single-stranded-DNA
Displacement of the canonical single stranded DNA binding protein in the Thermoproteales
... Single-stranded DNA binding proteins (SSBs) based on the OB-fold are considered ubiquitous in nature and play a central role in many DNA transactions including replication, recombination and ...
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Nucleoside Analogue Mutagenesis of a Single-Stranded DNA Virus: Evolution and Resistance
... threshold. Single-stranded DNA viruses are the fastest-mutating DNA-based systems, with per-nucleotide mutation rates close to those of some RNA viruses, but chemical mutagenesis has been much ...
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Analyses of the Interaction between the Origin Binding Domain from Simian Virus 40 T Antigen and Single-Stranded DNA Provide Insights into DNA Unwinding and Initiation of DNA Replication
... for DNA replication; the references to the individual mutations are ...double-stranded DNA substrates ...to single-stranded DNA, oligomerization on origin-containing DNA, ...
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Binding of the pseudorabies virus immediate-early protein to single-stranded DNA.
... Results of early studies have shown that ICP4 from infected cell extracts binds to single-stranded DNA-cellulose columns 6.. Wilcox et al 17 have reported that ICP4 exists in several pho[r] ...
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Electrochemical Studies on the Recognition of a Ternary Copper Complex to Single-Stranded DNA and Double-Stranded DNA
... sperm DNA from Beijing Baitai Biochemistry Technology Company (Beijing, China) was used as ...Denatured single-stranded DNA (ssDNA) was produced by thermal denaturation [21]: ...
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Reovirus Protein ςNS Binds in Multiple Copies to Single-Stranded RNA and Shares Properties with Single-Stranded DNA Binding Proteins
... bind single-stranded RNA (ssRNA) molecules in gel mobility shift ...to single molecules of ssRNA with positive coop- erativity and in numbers dependent on RNA length such that each unit of NS ...
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Widely Conserved Recombination Patterns among Single-Stranded DNA Viruses
... The combinatorial nature of genetic recombination can potentially provide organisms with immediate access to many more positions in sequence space than can be reached by mutation alone. Recombination features ...
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Transfection of REP- mycoplasmas with viral single-stranded DNA.
... To determine if this increase in infectious centers was due to productive L51 infection of REP- cells, the experiment was repeated cells processed for transfection before addition of vir[r] ...
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Regions of incompatibility in single-stranded DNA bacteriophages phi X174 and G4.
... Effect of the G4 reduction sequence on the transduction of single-stranded plasmid particles Plasmid DNA Plasmid DNA in transducing particle Resident plasmid in host cell for the followi[r] ...
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A Single-stranded DNA-binding Protein of Bacteriophage T7 Defective in DNA Annealing
... If indeed the lethality is due solely to the defect in mediating homologous base pairing, then it would suggest that the an- nealing activity is essential for T7 survival. DNA annealing is important in both ...
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Robust Genome Editing with Short Single-Stranded and Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans
... on DNA- driven Cas9 activity, and, because coinjected DNA vectors are generally inherited together, Mello et ...coinjected DNA re- pair templates (Kim et ...large DNA templates required for ...
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In Vivo Conversion of the Single-Stranded DNA of the Kilham Rat Virus to a Double-Stranded Form
... Velocity sedimentation in a 10 to 30%7O neutral sucrose gradient of Hirt supernatant fluid taken 60 min after infection from a rat nephroma cell monolayer infected with KRV containing 3H[r] ...
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In vitro synthesis of double-stranded DNA from the Kilham rat virus single-stranded DNA genome.
... SALZMAN,* PHYLLIS FABISCH, RONALD PARR, CLAUDE GARON, AND TONU WALI Laboratory of Biology of Viruses, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014 Doub[r] ...
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Graphitic Surface Attachment by Single-Stranded DNA and Metal Nanoparticles
... 7.1 – work function and melting surface tension of selected metals 7.2 – SEM image of gold nanoparticles on 2, 3, and 13 layer graphene 7.3 – SEM imges of gold nanoparticles on 1, 2, [r] ...
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Double-Length, Circular, Single-Stranded DNA from Filamentous Phage
... In the CsCl gradient fractions, the length distribution of the DNAs was determined from measurements of at least 100 circular monomers and 10 linear monomers; all double-length molecules[r] ...
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Large T-antigen mutants define multiple steps in the initiation of simian virus 40 DNA replication.
... In the presence of RF-A, a eucaryotic single-stranded DNA-binding protein required for origin-specific unwinding and the initiation of DNA replication 23, 81, and a topoisomerase which r[r] ...
8
Widespread Phosphorylation of Histone H2AX by Species C Adenovirus Infection Requires Viral DNA Replication
... viral DNA and an abundance of single- stranded DNA (21, ...these DNA structures could stimulate a DNA damage ...Viral DNA levels were measured 24 h after infection by ...
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Process of infection with bacteriophage phi X174 XLI. Synthesis of defective phi X particles at 15 degrees C.
... Further analysis by velocity sedimentation in alkaline gradients of the single-stranded DNA after isolation by equilibrium sedimentation in neutral CsCl showed that most of the viral DNA[r] ...
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Nucleotide sequences at the phi X gene A protein cleavage site in replicative form I DNAs of bacteriophages U3, G14, and alpha 3.
... The exact position of the 4XX gene A protein nick site and the nucleotide sequence at the 3'-OH end of the nick were determined by DNA sequence analysis of the single-stranded DNA subfra[r] ...
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The Large Subunit of Replication Factor C (Rfclp/Cdc44p) Is Required for DNA Replication and DNA Repair in Saccharomyces cerevisiae
... induced single-stranded DNA breaks at the restrictive temperature is consistent with the hypothesis that Cdc44p plays a role in the DNA excision repair pathway.. DISCUSS[r] ...
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