Single strand break repair
SUMO modification of the neuroprotective protein TDP1 facilitates chromosomal single-strand break repair
... TDP1 is a substrate for modiication by SUMO. Yeast two-hybrid analyses conducted to identify novel TDP1 binding partners uncovered 14 independent clones encoding full-length UBE2I, the human homologue of the yeast ...
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Functional studies of accessory factors associated with base excision repair
... excision repair (BER) and single strand break repair (SSBR) (Caldecott, 2003a; Caldecott, ...pathways repair damage to DNA bases, from oxidation or covalent binding of non- bulky ...
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Meiotic Double-Strand Break Proteins Influence Repair Pathway Utilization
... We reasoned that the presence of intact univalents in rad-51; him-5 could be explained in two ways: either RAD-51 con- tributes to DSB formation (either directly or via positive feed- back); or, HIM-5 prevents the use of ...
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The Effect of Gap Length on Double-Strand Break Repair in Drosophila
... DSB repair are often classified according to whether or not a homologous template, usually the sister chromatid or the homolog, is ...or single-strand annealing (F ishman -L obell et ...templated ...
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Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in Leishmania
... No single current gRNA design program tested can accurately predict gRNA activity in Leishmania, and the gene-targeting efficiency for the same gRNA construct can vary greatly in different Leishmania ...a ...
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Analysis of Repair Mechanisms following an Induced Double Strand Break Uncovers Recessive Deleterious Alleles in the Candida albicans Diploid Genome
... rare repair events associated with DNA ...a single locus for DNA DSB induction on Chr4 and our results could be locus specific; additional experiments should be conducted to extend our study to other ...
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Novel treatment strategies in triple-negative breast cancer: specific role of poly(adenosine diphosphate-ribose) polymerase inhibition
... as single agents, in tumors harboring defects in double-strand DNA-break repair, the best studied being ovarian and breast cancers with BRCA germ-line mutations, in which they have induced ...
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Incorporation of Large Heterologies Into Heteroduplex DNA During Double-Strand-Break Repair in Mouse Cells
... a single copy of the vector into the chromosomal -locus generates a tan- dem duplication of the shared C-region of ...independent, single-copy targeted recombinants (Table ...
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Double-Strand Break Repair by Non-homologous End-Joining
... The single-stranded regions are then bound by RAD51 and this protein catalyses invasion and pairing of the ss tail with a sister chromatid (Sung, 1994; Baumann ...by strand invasion includes branch ...
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Differential Effects of DNA Double-Strand Break Repair Pathways on Single-Strand and Self-Complementary Adeno-Associated Virus Vector Genomes
... complementary strand synthesis, they can be used to specifically test the con- tributions of DNA repair factors to recombination indepen- dent of dsDNA ...DNA repair-deficient cell lines and found ...
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Differential Usage of Alternative Pathways of Double-Strand Break Repair in Drosophila
... double-strand break at a specific location such that expression of the red fluorescent protein, DsRed, in the next generation can be used to infer the frequency at which each pathway was ...conversion ...
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Coordination of DNA Ends During Double-Strand-Break Repair in Bacteriophage T4
... dependent strand annealing (SDSA) model (Nassif et Both the Szostak et ...a single zer 1996; George et al. 2001). Evidence for the Szostak repair event on the same homologous repair ...
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Mechanisms of Double-Strand-Break Repair During Gene Targeting in Mammalian Cells
... Ng, P., and M. D. Baker, 1998 High efficiency, site-specific modifi- repair mechanism. Proc. Natl. Acad. Sci. USA 87: 4680–4684. cation of the chromosomal immunoglobulin locus by gene tar- Sun, H., D. Treco and J. ...
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Possible loss of length conservation and reciprocity during recombination or conversion in tandem arrays.
... This loss of length conservation will occur in certain conditions in single-strand nick mechanisms of recombination, and in many situations in double-strand break repair recombina[r] ...
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Genetic Requirements for the Single-Strand Annealing Pathway of Double-Strand Break Repair in Saccharomyces cerevisiae
... A densitometric analysis of these data showed that in the wild-type strain (Figure 5a) the whole recombina- tion product (deletion plus gene conversion) repre- sented alm[r] ...
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MTE1 Functions with MPH1 in Double-Strand Break Repair
... Input and IP samples from each experiment were sequenced on an Illumina HiSeq 2500 (50 nucleotide single-end reads). All sequencing data are deposited in the Sequence Read Archive (http://www.ncbi.nlm.nih.gov/sra, ...
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Double-Strand Break Repair in Tandem Repeats During Bacteriophage T4 Infection
... Recombinational repair of double-strand breaks in tandemly repeated sequences often results in the loss of one or more copies of the ...The single-strand annealing (SSA) model for ...
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The role of homologous recombination in mitotic and meiotic double-strand break repair
... been identified: BLM, WRN, RecQL, RecQ4 and RecQ5. Mutations in BLM, WRN and RECQ4 cause Bloom’s syndrome, Werner’s syndrome and Rothmund- Thompson syndrome, respectively. Hallmarks of these three autosomal recessive ...
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Evidence for conservative (two-progeny) DNA double-strand break repair.
... Intramolecular double-strand break repair in the RecE pathway (in a recBC sbcA strain): As our aim is in demonstration of intramolecular recombination and, therefore, cons[r] ...
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Characterisation of unusual DNA glycosylases from Trichomonas vaginalis
... differences between isolates. Earlier work on genetic diversity of T. vaginalis involved isoenzyme analysis and antigen characterisation (Soliman et al., 1982; Krieger et al., 1985). Later studies utilised large samples ...
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