Spliced leader
Trypanosome Spliced-Leader-Associated RNA (SLA1) Localization and Implications for Spliced-Leader RNA Biogenesis
... H/ACA RNA is a group of small nucleolar RNAs that direct pseudouridylation on rRNA and snRNAs. In almost all eu- karyotes, these RNAs consist of two stem-loop structures con- nected by a single-stranded hinge and a tail ...
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A Divergent Transcription Factor TFIIB in Trypanosomes Is Required for RNA Polymerase II-Dependent Spliced Leader RNA Transcription and Cell Viability
... Trypanosomes have unusual ways of expressing genes: polycis- tronic pre-mRNAs become stable, translatable mRNAs only after the addition of a 5 ⬘ capped spliced leader (SL) sequence and a 3 ⬘ polyadenylated ...
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Actively Transcribing RNA Polymerase II Concentrates on Spliced Leader Genes in the Nucleus of Trypanosoma cruzi
... the spliced leader RNA, which are capped, exported to the cytoplasm, processed, and reimported into the nucleus before they are used as splicing donors to form mRNAs from pre-mRNA polycistronic ...and ...
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Spliced Leader RNA Gene Transcription in Trypanosoma brucei Requires Transcription Factor TFIIH
... by spliced leader (SL) trans splicing and ...trans spliced, the parasites depend on strong and continuous SL RNA synthesis mediated by RNA polymerase ...
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Spliced leader RNA silencing (SLS) a programmed cell death pathway in Trypanosoma brucei that is induced upon ER stress
... Trypanosoma brucei is the causative agent of African sleeping sickness. The parasite cycles between its insect (procyclic form) and mammalian hosts (bloodstream form). Trypanosomes lack conventional transcription ...
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Heterodera glycines utilizes promiscuous spliced leaders and demonstrates a unique preference for a species specifc spliced leader over C elegans SL1
... spliced leader trans-splicing (sLts) plays a part in the maturation of pre-mRNAs in select species across multiple phyla but is particularly prevalent in ...of spliced leaders (sL) within the cell is ...
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Exportin 1 Mediates Nuclear Export of the Kinetoplastid Spliced Leader RNA
... brucei cells, we performed primer extensions on SL RNA with oligo- nucleotides specific for stem-loops I, II, or III in the nascent- enriched nuclear fraction, the cytoplasmic fraction, [r] ...
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Operons Are a Conserved Feature of Nematode Genomes
... to spliced leader RNAs, and we are able to detect polycistronic RNAs derived from these ...“spliced leader” (SL) trans-splicing predate the radiation of the nematode phylum, an inference which ...
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Evaluation of Four Single Locus Markers for Leishmania Species Discrimination by Sequencing
... Several genetic markers have been described for discriminating Leishmania species. In most reported cases, one or a few poly- morphisms are the basis of species identification, and the methods were validated on a limited ...
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Comparison of PCR methods for detection of Leishmania siamensis infection
... siamensis in this study, which amplified different target genes, i.e., the cpb, the cyt b, the ITS1 region of the SSUrRNA, the hsp70, the spliced leader mini-exon, the SSUrRNA and the ti[r] ...
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The leader sequence of the subgenomic mRNA's of Rous sarcoma virus is approximately 390 nucleotides.
... We found that the subgenomic mRNA's that direct the synthesis of the env and src gene products have uninterrupted spliced leader sequences of approximately 390 nucleotides, whereas virio[r] ...
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cis Elements Required for High-Level Expression of Unspliced gag-Containing Message in Moloney Murine Leukemia Virus
... from CAG GCT (GlnAla) to CAA CTT (GlnLeu) at the sec- ond SA site (wtAg01) resulted in splicing by using the two further downstream AG’s (Fig. 4, lane 6; the two SA sites which were determined by RT-PCR amplification of ...
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Kirk_unc_0153D_18462.pdf
... the spliced isoforms of each ...canonical spliced annotations, if it exists, for each ...the spliced trancript by dividing the FeatureCounts value by the length of the ...this spliced RPBP ...
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Control of mRNA Stability in Fungi by NMD, EJC and CBC Factors Through 3′UTR Introns
... Our data also demonstrate a second branch of NMD in which an intron downstream of a termination codon elicits NMD and requires EJC and CBC factors as well as at least UPF1 and UPF2. This second branch would have a role ...
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Spliced transcripts of human cytomegalovirus.
... A subset of the large number of possible splices predicted was then studied by RT-PCR of splice sites with first-strand cDNA from IE, early, and late times as template.. This identified [r] ...
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IRE1α XBP1 pathway promotes melanoma progression by regulating IL 6/STAT3 signaling
... The UPR of mammalian cells is initiated by three ER transmembrane proteins: activating transcription factor 6 (ATF6), inositol-requiring enzyme 1 (IRE1), and double- stranded RNA-activated protein kinase-like ER kinase ...
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Mutations in the regions of the Rous sarcoma virus 3' splice sites: implications for regulation of alternative splicing.
... The relative amount of spliced src mRNA in pMPM7-transfected cells determined from the ratio of unspliced src to spliced src was increased from 12 to 28%, but the overall level of splice[r] ...
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trans-Acting Inhibition of Genomic RNA Dimerization by Rous Sarcoma Virus Matrix Mutants
... When we performed RPA using a riboprobe designed to differentiate between spliced and unspliced viral RNAs, we found that the intracellular ratio of unspliced to spliced RNA was about th[r] ...
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The herpes simplex virus regulatory protein ICP27 contributes to the decrease in cellular mRNA levels during infection.
... Because the majority of herpes simplex virus type 1 HSV-1 transcripts are not spliced 60, whereas the majority of cellular transcripts are spliced, we have considered the possibility tha[r] ...
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Identification of alternatively spliced mRNAs encoding potential new regulatory proteins in cattle infected with bovine leukemia virus.
... 67, 1993 ALTERNATIVELY SPLICED mRNAs OF BOVINE LEUKEMIA VIRUS In summary, the in vitro data indicated that the alternative spliced mRNAs, as described for the Tax/Rex mRNA 42, 76, 77, ca[r] ...
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