Specific PCR
Development and Validation of a HPV 32 Specific PCR Assay
... used PCR assays utilizing primers designed to detect genital HPV types (MY09/11 and PGMY primer ...type-specific PCR-based assay to detect HPV-32 for the application in a natural his- tory study of ...
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Rapid and Specific Detection of Escherichia coli Clonal Group A by Gene Specific PCR
... by PCR fingerprinting. By ERIC2 PCR, ECOR strain 47 was indistinguishable from the CGA refer- ence strains ...ERIC2 PCR) or quite closely related to CGA (by RAPD ...47 specific and were ...
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Rapid determination of clonality by detection of two closely linked X chromosome exonic polymorphisms using allele specific PCR
... sensitive, specific, reproducible, quantitative, and applicable to nonnucleated ...(13), PCR-ASOH (14), and PCR amplification of specific allele (PASA) (15) into a simpler as- say ...
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454 next generation-sequencing outperforms allele-specific PCR, Sanger sequencing, and pyrosequencing for routine <em>KRAS</em> mutation analysis of formalin-fixed, paraffin-embedded samples
... Our data show that sample enrichment is a major limiting factor for Sanger sequencing, while confirming the very high sensitivity of real-time PCR, chip array assay, pyrosequencing, and 454 next-generation ...
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The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniaeand comparison with four other species specific PCR assays
... pneumoniae PCR assays to that of a new approach, based on the 16S rRNA ...of specific primers using pub- lished Streptococcus sequences, filtered for quality and annotation-reliability by profile-based ...
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Efficiency of PCR-RFLP and Species-specific PCR for the Identification of Meat Origin in Dry Sausages
... when pork was present in meat mixtures. Moreover, Pietsch and Waiblinger (2010) reported that CYTB amplicons of the pig, turkey and chicken are ampli- fied more efficiently than those of cattle and sheep, leading to ...
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Lyme disease caused by <em>Borrelia burgdorferi</em> with two homeologous <em>16S rRNA</em> genes: a case report
... genus-specific PCR primers to perform same-nested PCR for amplification of the borrelial 16S rRNA gene, followed by direct Sanger sequencing of the PCR amplicon, can detect and validate a wide ...
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PCR Detection of Pseudoperonospora humuli and Podosphaera macularis in Humulus lupulus
... Therefore, specific PCR assays were developed to detect the pathogens Pseudoperonospora humuli and Podosphaera macularis in naturally infected hop ...The specific PCR primer combinations P1 + ...
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Evaluation of FLASH - PCR forrapid detection of Mycobacterium tuberculosis from clinical specimens.
... the PCR method for early diagnosis of ...of PCR with different DNA - extraction methods has been reported between 80-90% for MTB complex ...of PCR and primer ...as PCR single stranded ...
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Differentiation of Campylobacter jejuniSerotype O19 Strains from Non O19 Strains by PCR
... RAPD PCR (12), a PCR method using specific primers is more desirable for maximizing accuracy of ...efficient PCR amplification requires a primer whose 39-terminal nucle- otide is complementary ...
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Comparison of Different DNA Fingerprinting Techniques for Molecular Typing of Bartonella henselaeIsolates
... (REP) PCR, and arbitrarily primed (AP)-PCR, three different variants were identified among the isolates (variants I to ...type- specific amplification of 16S rDNA differentiated two types of the ...
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PCR based methods for the detection of L1014 kdr mutation in Anopheles culicifacies sensu lato
... The Leu-Phe mutation at the kdr locus in An. culicifacies s.l. was reported by Hoti et al [11] using an allele-specific PCR assay, whose external primers Agd1 and Agd2 were based on An. gambiae sequences. ...
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Measles Virus Genotyping by Nucleotide Specific Multiplex PCR
... nucleotide-specific PCR with type-specific sense primers and a common antisense primer (nt 1649 to ...(15). PCR conditions were optimized to enhance the in- hibitory effect of a mismatch at ...
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The involvement of Kras gene 3'-UTR polymorphisms in risk of cancer and influence on patient response to anti-EGFR therapy in metastatic colorectal cancer: a meta-analysis
... allele-specific PCR; PCR, polymerase chain reaction; PCR-RFLP; PCR–restriction fragment length polymorphism; BEL, Belgium Ovarian Cancer study; BWh, Brigham Women’s hospital study; DOV, ...
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Reliable allele detection using SNP-based PCR primers containing Locked Nucleic Acid: application in genetic mapping
... led to the detection of allele-specific SNPs, and AS PCR was primed at these SNPs. To maximise the selectivity of the SNP-specific PCR, 3'-LNA-modified primers proved most successful. This ...
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Use of a Genus and Species Specific Multiplex PCR for Identification of Enterococci
... genus-specific PCR primers for identification of ...multiplex PCR was determined by comparisons to standard biochemical testing, the BBL Crystal kit, VITEK, and API Rapid ID 32 ...by PCR ...
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CANINE PARVOVIRUS- AN INSIGHT INTO DIAGNOSTIC ASPECT
... real-time PCR is used for quantification of PCR product in reaction which can be used for estimation of viral load in ...time PCR (RT-PCR) has been used for the detection of CPV-2 DNA in ...
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Practical Considerations in Design of Internal Amplification Controls for Diagnostic PCR Assays
... There is a requirement to titrate the internal control DNA template prior to use in the PCR assay. For further use it is possible to prepare ready-to-go mixtures containing every re- agent, including final ...
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The role of polymorphisms of thiopurine methyltransferase in therapy with Azathioprine: preliminary study
... For the determination of the G460A genotype, a mixture of 7.0 μl of water was prepared; 2.0 μl of 3 buffers for the enzyme; 10.0 μl of PCR DNA; 1.5 μl of Mwo I New England Biolabs Enzyme. The mixture was incubated ...
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Fast Duplex One Step Reverse Transcriptase PCR for Rapid Differential Detection of West Nile and Japanese Encephalitis Viruses
... transcriptase PCR (RT-PCR) methods have been used to develop sensitive and specific assays for the identification of WNV (13, ...(TaqMan) PCR, SYBR green-based real-time PCR, and loop- ...
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