Spindle pole body (SPB)
Ady3p Links Spindle Pole Body Function to Spore Wall Synthesis in Saccharomyces cerevisiae
... the spindle pole body (SPB) that is required for prospore membrane formation, and for Don1p, which forms a ring-like structure at the leading edge of the prospore membrane during meiosis ...
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A Novel Allele of Saccharomyces cerevisiae NDC1 Reveals a Potential Role for the Spindle Pole Body Component Ndc1p in Nuclear Pore Assembly
... the spindle pole body (SPB) and the nuclear pore complex (NPC) are essential organelles embedded in the nuclear envelope throughout the life cycle of the budding yeast Saccharomyces ...the ...
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PREFERENTIAL OCCURRENCE OF NONSISTER SPORES IN TWO-SPORED ASCI OF SACCHAROMYCES CEREVISIAE: EVIDENCE FOR REGULATION OF SPORE-WALL FORMATION BY THE SPINDLE POLE BODY
... Electron microscopy reveals the cytological basis for this mode of segregation: the cells undergo both meiotic divisions but only one spindle pole body (SPB) of each spindle fo[r] ...
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Fission Yeast Mutants Affecting Telomere Clustering and Meiosis-Specific Spindle Pole Body Integrity
... the spindle pole body (SPB), in dot mutants showed an elevated frequency of multiple Sad1-nuclei signals relative to wild ...Impaired SPB structure and function were further ...
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Mutational Analysis Reveals a Role for the C Terminus of the Proteasome Subunit Rpt4p in Spindle Pole Body Duplication in Saccharomyces cerevisiae
... The ubiquitin/proteasome pathway plays a key role in regulating cell cycle progression. Previously, we reported that a conditional mutation in the Saccharomyces cerevisiae gene RPT4/PCS1, which encodes one of six ATPases ...
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Mutant Membrane Protein of the Budding Yeast Spindle Pole Body Is Targeted to the Endoplasmic Reticulum Degradation Pathway
... identical spindle pole body (SPB) duplication defects: The newly formed SPB is improperly inserted into the nuclear envelope (NE), preventing the cell from forming a bipolar mitotic ...
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Schizosaccharomyces pombe Calmodulin, Cam1, Plays a Crucial Role in Sporulation by Recruiting and Stabilizing the Spindle Pole Body Components Responsible for Assembly of the Forespore Membrane
... Yeast is a genetically tractable model organism suitable for studying the biological function of calmodulin, using condition- al-lethal calmodulin mutants (4). In the budding yeast Saccha- romyces cerevisiae, calmodulin ...
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Sec66-Dependent Regulation of Yeast Spindle-Pole Body Duplication Through Pom152
... the spindle-pole body (SPB) must be inserted into the NE to facilitate bipolar spindle formation and chromosome ...of SPB insertion has been linked to NE insertion of nuclear ...
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Integrity and Function of the Saccharomyces cerevisiae Spindle Pole Body Depends on Connections Between the Membrane Proteins Ndc1, Rtn1, and Yop1
... the SPB with the shmoo extension and astral microtubules that extend into the ...minor spindle positioning defect (Table ...bar-like spindle posi- tioned at the bud neck, revealed additional defects ...
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The Mitotic Exit Network Regulates Spindle Pole Body Selection During Sporulation of Saccharomyces cerevisiae
... new SPB have been observed in mitosis and impact on mitotic spindle polarity (Juanes et ...age-based SPB selection and localization of Spc72 to SPBs during meiosis I has been reported before (Gordon ...
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Fine Structure Analysis of the Yeast Centrin, Cdc31p, Identifies Residues Specific for Cell Morphology and Spindle Pole Body Duplication
... AAA SPB- nor Kic1p-related defects, providing candidates for TTA TTA CGA TAA ATC AAT ATC CGT TTT AAG AGC TTG GTG-3⬘)/PR303 (5⬘-CAT GGT GGT CAG CTG GAA TTC GAT future exploration of novel ...
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Ady4p and Spo74p Are Components of the Meiotic Spindle Pole Body That Promote Growth of the Prospore Membrane in Saccharomyces cerevisiae
... Another mutant in which prospore membranes fail to cap- ture nuclei that may shed more light on the function of Ady4p is the spo20⌬ mutant. As in ady4⌬/ady4⌬ cells, prospore mem- branes in spo20⌬/spo20⌬ cells are ...
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A genome-wide synthetic dosage lethality screen reveals multiple pathways that require the functioning of ubiquitin-binding proteins Rad23 and Dsk2
... the spindle pole body (SPB) duplication as a degradation target of Rad23 and Dsk2 ...in spindle regulation to affect SPB duplication, which in turn triggers growth retardation in ...
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A 2D/3D image analysis system to track fluorescently labeled structures in rod-shaped cells: application to measure spindle pole asymmetry during mitosis
... the spindle pole body (SPB), which have been labeled with a fluorescent protein tag in living cells, a number of the image analysis problems must be solved; the cell outline must be detected ...
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Phosphorylation of Cdc5 regulates its accumulation
... [2], spindle-pole body separation [3], spindle position- ing [4], microtubule organization [5], recovery from hydroxyurea [6], APC/C activation [7], mitotic exit [8], cytokinesis [9,10], Cdc14 ...
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Isolation of Mutations That Bypass the Requirement of the Septation Initiation Network for Septum Formation and Conidiation in Aspergillus nidulans
... putative spindle pole bodies at the ends of the spindle (Figure ...two spindle pole bodies (Figure ...the spindle pole body (Figure ...the spindle ...
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Requirement for the Budding Yeast Polo Kinase Cdc5 in Proper Microtubule Growth and Dynamics
... abnormal spindle morphology, shorter spindle length, and delayed microtubule regrowth at the nonpermissive ...the spindle pole body components Nud1, Slk19, and Stu2 in ...
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Lesions in Many Different Spindle Components Activate the Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisiae
... functional spindle Tubulin and spindle pole body mutants activate the (Figure ...the spindle checkpoint: The mad mutants were originally isolated as mutants that had increased ...
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Mitosis, Not Just Open or Closed
... when spindle microtubules have attached to the appro- priate kinetochores ...metaphase spindle has formed or maintain their nuclear envelope throughout ...the spindle apparatus and chromosomes within ...
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A RAD9-dependent checkpoint blocks meiosis of cdc13 yeast cells.
... cdc39) also display meiotic arrest at a point following spindle pole body duplication, but these latter mutants differ in that they proceed farther, undergoing chro- moso[r] ...
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