specific primers
Allele specific PCR detection of sweet cherry self incompatibility alleles S3, S4 and S9 using consensus and allele specific primers in the Czech Republic
... allele specific amplicons of alleles S 3 , S 4 and S 9 based on in- tron 1 primers in length of 303, 523 and 428 bp and based on intron 2 primers approximately 898, 1064 and 798 ...intron ...
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Development of genome specific primers for homoeologous genes in allopolyploid species: the waxy and starch synthase II genes in allohexaploid wheat (Triticum aestivum L ) as examples
... genome-specific primers for the Wx genes It has been reported that wheat varieties Sturdy and Fujimi Komugi carry the null Wx-A1 allele (Wx-A1b) [27- 29] (Table ...
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Rapid and Specific Genotyping System for Hepatitis B Virus Corresponding to Six Major Genotypes by PCR Using Type Specific Primers
... Hepatitis B virus (HBV) is a well-known agent of acute and chronic hepatitis, with an estimated 350 million chronic carri- ers around the world. HBV has a circular and partially double- stranded DNA genome of 3.2 kb ...
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Protocol: a simple gel-free method for SNP genotyping using allele-specific primers in rice and other plant species
... allele specific primer forms a perfect match at the 3' end (SNP) with Ksh DNA sequence (1) but forms a mismatch with Kal DNA ...allele specific primer similarly forms a 3' end match with Kal DNA (4) and 3' ...
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Specific Primers for Rapid Detection of Microsporum audouinii by PCR in Clinical Samples
... In conclusion, the results of our study strongly support use of the MA1 M. audouinii-specific-primers for the identification of M. audouinii in the diagnosis of tinea capitis. In the future, PCR-based ...
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Genetic identification of cryptic genospecies of Haemophilus causing urogenital and neonatal infections by PCR using specific primers targeting genes coding for 16S rRNA
... of specific primers. We selected two primers for PCR to identify the strains belonging to the cryptic genital Haemophilus ...The primers were formulated on the basis of comparison of 77 ...
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Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real time PCR with specific primers and probe
... on primers (P3 and P4) and TaqMan ™ probe (P) concentration selection criteria, which was performed according to 5 × 5 matrix of primers concen- trations ...
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Evaluation of Typing of Vibrio parahaemolyticus by Three PCR Methods Using Specific Primers
... The presence of the repeatable fingerprints in REP-PCR and ERIC-PCR suggested the presence of these repetitive consensus sequences (REP and ERIC) in V. parahaemolyticus. In another Vibrio species, V. cholerae, the ...
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Serotype Specific Identification of Polioviruses by PCR Using Primers Containing Mixed Base or Deoxyinosine Residues at Positions of Codon Degeneracy
... inosine-containing primers targeting codons of conserved amino acid sequences is a general ap- proach to the design of reagents conferring a breadth of specificity unattainable by other molecular methods (14), in- ...
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Rapid Method for Species Specific Identification ofVibrio cholerae Using Primers Targeted to the Gene of Outer Membrane Protein OmpW
... respective primers and ...toxR-specific primers, while 80 bacterial strains from other genera tested were also found to be negative by the ...
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Improved Multiplex PCR Using Conserved and Species Specific 16S rRNA Gene Primers for Simultaneous Detection ofActinobacillus actinomycetemcomitans, Bacteroides forsythus, and Porphyromonas gingivalis
... forward primers were tested with the program FastA (Genetics Computer Group) against all existing DNA sequence information stored in two databases: GenBank-EMBL and the Ribosomal Database Project ...forward ...
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Detection and Identification of Mycobacteria by Amplification of the Internal Transcribed Spacer Regions with Genus and Species Specific PCR Primers
... cies-specific primers targeting the 16S rRNA, hsp65, 32-kDa protein genes, or the internal transcribed spacer (ITS) and detect only a single or a limited number of mycobacterial species (10, ...
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Development of PCR Assays with Nested Primers Specific for Differential Detection of Three Human Anelloviruses and Early Acquisition of Dual or Triple Infection during Infancy
... species-specific primers derived from a highly conserved area located just downstream of the TATA box of the TTV, TTMDV, and TTMV genomes, a PCR method with simultaneous amplification of the genomic DNAs of ...
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PCR primers Universal primers for PCR am-
... strain-specific primers in the separate amplification of each target sequence in the ...strain-specific primers in PCR amplification of a mixed virus infection thus enabled detection of each ...
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Single-tube multiplex PCR using type-specific E6/E7 primers and capillary electrophoresis genotypes 21 human papillomaviruses in neoplasia
... general primers when product detection is limited to the most common genotypes ...capsid primers to amplify their target include relatively frequent base substitutions and deletions which may affect primer ...
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A database of PCR primers for the chloroplast genomes of higher plants
... PCR primers to new species has accelerated molecular research ...with primers is still a 'trial and error' ...tested primers for a number of species will reduce the efforts in such ...
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Detonators, Igniters, Primers, and Other Initiating Devices pdf
... The fusehead consists of a mixt of rare-earth metals & metallic peroxide, while delay compn is a mist of Sb powder & K permanganate Ref 36, p 70 An other type of British nonmilitary elec[r] ...
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Barrier and Sacrificial Protection Mechanisms of Zinc Rich Primers
... In this study, a specific type of zinc rich paint was applied on ASTM A36 steel substrate. The paint was scratched and exposed to salt fog test for up to 1,000 hours in accordance with ASTM B117. Formation of the ...
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Impact of Enzymes and Primers on the PCR Amplification of Some Goatfishes
... The polymerase chain reaction (PCR) is one of the most powerful techniques in molecular biology. In DNA isolation, substances co-extracted from biological samples, such as lipids, polysaccharides, and humic acids, ...
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Primers for Dengue Virus Strains Based on Their Sequence Variability
... selecting primers for detection, which is again critical for diagnostic ...the primers were more critical for PCR efficiency. For the forward primers, a single mismatch at position -5, -6, or - 8 of ...
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