Site-specific transgene integration
A Helper-Dependent Capsid-Modified Adenovirus Vector Expressing Adeno-Associated Virus Rep78 Mediates Site-Specific Integration of a 27-Kilobase Transgene Cassette
... for site-specific transgene ...27-kb-long transgene cassette from coinfected ...LCR-GFP transgene integration into AAVS1 was seen in 30% of analyzed integration sites, and ...
11
Efficient Site-Specific Integration of Large Transgenes by an Enhanced Herpes Simplex Virus/Adeno-Associated Virus Hybrid Amplicon Vector
... the transgene(s) are de- livered with a single HSV-1 virion, the concatemer of the packaged HSV-1 amplicon vector genome acts no differently than a single copy of the ...the transgene cassettes from each ...
8
Identification of two integration sites in favor of transgene expression in Trichoderma reesei
... on transgene expression levels have been reported to associate with chromatin structure ...fungal specific transcription factor (protein ID 68425) encoding ...high transgene expression levels in R11 ...
15
Herpes Simplex Virus Type 1/Adeno-Associated Virus rep+ Hybrid Amplicon Vector Improves the Stability of Transgene Expression in Human Cells by Site-Specific Integration
... BglII site, and the 5⬘ AAV ITR into the NotI and PacI sites of pHSVloxP ...PshAI site upstream of the 5⬘ loxP site or in the EcoRV site downstream of the 3⬘ loxP site of ...
13
Efficient Integration of Recombinant Adeno-Associated Virus DNA Vectors Requires a p5-rep Sequence in cis
... rAAV site-specific ...in integration efficiency observed when compar- ing the two partial replacement constructs of ...efficient site-specific integration of a ...the ...
11
Herpes Simplex Virus Type 1/Adeno-Associated Virus Hybrid Vectors Mediate Site-Specific Integration at the Adeno-Associated Virus Preintegration Site, AAVS1, on Human Chromosome 19
... large transgene capacity and can efficiently infect many different cell ...mediate site-specific integration of the AAV DNA into the host cell ...small transgene capacity ( ⬃ ...not. ...
11
Reduction of MBS85 gene expression after the targeted integration of a transgene into the AAVS1 site using adeno-associated virus integration machinery
... Sitespecific integration in KM102 cells: We analyzed 30 KM102 clones by Southern blotting after digestion with ...and transgene probes are indicated by ...the transgene at the AAVS1 locus and also ...
7
Targeted transgene integration overcomes variability of position effects in zebrafish
... genomic integration sites leads to variation of expression patterns of transgenes driven by engineered cis-regulatory ...targeted integration events, a simple phenotype scoring of colour change in the lens ...
10
Adeno-Associated Virus Site-Specific Integration Is Mediated by Proteins of the Nonhomologous End-Joining Pathway
... of site-specific integration in cells in which vectors had integrated and continued transgene ...between site-specific integration and random integra- tion as determined ...
10
Conditional Site-Specific Integration into Human Chromosome 19 by Using a Ligand-Dependent Chimeric Adeno-Associated Virus/Rep Protein
... Rep-mediated site-specific integration at AAVS1 takes place in transgenic rodents (mice and rats) carrying the human AAVS1 site (43), and it would be of interest to test the inte- gration ...
14
Stringent and reproducible tetracycline-regulated transgene expression by site-specific insertion at chromosomal loci with pre-characterised induction characteristics
... the transgene integration ...the transgene integration sites ...the transgene from sur- rounding sequences (and vice ...approach, site-specific recombination (SSR) is used ...
14
The human somatostatin receptor type 2 as an imaging and suicide reporter gene for pluripotent stem cell-derived therapy of myocardial infarction
... of site-specific gene-editing by ZFNs avoids these disadvantages as a single copy of the reporter gene construct is integrated in a specific target site of the DNA, in our case the safe harbor ...
15
Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes
... recognition site [60]. The exclusive presence of this site in the trans- genic construct facilitated the genetic engineering of the Y ...the transgene and insert an attP recombination signal onto the ...
8
Frequent site-specific deletion of coliphage lambda murine sarcoma virus recombinants and its use in the identification of a retrovirus integration site.
... 488-497 0022-538X/80/08-0488/10$02.00/0 Frequent Site-Specific Deletion of Coliphage X Murine Sarcoma Virus Recombinants and Its Use in the Identification of a Retrovirus Integration Sit[r] ...
10
Adeno-associated virus Rep proteins target DNA sequences to a unique locus in the human genome.
... In most of the b -galactosidase-positive clones, the entire plasmid had integrated and did so as tandem repeats. This was verified in the one clone that was analyzed in detail and sug- gested by DNA hybridization ...
9
Long-Range Targeted Manipulation of the Drosophila Genome by Site-Specific Integration and Recombinational Resolution
... an integration line from our previous study (Gao et ...an integration line from this study yielded a doublet consisting of an 600-bp product for attL and an 750-bp product for attR (lane 5), a result ...
19
The Effects of T-DNA Integration Sites on Transgene Expression in Arabidopsis
... and the distributions along the chromosomes were fairly consistent with the exception of a potential "hot spot" on chromosome VII associated with a region that may be prone to double strand breaks (DSBs) and ...
152
Transcriptional Analysis of the Adeno-Associated Virus Integration Site
... without affecting the expression level of the antisense gene. Although the mechanism of inhibition remains unclear, it is possible that the TRS-RBS region might form a stable RNA secondary structure around the ...
14
Bacteriophages
... The phage DNA is usually integrated or inserted at a specific site into the host chromosome as a prophage with the aid of integrase, and host -encoded IHF (integration host factor ) pr[r] ...
23
Ku80 Participates in the Targeting of Retroviral Transgenes to the Chromatin of CHO Cells
... of transgene ex- pression in individual transduced ...between specific enhancement of transgene expression in transduced cells or a better transduc- tion efficiency of the cell population ...
9