Single stranded DNA (ssDNA)
A Phage Single-Stranded DNA (ssDNA) Binding Protein Complements ssDNA Accumulation of a Geminivirus and Interferes with Viral Movement
... circular single-stranded DNA (ssDNA) genomes encapsidated in dou- ble icosahedral ...of ssDNA in protoplasts and in plants but not for systemic infection and symptom development in ...
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Electrochemical Studies on the Recognition of a Ternary Copper Complex to Single-Stranded DNA and Double-Stranded DNA
... sperm DNA from Beijing Baitai Biochemistry Technology Company (Beijing, China) was used as ...Denatured single-stranded DNA (ssDNA) was produced by thermal denaturation [21]: ...and ...
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Displacement of the canonical single stranded DNA binding protein in the Thermoproteales
... alternative single stranded DNA binding ...for ssDNA binding proteins biochemically, we identified a single candidate, Ttx1576, which was unique to the species lacking a canonical ...an ...
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A Single-stranded DNA-binding Protein of Bacteriophage T7 Defective in DNA Annealing
... binding ssDNA and physically interacting with T7 DNA polymerase, gene ...of DNA (10, 11, 16). Homol- ogous DNA annealing is a vital activity during the process of DNA replication, ...
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Transfection of REP- mycoplasmas with viral single-stranded DNA.
... To determine if this increase in infectious centers was due to productive L51 infection of REP- cells, the experiment was repeated cells processed for transfection before addition of vir[r] ...
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In vitro synthesis of double-stranded DNA from the Kilham rat virus single-stranded DNA genome.
... SALZMAN,* PHYLLIS FABISCH, RONALD PARR, CLAUDE GARON, AND TONU WALI Laboratory of Biology of Viruses, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014 Doub[r] ...
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Robust Genome Editing with Short Single-Stranded and Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans
... basic DNA transformation protocols for ...purified DNA, too much DNA, or even specific DNA sequences, can be ...a DNA marker such as the pRF4::rol- 6(su1006) plasmid, that reports on the ...
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In Vivo Conversion of the Single-Stranded DNA of the Kilham Rat Virus to a Double-Stranded Form
... Velocity sedimentation in a 10 to 30%7O neutral sucrose gradient of Hirt supernatant fluid taken 60 min after infection from a rat nephroma cell monolayer infected with KRV containing 3H[r] ...
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An Adeno-Associated Virus (AAV) Initiator Protein, Rep78, Catalyzes the Cleavage and Ligation of Single-Stranded AAV ori DNA
... linear, single-stranded DNA (ssDNA) genome of 4,680 nucle- otides (nt) (reviewed in reference ...a single open reading frame ...cleaved DNA substrate via a phosphotyrosine ...
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Graphitic Surface Attachment by Single-Stranded DNA and Metal Nanoparticles
... 7.1 – work function and melting surface tension of selected metals 7.2 – SEM image of gold nanoparticles on 2, 3, and 13 layer graphene 7.3 – SEM imges of gold nanoparticles on 1, 2, [r] ...
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Analysis of crucial factors resulting in microarray hybridization failure
... double stranded DNA (dsDNA) were used for hybridization individually or in a ...mixture. Single stranded DNA (ssDNA) targets were isolated from selected dsDNA targets which ...
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The 2B subdomain of Rep helicase links translocation along DNA with protein displacement
... catalyse DNA and RNA strand ...from DNA ahead of advancing replication forks. How helicases catalyse DNA unwinding is increas- ingly well understood but how protein displacement is achieved is ...for ...
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Process of infection with bacteriophage phi X174 XLI. Synthesis of defective phi X particles at 15 degrees C.
... Further analysis by velocity sedimentation in alkaline gradients of the single-stranded DNA after isolation by equilibrium sedimentation in neutral CsCl showed that most of the viral DNA[r] ...
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Rapid and simple method for purification of nucleic acids
... By using size-fractionated silica particles, nucleic acids covalently closed circular, relaxed circular, and linear double-stranded DNA; single-stranded DNA; and rRNA could be purified f[r] ...
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Widespread Phosphorylation of Histone H2AX by Species C Adenovirus Infection Requires Viral DNA Replication
... viral DNA and an abundance of single- stranded DNA (21, ...these DNA structures could stimulate a DNA damage ...Viral DNA levels were measured 24 h after infection by ...
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The Large Subunit of Replication Factor C (Rfclp/Cdc44p) Is Required for DNA Replication and DNA Repair in Saccharomyces cerevisiae
... induced single-stranded DNA breaks at the restrictive temperature is consistent with the hypothesis that Cdc44p plays a role in the DNA excision repair pathway.. DISCUSS[r] ...
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Genetic Functions Promoting Homologous Recombination in Escherichia coli: A Study of Inversions in Phage Λ
... In addition to RecA protein and RecBCD enzyme, inversion by the RecBC pathway required single-stranded DNA binding protein, DNA gyrase, DNA polymerase I and DNA li[r] ...
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Single-stranded regions on unintegrated avian retrovirus DNA.
... ADDENDUM IN PROOF When the BND-cellulose chromatography procedure was applied to the unintegrated viral DNA from mouse cells infected by Moloney murine leukemia virus, 90% of the linear [r] ...
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Deoxyribonucleic acid strandedness Partial characterization of the antigenic regions binding antibodies in lupus erythematosus serum
... the DNA into a saltelutable fraction composed of primarily double-stranded molecules and an alkaline-elutable fraction containing double- stranded nucleic acid with variable length, ...
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Towards HIV Detection: Novel Poly(propylene imine) Dendrimer-Streptavidin Platform for Electrochemical DNA and gp120 Aptamer Biosensors
... The biosensor was prepared by the immobilisation of single stranded 5'- biotynilated probe DNA and B40 aptamer on the modified electrode and then used to detect complementary DNA and gp1[r] ...
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