Single-strand DNA binding protein
Identification of a Domain of the Baculovirus Autographa californica Multiple Nucleopolyhedrovirus Single-Strand DNA-Binding Protein LEF-3 Essential for Viral DNA Replication
... for DNA replication in tran- sient assays, the ability of the lef-3 knockout and rescue bac- mids to replicate viral DNA in insect cells was ...intracellular DNA was harvested, and the amount of ...
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Identification of a Region of the Herpes Simplex Virus Single-Stranded DNA-Binding Protein Involved in Cooperative Binding
... major single-strand- DNA-binding protein (SSB) present in infected ...viral DNA (7, ...a DNA-negative phenotype as well as abnormalities in the production of viral ...
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Characterization of the DNA-Binding Domain of the Avian Y-Box Protein, chkYB-2, and Mutational Analysis of Its Single-Strand Binding Motif in the Rous Sarcoma Virus Enhancer
... two DNA-protein ...to DNA. An examination of the sequence of the noncoding strand of the RSV LTR, immediately to the 39 side of the octamer motif 59-GTACCACC-39 (2112 to 2119), revealed the ...
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A Single-stranded DNA-binding Protein of Bacteriophage T7 Defective in DNA Annealing
... to binding ssDNA and physically interacting with T7 DNA polymerase, gene ...2.5 protein also facilitates the an- nealing of complementary strands of DNA (10, 11, ...ogous DNA annealing ...
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The 60-Residue C-Terminal Region of the Single-Stranded DNA Binding Protein of Herpes Simplex Virus Type 1 Is Required for Cooperative DNA Binding
... a binding pattern characteristic of multiple independent sites, in striking contrast with the highly cooper- ative behavior of the full-length ICP8 and ...the binding of the C-terminally truncated proteins ...
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Single-strand DNA Binding by the Helix-Hairpin-Helix Domain of XPF Contributes to Substrate Specificity of ERCC1-XPF
... to DNA provide detailed insights into incision by XPF in archaea bacteria ...previous DNA-binding studies revealed that the HhH domains of ERCC1-XPF show a similar substrate specificity as the native ...
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Replication Protein A Is Required for Meiotic Recombination in Saccharomyces cerevisiae
... transient DNA double-stranded breaks ...3⬘ single-stranded DNA ends that serve to channel DSBs into the RAD52 recombinational repair ...replication protein A (RPA)—play a role in the ...
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The role of telomeres and telomerase in hematologic malignancies and hematopoietic stem cell transplantation
... ss DNA: Single-strand DNA; ds DNA: Double-strand DNA; TRF1: Telomeric repeat-binding factors 1; TRF2: Telomeric repeat-binding factors 2; TIN2: ...
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Helicase Motif Ia Is Involved in Single-Strand DNA-Binding and Helicase Activities of the Herpes Simplex Virus Type 1 Origin-Binding Protein, UL9
... Spodoptera frugiperda (Sf21) insect cells were maintained in Grace’s medium supplemented with 10% (vol/vol) fetal calf serum, 0.1 mg of streptomycin per ml, and 100 U of penicillin per ml. Escherichia coli DH5␣ cells ...
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A sequence-specific single-strand-binding protein for the late-coding strand of the simian virus 40 control region.
... Isolation of Z-DNA binding Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest DISCUSSION We have purified a single-strand-binding protein that binds in vitro to specific r[r] ...
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DNA DSB repair dynamics following irradiation with laser-driven protons at ultra-high dose rates
... set up and Beam characterization. Cells were irradiated at the Rutherford Appleton Laboratory, Science and Technology Facilities Council, United Kingdom using the Gemini laser system which is able to deliver ~12 J in a ...
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Human Papillomavirus DNA Replication Compartments in a Transient DNA Replication System
... E2 protein and a requirement of L2 for the localization of E2 in ...of protein expressed from the two ...E2 protein from a recombinant Semliki Forest virus in cells which harbor endogenous BVP-1 ...
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Studies on DNA Topology and DNA-Amyloid Beta (Aβ) peptides interaction in relevance to Alzheimer's Disease
... precursor protein sequence) is now considered as a unifying pathological feature of diverse forms of ...modulating DNA topology and possibly contribute to the BZ-DNA helical transition associated ...
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Large T-antigen mutants define multiple steps in the initiation of simian virus 40 DNA replication.
... In the presence of RF-A, a eucaryotic single-stranded DNA-binding protein required for origin-specific unwinding and the initiation of DNA replication 23, 81, and a topoisomerase which r[r] ...
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Evaluation of DNA Integrity of Cryopreserved Boer Goat (Capra hircus) Sperm Using Comet Assay at Various pH Conditions
... double-strand DNA breaks (neutral comet assay) were significantly higher than single-strand DNA breaks (alkaline comet ...double-strand DNA breaks compare to the ...
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Dominant mutations in mtDNA maintenance gene SSBP1 cause optic atrophy and foveopathy
... the sequence shift we experimentally found. However, the new sequence assign- ment revision did not improve the crystallographic phases, and the electron density was still ambiguous at the C-terminus (14). Our struc- ...
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Electron microscopic mapping of RNA transcribed from the late region of polyoma virus DNA.
... In the gene 32 protein spreads, several hybrid molecules with no single-strand loop larger than 100 nucleotides, with a duplex region longer than that corresponding to the RNA-DNA duplex[r] ...
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Phylogenetic and Functional Analysis of the Bacteriophage P1 Single-Stranded DNA-Binding Protein
... a single-stranded DNA-binding protein (SSB-P1), which shows 66% amino acid sequence identity to the SSB protein of the host bacterium Escherichia ...
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An alternative novel tool for DNA editing without target sequence limitation: the structure guided nuclease
... genomic DNA templates were prepared from five 6 hpf embryos randomly selected by incubating the embryos with 10-μL B solution under a program (65 °C for 30 min, 95 °C for 10 min, and 16 °C for 1 min) following the ...
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MOLECULAR MODELLING OF SINGLE WALLED CARBON NANOTUBE WITH SINGLE STRAND DNA
... a single strand DNA were also ...of single-wall zigzag, armchair, and chiral carbon nanotubes of varying length and diameter with single strand DNA were ...
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