SRB, sulforhodamine B
Cytotoxicity evaluation of a Thai herb using tetrazolium (MTT) and sulforhodamine B (SRB) assays
... The sulforhodamine B (SRB) protein stain is used for in vitro chemosensitivity test- ...The SRB assay appears to be more sensitive than the MTT assay, with a better linearity with cell number ...
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Detection on Antioxidant and Cytotoxicity Activities of Exopolysaccharides Isolated in Plant-Originated Lactococcus lactis
... The sulforhodamine B (SRB) assay was used in the study according to the method of Longo-Sorbello with a slight modification (Longo-Sorbello et ...
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Cytotoxicity Study of Gold Nanoparticles on the Basal Like Triple Negative HCC 1937 Breast Cancer Cell Line
... Sulforhodamine B (SRB) is an anionic dye that binds electrostatically to cellular proteins, reflecting indirectly the number of living cells in the ...of SRB in 1% (v/v) acetic acid was added ...
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In vitro cytotoxicity of Calotropis procera and Trigonella foenum graecum against human cancer cell lines
... The anticancer activity was determined by the cytotoxic potential of the test material using human cancer cell lines which were allowed to grow on tissue culture plates in the presence of test material. The cell growth ...
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Design, synthesis and biological evaluation of some novel Schiff base derivatives as potential anticancer agents
... Novel series of pyrrolizine Schiff bases has been synthesized then biologically evaluated as potential anticancer agents. The starting compounds,7-cyano-6-amino-N-(4-(un)substituted-phenyl)-2,3-dihydro-1H-pyrrolizine-5- ...
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IN VITRO SULFORHODAMINE B ASSAY EVALUATION OF NOVEL 2-PHENYL BENZOFURANONE DERIVATIVES ON HUMAN SKIN CANCER CELL LINE G361.
... 0.04% SRB solution was added to each well and incubated and kept at room temperature for 1 hour and quickly rinse the plates washed four times with 1%(v/v) acetic acid (200microliter) to remove unbound ...
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IN VITRO ANTICANCER ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF BACOPA MONNIERI (L ) WETTST
... Sulforhodamine B (SRB) Assay: In-vitro SRB Assay of the prepared extracts was performed on the various Human Cancer Cell Lines at Tata Memorial Centre – Advanced Centre for Treatment, Research ...
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Redox effects and cytotoxic profiles of MJ25 and auranofin towards malignant melanoma cells
... Figure 1: MJ25 activates p53 and eradicates melanoma cells in culture. a. Chemical structure of MJ25. b. ARN8 cells, which express wt p53 and have been stably transfected with a p53-dependent β-galactosidase ...
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“Synthesis and Antitumor Activity of Novel Pyrazolo[3,4-D]Pyrimidine and their Tricyclic Derivatives” by Phoebe, F. Lamie, Egypt.
... The cytotoxic activity of the newly synthesized compounds was examined in vitro on human breast adenocarcinoma cell line (MCF7) using Sulforhodamine-B stain (SRB) assay a[r] ...
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“Synthesis of New Poly Amide linked Heterocyclesas G-quadruplex Stabilizing Agents” by Kawkab Saour, Dunya Lafta, Iraq.
... The short-term cell growth inhibitory activity of the biaryl ligands was evaluated using the sulforhodamine B (SRB) assay for three different cancer cell lines and a normal human lung fibroblast cell ...
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Effects of Sulfate-Reducing Bacterial on Corrosion of 403 Stainless Steel in Soils Containing Chloride Ions
... a result of the actions of chloride and metabolic sulfide ions. According to the electrochemical mechanism of the pitting formation, two different reaction regions exist on the surface of the electrodes: one, anode, is ...
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The Effect of Mn2+ Concentration on Mn Removal by a Sulfate Reducing Bacteria Bioreactor
... in SRB bioreactors, 6,22,23) we have investigated the conditions for Mn removal by SRB, and it was found that co-existence of other heavy metal ions and citrate (a component of the SRB medium) ...
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Antimicrobial action and anti-corrosion effect against sulfate reducing bacteria by lemongrass (Cymbopogon citratus) essential oil and its major component, the citral
... was established at 0.17 mg ml -1 according to the spectrophotometric assay result (Figure 1A), while the real time PCR of dsrA gene indicated a MIC of 0.085 mg ml -1 (Figure 1B). Thus, citral was evinced to be respon- ...
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Strong coupling between surface plasmon polaritons and Sulforhodamine 101 dye
... In this article, we report on experiments showing the strong coupling between SPPs and Sulforhodamine 101 (SR101), which is an organic semiconductor that has two absorption maxima at around 550 and 600 nm a and ...
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Diversity and community pattern of sulfate-reducing bacteria in piglet gut
... of SRB in metabolism, SRB is also implicated in IBD ...an SRB mix- ture obtained from colitis patients to germ-free mice led to the activation of T cells and upregulated IL-6, IL-17, IFN- γ , and ...
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Failure Analysis: Case Study Challenger SRB Field Joint
... III. F AULT T REE A NALYSIS OF THE C HALLENGER F UILURE The FTA serves as a detailed diagram linking specified events to an overall fault based on system analysis through cause and effect. It covers both technical ...
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PHYTOCHEMICAL AND BIOLOGICAL INVESTIGATION OF URTICA URENS L. GROWING IN EGYPT
... using SRB assay method was carried out on the total methanol extract in addition to four different fractions (petroleum ether, methylene chloride, ethyl acetate and n-butanol) against four different cell ...
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Evaluating the Microbial Community and Gene Regulation Involved in Crystallization Kinetics of ZnS Formation in Reduced Environments
... in SRB abundance, as acetate would be an easily-utilized carbon substrate for these ...final SRB activity did not seem to be affected based on sulfate-removal rates that were consistent across metal and ...
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THERAPEUTIC APPLICATIONS OF BIOACTIVE COMPOUNDS OBTAINED BY ENDOPHYTIC Thielaviopsis basicola ISOLATED FROM Ximenia americana, WESTERN GHATS OF INDIA
... All chemicals used in the present study were purchased from Sigma- Aldrich, USA and Hi-media, India. The chemicals like 2, 2-diphenyl-1-picrylhydrazyl The objective of the present study was the bioprospection of ...
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Economic stress or random variation? Revisiting German reunification as a natural experiment to investigate the effect of economic contraction on sex ratios at birth
... the SRB over time, our re-analysis of the German re- unification case to look at the effects of economic con- traction on SRB provides little support for the economic stress hypothesis ...low SRB in ...
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