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Sleeping Beauty, The

The Sleeping Beauty Transposable Element:  Evolution, Regulation and Genetic Applications

The Sleeping Beauty Transposable Element: Evolution, Regulation and Genetic Applications

... Recent evidence suggests that such a scheme might be useful for generating insertional mutations in vertebrate model systems (Fischer et al., 2001). Two separate transgenic mouse lines have been established: one ...

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Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis

Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis

... Figure 4 Excision and re-integration of SB transposons in the progeny of double-transgenic hopper frogs. (a) GFP expression in sibling tadpoles derived from the outcross of an 8F hopper frog. Tadpoles 1 and 2 are ...

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Insertional Mutagenesis by a Hybrid PiggyBac and Sleeping Beauty Transposon in the Rat

Insertional Mutagenesis by a Hybrid PiggyBac and Sleeping Beauty Transposon in the Rat

... lesion. Sleeping Beauty transposase inserts Sleeping Beauty transposons into highly abundant TA sequences in the genome (Ivics et ...than Sleeping Beauty, Tol2, and Mos1 in ...

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Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli

Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli

... the sleeping beauty mutase (Sbm) operon in ...Sbm; sleeping beauty mutase), which catalyzes the isomerization of succinyl-CoA to L-methylmalonyl-CoA; (2) ygfG encoding a methylmalonyl-CoA ...

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The Sleeping Beauty Problem: What about Monday?

The Sleeping Beauty Problem: What about Monday?

... the sleeping beauty problem can give us reason to reject PP it would have to be by showing that any reasonable solution to the problem is such that conditionalizing on some admissible evidence E ...the ...

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Awakening sleeping beauty: production of propionic acid in Escherichia coli through the sbm operon requires the activity of a methylmalonyl-CoA epimerase

Awakening sleeping beauty: production of propionic acid in Escherichia coli through the sbm operon requires the activity of a methylmalonyl-CoA epimerase

... Escherichia coli is a robust microorganism. The heterolo- gous production of organic acids and alcohols by E. coli has proven to be a suitable option as evidenced by the commer- cial success of succinate production [9] ...

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A functional genomics approach to identify pathways of drug resistance in medulloblastoma

A functional genomics approach to identify pathways of drug resistance in medulloblastoma

... Historically, metastatic disease has been assumed to be highly similar to primary tumors, and therefore presum- ably equally responsive to treatments designed to target primary lesions. Using the Sleeping ...

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Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers

Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers

... Additional file 1: Supplementary Figure 1: Comparison of the IRDR- R assay with the GFP specific real-time PCR method. Selected HEK- 293 clones were examined for transposon copy numbers in parallel by the accepted green ...

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DNA transposon-based gene vehicles - scenes from an evolutionary drive

DNA transposon-based gene vehicles - scenes from an evolutionary drive

... of Sleeping Beauty-mediated gene insertion for a range of applications in various tissues, Sleeping Beauty-based vectors do indeed carry traits of their heritage and evolution that may ...

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Quantum Sleeping Beauty

Quantum Sleeping Beauty

... the Sleeping Beauty case, this means we can treat the situation as if Sleeping Beauty is told that when the coin comes up tails, she will be woken up either on Monday or on Tuesday, with equal ...

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Regulation of DNA transposition by CpG methylation and chromatin structure in human cells

Regulation of DNA transposition by CpG methylation and chromatin structure in human cells

... the Sleeping Beauty (SB) transposon and to a smaller extent the Tc3 element of Caenorhabditis elegans produced elevated transpositional activity in mouse embryonic stem (ES) cells ...

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High-performance gene expression and knockout tools using sleeping beauty transposon system

High-performance gene expression and knockout tools using sleeping beauty transposon system

... live cells. FBW7 is an F-box protein that recruits sub- strates for the SCF FBW7 E3 ubiquitin ligase. SCF FBW7 de- grades several well-known oncoproteins, including Cyclin E [18], Notch [19], c-Jun [20] and c-Myc [21]. ...

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Vectors for gene therapy: A place for DNA transposon

Vectors for gene therapy: A place for DNA transposon

... with Sleeping Beauty have been conducted in numerous cell types showing a cell-type dependent ac- tivity and the interaction with human factors such as HMGB1 and Miz-1 ...Unlike Sleeping ...

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Stable gene transfer of CCR5 and CXCR4 siRNAs by sleeping beauty transposon system to confer HIV-1 resistance

Stable gene transfer of CCR5 and CXCR4 siRNAs by sleeping beauty transposon system to confer HIV-1 resistance

... HIV/AIDS continues to be major public health threat with new infections on the rise. Current therapies do not com- pletely cure the disease and there is no effective vaccine available [1,2]. A potentially rewarding ...

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Case-oriented pathways analysis in pancreatic adenocarcinoma using data from a sleeping beauty transposon mutagenesis screen

Case-oriented pathways analysis in pancreatic adenocarcinoma using data from a sleeping beauty transposon mutagenesis screen

... the Sleeping Beauty (SB) transposon, several studies produced PDA and discovered a number of common insertion sites (CIS) and associated genes that are recurrently mutated at high ...

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The effect of music on the cardiac activity of a fetus in a cardiotocographic examination

The effect of music on the cardiac activity of a fetus in a cardiotocographic examination

... study of the relationship with the increased number of fetal movements, it should be considered whether such music could be used in a situation when it is necessary to cause the fetus to react as a result of a  ...

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Transposon Mutagenesis of the Mouse Germline

Transposon Mutagenesis of the Mouse Germline

... of these partially suppress gene expression. We can con- that gene-trap transposons are capable of mutating dis- clude from insertion 01-0032 that SB transposon gene rupted endogenous genes upon intronic insertion, re- ...

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Sleeping Beauty on Monty Hall

Sleeping Beauty on Monty Hall

... notorious Sleeping Beauty ...of Sleeping Beauty, during which she will be asked about the outcome of the coin toss that determined earlier how many times she is awakened and asked, are ...

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Aspects of gender in translations of "Sleeping Beauty"

Aspects of gender in translations of "Sleeping Beauty"

... Together with a concern to show dominant male characters in control of their emotions (the prince, for example, is not directly influenced by the princess’s beauty to kiss her as he is in some editions of the ...

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Sleeping Beauty, Mice, & Dogs: Cell Death in Narcolepsy

Sleeping Beauty, Mice, & Dogs: Cell Death in Narcolepsy

... Humans descend into REM after an hour or so of sleeping. In the drowsy period, also known as Stage I sleep, the frequency spectrum of the electroencephalogram (EEG) is shifted to lower numbers, and the amplitude ...

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