (2012): Single-molecule force spectroscopy studies of integrin-mediated cell signaling. Dissertation, LMU München: Fakultät für Physik
... On the here evaluated molecular level, no significant increase in the adhesion rate caused by SDF- 1α was observed. Since the interaction is not ligand-limited, this argues that SDF-1α does not prominently activate ...
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Life in extreme environments: Single molecule force spectroscopy as a tool to explore proteins from extremophilic organisms
... of single protein ...examining single molecules one at a time, the individual dynamics of subpopulations can be ...perturb single protein molecules using ...
14
Klamecka, Kamila (2017): Single-molecule force spectroscopy of biological complexes. Dissertation, LMU München: Fakultät für Biologie
... obtained force distribution. The primary peak was attributed to the single-molecule events, and the following ones to multiple ...rupture force of the complex was characterized by AFM ...
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Application of Fuzzy Logic Algorithm to Single Molecule Force Spectroscopy of the Streptavidin Biotin System
... a single ligand-receptor complex, we need to col- lect the force curves with the single-molecule event ...1000 force-separation ...interaction force from each ...
10
Nanomechanical and thermophoretic analyses of the nucleotide-dependent interactions between the AAA+ subunits of magnesium chelatase
... retained. Single molecule force spectroscopy (SMFS) was used to determine the factors that stabilize formation of the ChlID − MgADP complex at the single molecule level; ...
7
Extraction of accurate biomolecular parameters from single-molecule force spectroscopy experiments
... from single-molecule forced-dissociation experiments is presented that considers attachment position on the AFM tip, geometric effects, and polymer dynamics of the ...
10
Single-molecule force spectroscopy studies of fibrin A-a polymerization interactions via the atomic force microscope
... fibrinogen molecule to lengthen may be the cause of the impaired polymerization of fibrinogen variants with reduced calcium binding at the γ 1 ...a single event indicated that the ‘A–a’ bond rarely ruptured ...
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A peptide‐display protein scaffold to facilitate single molecule force studies of aggregation‐prone peptides
... used single molecule force spectroscopy (SMFS) to probe the first dimerisation events of the central aggregation-prone region of Syn (residues 71-82) that may initiate ...
50
Jobst, Markus A. (2018): Multiplexed single molecule observation and manipulation of engineered biomolecules. Dissertation, LMU München: Fakultät für Physik
... of single amino acids on specific functions or stability. In force spectroscopy assays, addition and exchange of binding tags and mutations to the domains of interest are frequently performed to ...
245
Single-Molecule Unbinding Forces between the Polysaccharide Hyaluronan and Its Binding Proteins
... developed single-molecule force spectroscopy platform to analyze and compare the mechanical strength of bonds between HA and a panel of hyaladherins from the Link module superfamily, namely ...
13
Toehold-enchanced lna probes for selective pull down and single-molecule analysis of native chromatin
... impedes single molecule force spectroscopy experiments, which have been instrumental to probe chromatin ...subsequent single molecule analysis by magnetic ...of force. We ...
9
Force detected, single molecule spectroscopy and imaging using nanoscale mechanical resonators
... absorption spectroscopy of a single pentacene molecule embedded in a p-terphenyl host crystal cooled to ...work, single-molecule spectroscopy has exploded into a field of intense ...
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Baumann, Fabian (2016): Atomic force microscopy for high-specificity manipulations of proteins and high-throughput analysis in nanoapertures. Dissertation, LMU München: Fakultät für Physik
... effector molecule, conformational changes take place in the regulated protein allowing for (or inhibiting) the binding of the processed ...effector molecule in allosteric ...of force has been ...
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Imaging and kinetics of the bimolecular complex formed by the tumor suppressor p53 with ubiquitin ligase COP1 as studied by atomic force microscopy and surface plasmon resonance
... the single-molecule level, by using atomic force microscopy (AFM) and atomic force spectroscopy (AFS) and also in bulk solution by using surface plasmon resonance ...the ...
9
Observation of Single-Molecule Rotational Diffusion at Microsecond Timescale by Polarized Fluorescence Correlation Spectroscopy
... electronic force microscopy measurements of individual quantum dots, which have indicated that under illumination the quantum dot core frequently displays a transient elementary positive ...
357
Folding and Assembly of Cytoskeletal Proteins Under Force - From Single Molecule Studies of Dystrophin to Studies of Intact Cells
... a single AON designed to skip exon 51 (van Deutekom 2007) lead to modest levels of protein (<35% of normal controls) using standard antibody approaches that remain state of the art in expression analysis ...
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