Spindle pole
A novel small molecule inhibitor reveals a possible role of Kinesin-5 in anastral spindle pole assembly
... at spindle poles reflects spatial changes in microtubule turnover ...at spindle poles) or spatial changes in microtubule nucleation ...at spindle Fig. 4. FCPT inhibited poleward microtubule movement ...
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Sec66-Dependent Regulation of Yeast Spindle-Pole Body Duplication Through Pom152
... ABSTRACT In closed mitotic systems such as Saccharomyces cerevisiae , the nuclear envelope (NE) does not break down during mitosis, so microtubule-organizing centers such as the spindle-pole body (SPB) must ...
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The Nuclear Migration Protein NUDF/LIS1 Forms a Complex with NUDC and BNFA at Spindle Pole Bodies
... Nuclear migration depends on microtubules, the dynein motor complex, and regulatory components like LIS1 and NUDC. We sought to identify new binding partners of the fungal LIS1 homolog NUDF to clarify its function in ...
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A 2D/3D image analysis system to track fluorescently labeled structures in rod-shaped cells: application to measure spindle pole asymmetry during mitosis
... the spindle pole body (SPB), which have been labeled with a fluorescent protein tag in living cells, a number of the image analysis problems must be solved; the cell outline must be detected automatically, ...
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Stable Preanaphase Spindle Positioning Requires Bud6p and an Apparent Interaction between the Spindle Pole Bodies and the Neck
... yeast spindle pole bodies, the functional equivalent of metazoan ...given spindle pole body spends in close association with the neck region of budding ...the spindle pole body ...
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The Mitotic Exit Network Regulates Spindle Pole Body Selection During Sporulation of Saccharomyces cerevisiae
... ABSTRACT Age-based inheritance of centrosomes in eukaryotic cells is associated with faithful chromosome distribution in asymmetric cell divisions. During Saccharomyces cerevisiae ascospore formation, such an inheritance ...
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Ady3p Links Spindle Pole Body Function to Spore Wall Synthesis in Saccharomyces cerevisiae
... the spindle pole body (SPB) that is required for prospore membrane formation, and for Don1p, which forms a ring-like structure at the leading edge of the prospore membrane during meiosis ...
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PREFERENTIAL OCCURRENCE OF NONSISTER SPORES IN TWO-SPORED ASCI OF SACCHAROMYCES CEREVISIAE: EVIDENCE FOR REGULATION OF SPORE-WALL FORMATION BY THE SPINDLE POLE BODY
... Electron microscopy reveals the cytological basis for this mode of segregation: the cells undergo both meiotic divisions but only one spindle pole body (SPB) of each spindle fo[r] ...
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Mutant Membrane Protein of the Budding Yeast Spindle Pole Body Is Targeted to the Endoplasmic Reticulum Degradation Pathway
... identical spindle pole body (SPB) duplication defects: The newly formed SPB is improperly inserted into the nuclear envelope (NE), preventing the cell from forming a bipolar mitotic ...
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Phosphatidylinositol-5-phosphate 4-kinase gamma accumulates at the spindle pole and prevents microtubule depolymerization
... 3 Spindle pole‑associated MTs are less stable in PIP4KIIγ‑depleted mitotic ...100 spindle poles in three independent ...mitotic spindle after cold treatment ...the spindle ...
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Fission Yeast Mutants Affecting Telomere Clustering and Meiosis-Specific Spindle Pole Body Integrity
... In meiotic prophase of many eukaryotic organisms, telomeres attach to the nuclear envelope and form a polarized configuration called the bouquet. Bouquet formation is hypothesized to facilitate homologous chromosome ...
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subito Encodes a Kinesin-like Protein Required for Meiotic Spindle Pole Formation in Drosophila melanogaster
... the spindle poles (Matthies et ...during spindle formation of meiosis I and are dle function (Tavosanis et ...meiotic spindle (McKim and Hawley ...meiotic spindle formation begins with mosome ...
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Ady4p and Spo74p Are Components of the Meiotic Spindle Pole Body That Promote Growth of the Prospore Membrane in Saccharomyces cerevisiae
... Analysis of fluorescence intensity of GFP fusions for SPB components was performed as follows. Cells transformed with plasmids encoding GFP fusions were fixed after 6 to 8 h in 2% potassium acetate as described above, ...
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kem mutations affect nuclear fusion in Saccharomyces cerevisiae.
... Perhaps during conjugation a few of the cells in the karl-1 population have spindle pole bodies with sufficient stability to complete nuclear fusion successfully. T [r] ...
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Phosphorylation of Cdc5 regulates its accumulation
... [2], spindle-pole body separation [3], spindle position- ing [4], microtubule organization [5], recovery from hydroxyurea [6], APC/C activation [7], mitotic exit [8], cytokinesis [9,10], Cdc14 ...
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Isolation of Mutations That Bypass the Requirement of the Septation Initiation Network for Septum Formation and Conidiation in Aspergillus nidulans
... the spindle pole body (K rapp et ...stable spindle pole body components also organize regulators of SIN, such as the polo kinase Plo1p and the spindle checkpoint protein Dma1p, to the ...
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5043.pdf
... The 32 yeast kinetochores and kinetochore microtubule plus-ends, 16 on each side, are clustered into two foci each slightly larger than a diffraction-limited spot in metaphase (Haase et al., 2012). Based on the ...
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Exotic mitotic mechanisms
... Figure 3. Hypothetical overview of interactions and mechanisms that might mediate the insertion of membrane-bound kinetochores into the nuclear envelope (NE). (a) Summary of the mechanisms that lead to de novo insertion ...
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A genome-wide synthetic dosage lethality screen reveals multiple pathways that require the functioning of ubiquitin-binding proteins Rad23 and Dsk2
... the spindle pole body (SPB) duplication as a degradation target of Rad23 and Dsk2 ...in spindle regulation to affect SPB duplication, which in turn triggers growth retardation in rad23 dsk2 mutant ...
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Lesions in Many Different Spindle Components Activate the Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisiae
... the spindle the MAD- gation in cells whose spindles are defective (reviewed dependent spindle checkpoint detects, we combined in Rudner and Murray 1996; Wells ...The spindle checkpoint has been ...
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